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Conditions of Use

The re-use of scientific data has the potential to greatly increase communication, collaboration and synthesis within and among disciplines, and thus is fostered, supported and encouraged. Permission to use this dataset is granted to the Data User free of charge subject to the following terms:

1) Acceptable use. Use of the dataset will be restricted to academic, research, educational, government, recreational, or other not-for-profit professional purposes. The Data User is permitted to produce and distribute derived works from this dataset provided that they are released under the same license terms as those accompanying this Data Set. Any other uses for the Data Set or its derived products will require explicit permission from the dataset owner.
2 ) Redistribution. The data are provided for use by the Data User. The metadata and this license must accompany all copies made and be available to all users of this Data Set. The Data User will not redistribute the original Data Set beyond this collaboration sphere.
3 ) Citation. It is considered a matter of professional ethics to acknowledge the work of other scientists. Thus, the Data User will properly cite the Data Set in any publications or in the metadata of any derived data products that were produced using the Data Set. Citation should take the following general form: Creator, Year of Data Publication, Title of Dataset, Publisher, Dataset identifier. For example:

Shaver, G. 1989. Above ground biomass in acidic tussock tundra experimental site, 1989, Arctic LTER, Toolik, Alaska. Arctic LTER, Marine Biological Lab, Woods Hole, Ma 02543. 1989gsttbm 

4 ) Acknowledgement. The Data User should acknowledge any institutional support or specific funding awards referenced in the metadata accompanying this dataset in any publications where the Data Set contributed significantly to its content. Acknowledgements should identify the supporting party, the party that received the support, and any identifying information such as grant numbers. For example:

Data sets were provided by the Arctic LTER. This material is based upon work supported by the National Science Foundation under Grants #DEB-981022, 9211775, 8702328; #OPP-9911278, 9911681, 9732281, 9615411, 9615563, 9615942, 9615949, 9400722, 9415411, 9318529; #BSR 9019055, 8806635, 8507493.

5 ) Notification. The Data User will notify the Data Set Contact when any derivative work or publication based on or derived from the Data Set is distributed. The Data User will provide the data contact with two reprints of any publications resulting from use of the Data Set and will provide copies, or on-line access to, any derived digital products. Notification will include an explanation of how the Data Set was used to produce the derived work.
6 ) Collaboration. The Data Set has been released in the spirit of open scientific collaboration. Data Users are thus strongly encouraged to consider consultation, collaboration and/or co-authorship with the Data Set Creator.

By accepting this Data Set, the Data User agrees to abide by the terms of this agreement. The Data Owner shall have the right to terminate this agreement immediately by written notice upon the Data User's breach of, or non-compliance with, any of its terms. The Data User may be held responsible for any misuse that is caused or encouraged by the Data User's failure to abide by the terms of this agreement.


While substantial efforts are made to ensure the accuracy of data and documentation contained in this Data Set, complete accuracy of data and metadata cannot be guaranteed. All data and metadata are made available "as is". The Data User holds all parties involved in the production or distribution of the Data Set harmless for damages resulting from its use or interpretation.

Dataset URLs:METADATA: HTML, Rich Text, XML(EML compliant)
DATA: Comma Delimited, Excel file with Metadata and data, Dataset via LTER Data Poral
Dataset ID:1988-2005_Isotopes_Kling.04
Dataset Title:Concentration of dissolved inorganic carbon (DIC), carbon and nitrogen concentrations, C:N ratios and del 13C isotope value for lakes and rivers on North Slope from Brooks Range to Prudhoe Bay, Arctic LTER 1988 to 2005
Investigator 1: 
First Name:George
Last Name:Kling
Organization:University of Michigan
Address line 2:Department of Ecology and Evolutionary Biology
Address line 3:830 North University
City:Ann Arbor
Zip Code:48109-1048
Country:United States of America
Investigator 2: 
First Name:Chris
Last Name:Luecke
Organization:Department of Watershed Sciences
Address line 2:Utah State University
Address line 3:
Zip Code:84322-5210
Country:United States of America
Investigator 3: 
First Name:Chris
Last Name:Wallace
Address line 2:
Zip Code:
Investigator 4: 
First Name:Amanda
Last Name:Field
Investigator 5: 
First Name:Greta
Last Name:Burkart
Associate Investigators:
Keywords:isotopes, carbon to nitrogen ratio, del 15N, del 13C, percent carbon, percent nitrogen, stable isotopes, dissolved inorganic carbon, particulate organic matter, zooplankton, phytoplankton, seston, fishes, arctic lakes, lakes
Abstract:Composite file describing plant, animal, water, and sediment samples collected at various sites near Toolik Research Station (68 38'N, 149 36'W). Sample site descriptors include an assigned number specific to the file, a number that relates the samples to other samples collected on the same date and time (sortchem), site, date, time, and depth. Samples are identified by type, category, and a short description. Data include isotope values, carbon and nitrogen concentrations, and C:N ratios of samples.
For questions about the Metadata and data contact the Investigators.
For information about this web site contact:
Arctic LTER Information Manager
The Ecosystems Center
Marine Biological Lab
7 MBL St
Woods Hole, MA 02543
Phone (508) 289 7496
Online URL:
Data File URL
Data File Name 1988-2005_Isotopes_Kling.dat
Beginning Date 6/25/1998
End Date 8/24/2005
Number of Data Records 838
Other Files to Reference
Availability Status
Quality Control Information
Maintenance Description
Log of Changes: Entered metadata into new metadata form.
Version 3: Updated the metadata form to include the sites and to version emal 2.1 JimL 24May13
Version 4: Checked keywords against the LTER network preferred list and replaced non-preferred terms. Jim L 15Jan14
RESEARCH LOCATION:                  
Location Name E 05 E 06 Fog Lake 2 Fog Lake 4 Toolik N 02 Select Site or enter New One Select Site or enter New One  
Geographic Description E 05 E 06 Fog Lake 2 Fog Lake 4 Toolik Lake Main Station Lake N2 Enter Description Enter Description  
Location Bounding Box                  
West Bounding Coordinate                  
East Bounding Coordinate                  
North Bounding Coordinate                  
South Bounding Coordinate                  
OR if single point location                  
Latitude 68.6256 68.6434270583 68.6833333333333 68.68 68.629961 68.6409472345 In Decimal Degrees In Decimal Degrees  
Longitude -149.59605 -149.440564592 -149.1 -149.071666666667 -149.612633 -149.625086307 In Decimal Degrees In Decimal Degrees  
Elevation 719 792 785 754 719 724 In Meters In Meters  
Link to Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map      
Organisms studied
Methods:For water sampling protocol, see the Land-Water protocol accessible from the Land-Water main page on the Arctic LTER web page or at and clicking on the protocol link at the bottom of the page.

Specific methodologies are described in the protocol book listed above and a general description is provided below. See also,
Kling, G. W., B. Fry, and W. J. O'Brien. 1992. Stable isotopes and planktonic trophic structure in arctic lakes. Ecology 73:561-566.

All organic matter samples (sediments, algae, animals, etc.) were dried at temperatures less than 45 degC to prevent volatization.

Particulate organic carbon (POM) samples were collected from the water column by filtering as much sample water through a 25 or 45 mm Whatman GF/F filter as possible. Attached algae were collected from surfaces (sediments, rocks, plants), and aquatic macrophytes were collected using the most recent growth tissues.

Zooplankton species were identified and individuals were separated by hand, then grouped by species for isotopic determination. Other benthic or pelagic organisms were separated by hand to species or to the nearest taxonomic group. Species with carbonate shells were bathed in weak acid to remove inorganic C contamination before drying and analysis.

Samples for the determination of 13C-DIC were collected using serum vials following the DIC collection technique described in detail in the Kling laboratory protocol (Old DIC method). Samples are collected at depths representative of the epilimnion, metalimnion, and hypolimnion (if present) or from the epilimnion in shallow lakes. The depths were commonly 1 m, 3 - 5 m, and 8 - 9 m for lake E5 and 1 m for lake E6. The sampling depths are determined in the field and are based upon the depth profile of temperature and chlorophyll estimated by fluorescence using a Hydrolab multisensor datalogger. Samples were collected using a Van Dorn water sampler lowered to the specific depths and subsampled using large Nalgene bottles. A bubble-free subsample (no exposure to the atmosphere) was then over-filled (for flushing to remove bubbles) into 30 or 60 mL serum vials. A supersaturated solution of mercuric chloride was added (0.1 mL solution per 10 mL sample) to the sample to stop biological activity in the bottle and the vial was capped with an aluminum cap lined with a teflon septa.

Organic and sediment samples were analyzed mainly at the Marine Biological Laboratory (MBL) Stable Isotope Laboratory for 13C and 15N.(1)

13C-DIC samples were analyzed by the Stable Isotope Laboratory at the University of Michigan on a Finnigan MAT Delta S. (2)

(1) Marine Biological Laboratory (MBL) Stable Isotope Laboratory:
The Ecosystems Center, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543 U.S.A.
(2) University of Michigan Stable Isotope Laboratory:

Data Table

Variable Name Variable Description Data Type Units DateTime Format Code Information Missing Value Code
Isotope ID A unique number assigned to each sample within the Isotope file. This number is unrelated to the sortchem. text       .=Missing or Not Measured
SortChem A unique number assigned to each sample collected at a specific site at a specific date and time. The year that the sample was collected is followed by a dash and then a sequential number. For example, if 600 samples were collected in 2002, then the first sample collected is 2002-0001 and the last sample is 2002-0600. text       .=Missing or Not Measured
Site Site Name. If a soil site, the site is described in the name by general location then specific feature (watertrack,etc) then specific point (1,2,3,...). If a lake or streams site, the lake or stream name (as it is commonly known) is given. Exceptions in naming procedure are provided in the Code Information and in AK-LTER_Site_Info.xls. text        
Date Sampling Date datetime   dd-mmm-yyyy   .=Missing or Not Measured
Time_hr_dst Sampling Time in Alaska Daylight Savings Time (1 hour ahead of Alaska Standard Time: if 13:00 DST than 12:00 AST) datetime   hh:mm   .=Missing or Not Measured
Depth_m Depth from surface of lake, stream, or soil. In meters or lake zone. text       .=Missing or Not Measured
Type General category describing the sample type. text       .=Missing or Not Measured
Category Subcategory of "Type" category which further describes the sample. text       .=Missing or Not Measured
Description Description of sample, often giving taxonometric or filter information. This column contains the original information used to describe a sample. text       .=Missing or Not Measured
del15N_ Delta value of 15N with respect to air (standard) number partPerThousand     .=Missing or Not Measured
N_umol_L Nitrogen concentration number micromolePerLiter     .=Missing or Not Measured
N_%_Mass Percent nitrogen content by mass. number percent     .=Missing or Not Measured
del13C_ Delta value of 13C with respect to VPDB (Vienna Peedee Belemnite standard) number partPerThousand     .=Missing or Not Measured
C_umol_L Concentration of carbon in sample number micromolePerLiter     .=Missing or Not Measured
C_%_Mass Percent carbon content by mass number percent     .=Missing or Not Measured
Ratio_C_to_N Ratio of carbon to nitrogen by mass number dimensionless     .=Missing or Not Measured

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