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Arctic LTER Database
Arctic LTER Database
Acceptance and utilization of LTER data requires that:
(1) The Principal Investigator be sent a notice stating reasons for acquiring any data and a description of the publication intentions.
(2) The Principal Investigator of the data set be sent a copy of the report or manuscript prior to submission and be adequately cited in any resultant publications.
(3) A copy of any resultant publications should be sent to:
Principal Investigator
Ecosystems Center
Marine Biological Laboratory
7 MBL St.
Woods Hole, MA 02543
Older Metadata: Attribute table not in XML (EML) file
| Dataset URLs: | METADATA: HTML, Rich Text, XML(EML compliant)
DATA: Comma Delimited, Excel file with Metadata and data |
| Dataset ID: | 96hocyan |
| Dataset Title: | Number of cyanobacteria in Toolik Lake at 1 meter depth during June, July and August 1996 , Arctic LTER 1996. |
| Investigator 1: | |
| First Name: | John | | Last Name: | Hobbie | | Address line 1: | | | Address line 2: | | | Address line 3: | | | City: | | | State: | | | Zip Code: | | | Country: | | | Associate Investigators: | Michele Bahr |
| Keywords: | bacteria, picoplankton |
| Abstract: |
Number of cyanobacteria in Toolik Lake at 1 meter depth
during June, July and August 1996 |
| Contact: |
Arctic LTER Information Manager
The Ecosystems Center
Marine Biological Lab
7 MBL St
Woods Hole, MA 02543
Phone (508) 289 7496
Email: arc_im@mbl.edu
Online URL: http://ecosystems.mbl.edu/ARC/ |
| DATA FILE INFORMATION: |
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| Data File Name |
96hocyan |
| Beginning Date |
5/1/1996 |
| End Date |
9/1/1996 |
| Number of Data Records |
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| Other Files to Reference |
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| Availability Status |
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| Quality Control Information |
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| Maintenance Description |
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| Log of Changes: |
Log of changes:
For Archival Use:
DATE RECEIVED:
DATA FILE ENTERED BY:
DATA FILE VALIDATION:
NAME:
DATE: |
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| RESEARCH LOCATION: |
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| Geographic Description |
Toolik Lake |
| Location Bounding Box |
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| West Bounding Coordinate |
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| East Bounding Coordinate |
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| North Bounding Coordinate |
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| South Bounding Coordinate |
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| OR if single point location |
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| Latitude |
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| Longitude |
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| TAXONOMIC COVERAGE: |
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| Organisms studied |
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| Methods: |
Lake water was collected from the Toolik Lake main station at 1 m depth and preserved with 2%
glutaraldehyde. Samples were transported to the Dept. of Fisheries and Oceans in West Vancouver, British
Columbia, Canada for analysis. Twenty ml aliquots were filtered onto 0.2 black polycarbonate membrane
using a 25 mm filterholder, with vacuum pressure kept to less than 5 kPa. Filters were transferred to a
microscope slide and mounted with nonfluorescent immersion oil. Cells were counted at 1000x
magnification using a green-yellow (520-560 nm) excitation filter with an epifluorescence microscope.
Cell volumes were estimated by measuring the dimensions of subsets of cells with an eyepiece micrometer.
Calculations for cell volumes were done using formulae for geometric shapes approximating the shapes of
the cells. Synechococcus and Microcolonial Synechococcus assumed spheres with average cell
diamter 0.9 mm; Oscillatoria assumed cylinder with 0.8 mm width x average length.
Reference Citations: MacIsaac, E.A. and J.G. Stockner. 1993. Enumeration of phototrophic picoplankton by autofluorescence
microscopy. In Kemp, P., B.F. Sherr, E.B. Sherr and J.J. Cole (eds.) Handbook of Methods in Aquatic
Microbial Ecology, lewis Publishers, Boca Raton, pp. 187-197.
Sampling Description.
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Data Table
Variable Variable descrip. Precis./Units Coded Missing
SITE site of measurement
DATE Date MM/DD/Y
DEPTH meters
CELL TYPE Genus
BIOVOLUME average cell biovolume in mm3
COUNT number of cells per ml numberpermilliliter
BIOMASS total cell biovolume (mm3) per ml
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