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Arctic LTER Database

Arctic LTER Database

Acceptance and utilization of LTER data requires that:

(1) The Principal Investigator be sent a notice stating reasons for acquiring any data and a description of the publication intentions.
(2) The Principal Investigator of the data set be sent a copy of the report or manuscript prior to submission and be adequately cited in any resultant publications.
(3) A copy of any resultant publications should be sent to:

Principal Investigator
Ecosystems Center
Marine Biological Laboratory
7 MBL St.
Woods Hole, MA 02543

Dataset URLs:METADATA: HTML, Rich Text, XML(EML compliant)
DATA: Comma Delimited, Excel file with Metadata and data
Dataset ID:arctic_isotopes.01
Dataset Title:Concentration of dissolved inorganic carbon (DIC), carbon and nitrogen concentrations, C:N ratios and del 13C isotope value for lakes and rivers on North Slope from Brooks Range to Prudhoe Bay, Arctic LTER 1988 to 2005
Investigator 1: 
First Name:George
Last Name:Kling
Address line 1:University of Michigan
Address line 2:Department of Ecology and Evolutionary Biology
Address line 3:830 North University
City:Ann Arbor
State:MI
Zip Code:48109-1048
Country:United States of America
Investigator 2: 
First Name:Chris
Last Name:Luecke
Address line 1:Department of Watershed Sciences
Address line 2:Utah State University
Address line 3:
City:Logan
State:Utah
Zip Code:84322-5210
Country:United States of America
Associate Investigators:Chris Wallace, Amanda Field, Greta Burkart
Keywords:isotope, C/N ratio, C:N, del15N, del13C, %C, %N, stable isotopes, DIC, POM, zooplankton, phytoplankton, seston, fish
Abstract:Composite file describing plant, animal, water, and sediment samples collected at various sites near Toolik Research Station (68 38'N, 149 36'W). Sample site descriptors include an assigned number specific to the file, a number that relates the samples to other samples collected on the same date and time (sortchem), site, date, time, and depth. Samples are identified by type, category, and a short description. Data include isotope values, carbon and nitrogen concentrations, and C:N ratios of samples.
Contact: Arctic LTER Information Manager
The Ecosystems Center
Marine Biological Lab
7 MBL St
Woods Hole, MA 02543
Phone (508) 289 7496
Email: arc_im@mbl.edu
Online URL: http://ecosystems.mbl.edu/ARC/
DATA FILE INFORMATION:
Data File URL http://ecosystems.mbl.edu/ARC/lakes/isotopes/data/1988-2005_Isotopes_Kling.dat
Data File Name 1988-2005_Isotopes_Kling.dat
Beginning Date 6/25/1998
End Date 8/24/2005
Number of Data Records 13424
Other Files to Reference
Availability Status
Quality Control Information
Maintenance Description
Log of Changes: Entered metadata into new metadata form.
RESEARCH LOCATION: Site 1
Geographic Description Toolik lake; lakes E5 and E6; Fog 2 and Fog 4; and lake N2. All lakes are located north/northeast of Toolik Lake and Toolik Lake Field Station.
Location Bounding Box
West Bounding Coordinate
East Bounding Coordinate
North Bounding Coordinate
South Bounding Coordinate
OR if single point location
Latitude 68.6256
Longitude -149.59605
Methods:For water sampling protocol, see the Land-Water protocol accessible from the Land-Water main page on the Arctic LTER web page or at http://www-personal.umich.edu/~gwk and clicking on the protocol link at the bottom of the page.

Specific methodologies are described in the protocol book listed above and a general description is provided below. See also,
Kling, G. W., B. Fry, and W. J. O'Brien. 1992. Stable isotopes and planktonic trophic structure in arctic lakes. Ecology 73:561-566.

All organic matter samples (sediments, algae, animals, etc.) were dried at temperatures less than 45 degC to prevent volatization.

Particulate organic carbon (POM) samples were collected from the water column by filtering as much sample water through a 25 or 45 mm Whatman GF/F filter as possible. Attached algae were collected from surfaces (sediments, rocks, plants), and aquatic macrophytes were collected using the most recent growth tissues.

Zooplankton species were identified and individuals were separated by hand, then grouped by species for isotopic determination. Other benthic or pelagic organisms were separated by hand to species or to the nearest taxonomic group. Species with carbonate shells were bathed in weak acid to remove inorganic C contamination before drying and analysis.

Samples for the determination of 13C-DIC were collected using serum vials following the DIC collection technique described in detail in the Kling laboratory protocol (Old DIC method). Samples are collected at depths representative of the epilimnion, metalimnion, and hypolimnion (if present) or from the epilimnion in shallow lakes. The depths were commonly 1 m, 3 - 5 m, and 8 - 9 m for lake E5 and 1 m for lake E6. The sampling depths are determined in the field and are based upon the depth profile of temperature and chlorophyll estimated by fluorescence using a Hydrolab multisensor datalogger. Samples were collected using a Van Dorn water sampler lowered to the specific depths and subsampled using large Nalgene bottles. A bubble-free subsample (no exposure to the atmosphere) was then over-filled (for flushing to remove bubbles) into 30 or 60 mL serum vials. A supersaturated solution of mercuric chloride was added (0.1 mL solution per 10 mL sample) to the sample to stop biological activity in the bottle and the vial was capped with an aluminum cap lined with a teflon septa.

Organic and sediment samples were analyzed mainly at the Marine Biological Laboratory (MBL) Stable Isotope Laboratory for 13C and 15N.(1)

13C-DIC samples were analyzed by the Stable Isotope Laboratory at the University of Michigan on a Finnigan MAT Delta S. (2)

(1) Marine Biological Laboratory (MBL) Stable Isotope Laboratory: http://ecosystems.mbl.edu/SILAB/default.html
The Ecosystems Center, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543 U.S.A.
(2) University of Michigan Stable Isotope Laboratory: http://www-personal.umich.edu/%7Eloraw/

Data Table

Variable Name Variable Description Units Measurement Scale Code Infomation Number Type DateTime Format Missing Value Code Missing Value Code Explanation
Isotope ID A unique number assigned to each sample within the Isotope file. This number is unrelated to the sortchem.   nominal       . .= Isotope ID was never assigned, or is unavailable
SortChem A unique number assigned to each sample collected at a specific site at a specific date and time. The year that the sample was collected is followed by a dash and then a sequential number. For example, if 600 samples were collected in 2002, then the first sample collected is 2002-0001 and the last sample is 2002-0600.   nominal       . Sortchem not used = unique number was never assigned to a sample.
Site Site Name. If a soil site, the site is described in the name by general location then specific feature (watertrack,etc) then specific point (1,2,3,...). If a lake or streams site, the lake or stream name (as it is commonly known) is given. Exceptions in naming procedure are provided in the Code Information and in AK-LTER_Site_Info.xls.   nominal TW=Tussock Watershed| WT=Watertrack| RE=Rain Event| I=I series lakes and inlets/outlets        
Date Sampling Date   datetime     dd-mmm-yy . .=not measured, written down in field, or not analyzed (sample lost,...)
Time_hr_dst Sampling Time in Alaska Daylight Savings Time (1 hour ahead of Alaska Standard Time: if 13:00 DST than 12:00 AST)   datetime     hh:mm . .=not measured, written down in field, or not analyzed (sample lost,...)
Depth_m Depth from surface of lake, stream, or soil. meter ratio   real   . .=not measured, written down in field, or not analyzed (sample lost,...)
Type General category describing the sample type.   nominal filter = filtered water sample| animal = animal tissue sample| plant = plant tissue sample| sediment = lake sediment sample     . .= unknown
Category Subcategory of "Type" category which further describes the sample.   nominal DOM = dissolved organic material| DIC = dissolved inorganic carbon.     . .= unknown
Description Description of sample, often giving taxonometric or filter information. This column contains the original information used to describe a sample.   nominal       . .= unknown
del15N_‰ Delta value of 15N with respect to air (standard) partsPerThousand ratio   real   . .= not measured, or lost.
N_umol_L Nitrogen concentration microMolesPerLiter ratio   real   . .= not measured, or lost.
N_%_Mass Percent nitrogen content by mass. percent ratio   real   . .= not measured, or lost.
del13C_‰ Delta value of 13C with respect to VPDB (Vienna Peedee Belemnite standard) partsPerThousand ratio   real   . .= not measured, or lost.
C_umol_L Concentration of carbon in sample microMolesPerLiter ratio   real   . .= not measured, or lost.
C_%_Mass Percent carbon content by mass percent ratio   real   . .= not measured, or lost.
Ratio_C_to_N Ratio of carbon to nitrogen by mass dimensionless ratio   real   . .= not measured, or lost.

Please contact arc_im@mbl.edu with questions, comments, or for technical assistance regarding this web site.