98hobact
Number of bacteria in Toolik Lake water column during May, June, July and August 1998 , Arctic LTER 1998.
John
Hobbie
Michele
Bahr
additional investigator
Number of bacteria in Toolik Lake water column during May, June, July and August 1998
bacteria
picoplankton
Acceptance and utilization of LTER data requires that:
The Principal Investigator be sent a notice stating
reasons for acquiring any data and a description of the
publication intentions.
The Principal Investigator of the data set be sent a
copy of the report or manuscript prior to submission and be
adequately cited in any resultant publications
A copy of any resultant publications should be sent to:
Principal Investigator
Ecosystems Center
Marine Biological Laboratory
Woods Hole, MA 02543
http://ecosystems.mbl.edu/ARC/data/98hobact.dat
Toolik Lake, Camp Pond
-149.75
-149.0433
68.8
68.5
610
1360
meter
1998
Log of changes:
For Archival Use:
DATE RECEIVED: Feb 22, 2001
DATA FILE ENTERED BY:
DATA FILE VALIDATION:
NAME: Neil Bettez
DATE:
Data Manager
The Ecosystems Center
Marine Biological Lab
7 MBL St
Woods Hole
MA
02543
USA
(508) 289 7496
arc_im@mbl.edu
http://ecosystems.mbl.edu/ARC/
ARC LTER
The Ecosystems Center
Marine Biological Lab
7 MBL St
Woods Hole
MA
02543
USA
(508) 289 7496
arc_im@mbl.edu
http://ecosystems.mbl.edu/ARC/
After transport to the laboratory at Woods Hole, MA, 2 ml of glutaraldehyde-preserved lake water from each depth was mixed with 2 ml 0.2 um filtered phosphate buffered saline to reduce humic interference and 0.1% Triton X-100 or Nonidet P-40 to improve cell permeability and stored overnight at 4*C. The sample was stained with 200 ul 100ug/ml 4'6 diamidino-2-phenylindole (DAPI), final concentration 10 ug/ml. After 5 minutes, the sample was vacuum filtered through a 0.22 um pore size black Nuclepore 25mm filter in a 15 ml Millipore filtration apparatus. The filter was mounted on a microscope slide using Cargille A immersion oil. Ten fields were counted using a Zeiss epifluoresencce microscope.
CALCULATIONS
Variable Formula
cell count (x/field)(2.04 x 10^4 field/ml) = #/ml
Reference Citations: Hobbie, J.E., T.L. Corliss and B.J. Peterson, Seasonal patterns of bacterial abundance in an
Arctic lake. 1983. Arctic and Alpine Research 15:252-259.
Porter, K.G. and Y.S.Feig The use of DAPI for identifying and counting aquatic microflora.
1980. Limnology and Oceanography 25:943-948.
GEO-1
uid=ARC,o=lter,dc=ecoinformatics,dc=org
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public
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Variable Variable descrip. Precis./Units Coded Missing
SITE site of measurement y
DATE Date DD-MMM-YY n
STATION location at lake
DEPTH meters
COUNT # cells x 10^6/ml 0.00
Aquatic-Lakes