Arctic LTER Database

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The re-use of scientific data has the potential to greatly increase communication, collaboration and synthesis within and among disciplines, and thus is fostered, supported and encouraged. Permission to use this dataset is granted to the Data User free of charge subject to the following terms:

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Shaver, G. 1989. Above ground biomass in acidic tussock tundra experimental site, 1989, Arctic LTER, Toolik, Alaska. Arctic LTER, Marine Biological Lab, Woods Hole, Ma 02543. 1989gsttbm http://ecosystems.mbl.edu/arc/terrest/biomass/index.shtml 

4 ) Acknowledgement. The Data User should acknowledge any institutional support or specific funding awards referenced in the metadata accompanying this dataset in any publications where the Data Set contributed significantly to its content. Acknowledgements should identify the supporting party, the party that received the support, and any identifying information such as grant numbers. For example:

Data sets were provided by the Arctic LTER. This material is based upon work supported by the National Science Foundation under Grants #DEB-981022, 9211775, 8702328; #OPP-9911278, 9911681, 9732281, 9615411, 9615563, 9615942, 9615949, 9400722, 9415411, 9318529; #BSR 9019055, 8806635, 8507493.

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Dataset URLs:METADATA: HTML, Rich Text, XML(EML compliant)
DATA: Comma Delimited, Excel file with Metadata and data, Dataset via LTER Data Poral
Dataset ID:2010_EVRT1980_STOMATAL_DENSITY.02
Dataset Title:Somatal length and density in 2010 for the 1980-82 Eriophorum vaginatum reciprocal transplant experiment
Investigator 1: 
First Name:Ned
Last Name:Fetcher
Organization:Wilkes University
Address line 2:Institute for Environmental Science and Sustainabilty
Address line 3:84. W. South St.
City:Wlkes-Barre
State:PA
Zip Code:18766
Country:USA
Investigator 2: 
First Name:James
Last Name:McGraw
Organization:West Virginia University
Address line 2:Department of Biology
Address line 3:53 Campus Drive
City:Morgantown
State:WV
Zip Code:26505
Country:USA
Investigator 3: 
First Name:Gaius
Last Name:Shaver
Organization:Marine Biological Laboratory
Address line 2:The Ecosystem Center
Address line 3:
City:Woods Hole
State:MA
Zip Code:02543
Country:USA
Investigator 4: 
First Name:Cynthia
Last Name:Bennington
Organization:Stetson University
Address line 2:Department of Biology
City:DeLand
State:FL
Zip Code:32723
Country:USA
Investigator 5: 
First Name:Caitlin
Last Name:Peterson
Organization:Stetson University
Address line 2:Department of Biology
Address line 3:421 N Woodland Blvd Unit 8264
City:DeLand
State:FL
Zip Code:32723
Country:USA
Investigator 6: 
First Name:Hilary
Last Name:Harold
Organization:Glenville State University
Address line 2:Department of Land Resources
Address line 3:200 High Street
City:Glenville
State:WV
Zip Code:26351
Country:USA
Associate Investigators:
Keywords:stomatal conductance, cline, stomatal density, stomatal length, Eriophorum vaginatum, 1980 Eriophorum vaginatum transplant experiment, reciprocal transplant, tussock temperature, populations
Abstract: These data were collected in July 2010 for tussocks transplanted in 1980-82 in a reciprocal transplant experiment and harvested in 2011. Important variables are garden name, source population, length and density of stomata, and the temperature of tussocks.
For questions about the Metadata and data contact the Investigators.
For information about this web site contact:
Arctic LTER Information Manager
The Ecosystems Center
Marine Biological Lab
7 MBL St
Woods Hole, MA 02543
Phone (508) 289 7496
Email: arc_im@mbl.edu
Online URL: http://ecosystems.mbl.edu/ARC/
DATA FILE INFORMATION:
Data File URL http://metacat.lternet.edu/das/dataAccessServlet?docid=knb-lter-arc.10526&urlTail=ecotypes/transplant_garden/data/2010_EVRT1980_STOMATAL_DENSITY.csv
Data File Name 2010_EVRT1980_STOMATAL_DENSITY
Beginning Date 7/12/2010
End Date 7/22/2010
Number of Data Records 224
Other Files to Reference
Availability Status 1
Version 2: Checked keywords against the LTER network preferred list and replaced non-preferred terms. Jim L 27Jan14
Quality Control Information
Maintenance Description
Log of Changes:
 
RESEARCH LOCATION:                  
Location Name Eagle Creek Transplant Garden No Name Creek Transplant Garden Coldfoot Transplant Garden Toolik Lake Transplant Garden Sagwon Transplant Garden Prudhoe Bay Transplant Garden Select Site or enter New One Select Site or enter New One  
Geographic Description Eagle Creek Tussock Tundra Site on hillside above Eagle Creek at Mile 101 of the Steece Highway; garden is about 500 m upslope from an old berm created by bulldozer. Coordinates are approximate. Coordinates and elevation for all sites are from Google Earth. The garden is in the middle of giant tussocks 100 m north and 165 m west of the bridge that crosses No Name Creek on the Dalton Highway. Access is easier by going along No Creek for 165 m and then heading north. The garden is 100 m north of the edge of the forest and 190 m east of the Dalton Highway. The garden is in the complex of "historic sites" at Toolik Lake. The garden is 50 m north of the upper parking area at the Sagwon Overlook pulloff. Site fomerly known as MS 127. The garden is 1.5 km west of the Kuparik Bridge and 420 m north of the road on the east bank of a stream. Note: Permission from BP and oil field safety training required to access this site. Enter Description Enter Description  
Location Bounding Box                  
West Bounding Coordinate                  
East Bounding Coordinate                  
North Bounding Coordinate                  
South Bounding Coordinate                  
OR if single point location                  
Latitude 65.433665 66.116744 67.258973 68.629538 69.424431 70.328353 In Decimal Degrees In Decimal Degrees  
Longitude -145.514261 -150.169792 -150.169153 -149.579858 -148.709647 -149.062722 In Decimal Degrees In Decimal Degrees  
Elevation 762 167 319 759 269 8 In Meters In Meters  
Link to Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map      
                   
 
TAXONOMIC COVERAGE:
Organisms studied Eriophorum vaginatum
 
Methods:In 1980-1982, six transplant gardens were established along a latitudinal gradient in interior Alaska from Eagle Creek, AK, in the south to Prudhoe Bay, AK, in the north (Shaver et al. 1986, Bennington et al. 2012) .Three sites, Toolik Lake (TL), Sagwon (SAG), and Prudhoe Bay (PB) are north of the continental divide and the remaining three, Eagle Creek (EC), No Name Creek (NN), and Coldfoot (CF), are south of the continental divide. Each garden consisted of 10 individual tussocks transplanted back to their home-site, as well as 10 individuals from each of the other transplant sites (n = 10; 6 populations x 6 sites x 10 replicates = 360 total individuals).

For each E. vaginatum tussock sampled, transparent impressions were created of the widest abaxial surface of three haphazardly chosen leaves using clear nail polish. The nail polish peels were mounted on glass slides under clear plastic tape for viewing with a light microscope. Stomata were viewed at 50 magnification and photographed after which AxioVision software (ver. 3.1, Carl Zeiss Vision Imaging Systems, Germany) was used to digitally manipulate the photos. Because stomata in monocots are arranged in linear rows or files, one file was chosen from each leaf impression and an area (minimum= 0.05 mm 2 , maximum = 0.25 mm 2 ), determined by the quality of the peel and/or the availability of undamaged portions, was delineated around it, oriented in the same direction as the file and including the adjacent, stomata-freearea. All the stomata within this area were counted as an estimate of stomatal density (SD) for the leaf. Only stomata in one location per leaf were counted as within-leaf variation in density tended to be very low compared to between-leaf variation. Stomatal length (SL) was expressed as the length in micrometers of the guard cells on a closed stomate at the same magnification. Stomatal density and length are important determinants of stomatal conductance. According to theory (Brown and Escombe 1900, Franks and Beerling, 2009 ): Conductance (C) =(SD * Pore Area)/Effective Pore Depth. Effective pore depth is assumed to be relatively constant across stomata within E. vaginatum and pore area is assumed to be proportional to SL 2 . Thus, an index of conductance is calculated as C = SD*SL2 .

In 14-19 July 2011, the temperature of each tussock was taken by inserting a digital thermometer 10 cm into the top of each tussock.

Bennington CC, Fetcher N, Vavrek MC, Shaver GR, Cummings KJ, McGraw JB. 2012. Home site advantage in two long-lived arctic plant species: results from two 30-year reciprocal transplant studies. Journal of Ecology 100:841-851

Brown , H. T., F. Escombe. 1900. Static diffusion of gases and liquids in relation to the assimilation of carbon and translocation in plants. Philosophical Transactions of the Royal Society of London, B, Biological Sciences 193: 223 291.

Fetcher, N., and G. R. Shaver. 1990. Environmental sensitivity of ecotypes as a potential influence on primary productivity. American Naturalist 136:126-131.

Franks , P. J., D. J. Beerling. 2009. Maximum leaf conductance driven by CO2 effects on stomatal size and density over geologic time. Proceedings of the National Academy of Sciences, USA 106 : 10343 10347 .

Peterson, C. A. , N. Fetcher, J. B. Mcgraw , and C. C. Bennington. 2012. Clinal variation in stomatal characteristics of an arctic sedge, Eriophorum vaginatum (Cyperaceae). American Journal of Botany 99: 110.

Shaver GR, Fetcher N, Chapin FS 1986. Growth and flowering in Eriophorum vaginatum - Annual and latitudinal variation. Ecology 67:1524-1535

Notes:
Funding for this research was provided by National Science Foundation grant ARC-0908936.
Stomatal data are incorporated in Peterson et al. 2012.

Data Table

Variable Name Variable Description Data Type Units DateTime Format Code Information Missing Value Code
Garden Name of the transplant garden text     EC= Eagle Creek| NN= No Name Creek| CF= Coldfoot| TL= Toolik Lake| SAG= Sagwon| PB= Prudhoe Bay  
Source Name of the source population. text     EC= Eagle Creek| NN= No Name Creek| CF= Coldfoot| TL= Toolik Lake| SAG= Sagwon| PB= Prudhoe Bay  
Tussock Number of tussock number dimensionless     .=missing or not measured
Home/Away Whether tussock was transplanted back into the source garden (Home) or an alien garden (Away) text     H= Home| A= Away  
SourceDegreeDays No. of thawing degree days at the garden (from Fetcher and Shaver 1990) number celsius   N= North| S= South .=missing or not measured
N Number of observations per tussock number dimensionless   N= North| S= South .=missing or not measured
GardenDegreeDays No. of thawing degree daysfor the source population (from Fetcher and Shaver 1990) number celsius   1= Alive| 0= Dead or Missing .=missing or not measured
Stomatal Density Mean number of stomata per mm2 for the tussock number numberPerMillimeterSquared   1= Alive| 0= Dead or Missing .=missing or not measured
Stomatal Length Mean length of stomata for the tussock number micrometer   1= Found| 0= Missing .=missing or not measured
LxD Length x Density number dimensionless   1= Alive| 0= Dead or Missing .=missing or not measured
Cond_Transform Density x Length2 number dimensionless   1= Alive| 0= Dead or Missing .=missing or not measured
TussockTemp2011 Temperature measured in 2011 at 10 cm depth in the tussock number celsius     .=missing or not measured