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Conditions of Use

The re-use of scientific data has the potential to greatly increase communication, collaboration and synthesis within and among disciplines, and thus is fostered, supported and encouraged. Permission to use this dataset is granted to the Data User free of charge subject to the following terms:

1) Acceptable use. Use of the dataset will be restricted to academic, research, educational, government, recreational, or other not-for-profit professional purposes. The Data User is permitted to produce and distribute derived works from this dataset provided that they are released under the same license terms as those accompanying this Data Set. Any other uses for the Data Set or its derived products will require explicit permission from the dataset owner.
2 ) Redistribution. The data are provided for use by the Data User. The metadata and this license must accompany all copies made and be available to all users of this Data Set. The Data User will not redistribute the original Data Set beyond this collaboration sphere.
3 ) Citation. It is considered a matter of professional ethics to acknowledge the work of other scientists. Thus, the Data User will properly cite the Data Set in any publications or in the metadata of any derived data products that were produced using the Data Set. Citation should take the following general form: Creator, Year of Data Publication, Title of Dataset, Publisher, Dataset identifier. For example:

Shaver, G. 1989. Above ground biomass in acidic tussock tundra experimental site, 1989, Arctic LTER, Toolik, Alaska. Arctic LTER, Marine Biological Lab, Woods Hole, Ma 02543. 1989gsttbm 

4 ) Acknowledgement. The Data User should acknowledge any institutional support or specific funding awards referenced in the metadata accompanying this dataset in any publications where the Data Set contributed significantly to its content. Acknowledgements should identify the supporting party, the party that received the support, and any identifying information such as grant numbers. For example:

Data sets were provided by the Arctic LTER. This material is based upon work supported by the National Science Foundation under Grants #DEB-981022, 9211775, 8702328; #OPP-9911278, 9911681, 9732281, 9615411, 9615563, 9615942, 9615949, 9400722, 9415411, 9318529; #BSR 9019055, 8806635, 8507493.

5 ) Notification. The Data User will notify the Data Set Contact when any derivative work or publication based on or derived from the Data Set is distributed. The Data User will provide the data contact with two reprints of any publications resulting from use of the Data Set and will provide copies, or on-line access to, any derived digital products. Notification will include an explanation of how the Data Set was used to produce the derived work.
6 ) Collaboration. The Data Set has been released in the spirit of open scientific collaboration. Data Users are thus strongly encouraged to consider consultation, collaboration and/or co-authorship with the Data Set Creator.

By accepting this Data Set, the Data User agrees to abide by the terms of this agreement. The Data Owner shall have the right to terminate this agreement immediately by written notice upon the Data User's breach of, or non-compliance with, any of its terms. The Data User may be held responsible for any misuse that is caused or encouraged by the Data User's failure to abide by the terms of this agreement.


While substantial efforts are made to ensure the accuracy of data and documentation contained in this Data Set, complete accuracy of data and metadata cannot be guaranteed. All data and metadata are made available "as is". The Data User holds all parties involved in the production or distribution of the Data Set harmless for damages resulting from its use or interpretation.

Dataset URLs:METADATA: HTML, Rich Text, XML(EML compliant)
DATA: Comma Delimited, Excel file with Metadata and data, Dataset via LTER Data Poral
Dataset ID:2005_bacprods_Kling.09
Dataset Title:Bacterial Production Data for lakes and lake inlets/outlets samples collected summer 2005, Arctic LTER, Toolik Research Station, Alaska.
Investigator 1: 
First Name:George
Last Name:Kling
Organization:University of Michigan
Address line 2:Department of Ecology and Evolutionary Biology
Address line 3:830 North University
City:Ann Arbor
Zip Code:48109-1048
Country:United States of America
Investigator 2: 
First Name:Amanda
Last Name:Field
Address line 2:
Address line 3:
Zip Code:
Investigator 3: 
First Name:Heather
Last Name:Adams
Investigator 4: 
First Name:Kristi
Last Name:Judd
Associate Investigators:
Keywords:bacteria, transplant, communities, lakes, streams, inlet stream, bacterial production, carbon, carbon assimilation, 14C-leucine, 14C, populations, arctic lakes, arctic streams
Abstract:Yearly file containing information on bacterial productivity. Samples were collected at various sites near Toolik Research Station (68 38'N, 149 36'W). Sample site descriptors include an assigned number (sortchem), site, date, time and depth.
For questions about the Metadata and data contact the Investigators.
For information about this web site contact:
Arctic LTER Information Manager
The Ecosystems Center
Marine Biological Lab
7 MBL St
Woods Hole, MA 02543
Phone (508) 289 7496
Online URL:
Data File URL
Data File Name 2005_bacprods_Kling
Beginning Date 6/21/2005
End Date 8/18/2005
Number of Data Records 168
Other Files to Reference
Availability Status
Quality Control Information
Maintenance Description
Log of Changes: Entered metadata into new metadata form.
ver2 Corrected EML lat lon
Version 3: Data URL was changed to point to the LTERNET Data Access server
Version 4: LTERNET Data Access server proxy changed from to
Version 5: LTERNET Data Access server proxy changed from to
Version 7: Update LTERNET Data Access server proxy link for Excel and comma delimited data files. Changed from knb to das in url.
Version 8: Updated metadata form to newer version (without site sheet). Fixed discrepency with header variable names. CH 1Feb2013. Updated metadata to newer version (with sites sheet) CH March 2013. Time format updated, new urls and eml files for web created JD-Nov2013
Version 9: Checked keywords against the LTER network preferred list and replaced non-preferred terms. Jim L 15Jan14
RESEARCH LOCATION:                                                          
Location Name Toolik Toolik central Toolik inlet I1 I2 I3 I4 I5 I6 I7 I8 I1 into I3 I2 into I3 I4 into I5 I6 headwater lake I swamp I swamp inlet I6 headwater lake inlet I6 headwater lake outlet I5 into I6 I5 outlet I6 inlet west I7 into I9 I8 into I9 I8 inlet I8 outlet I8 headwater Milkyway lower  
Geographic Description Toolik Lake and vicinity. See Toolik central Toolik inlet I1 I2 I3 I4 I5 I6 I7 I8 I1 into I3 I2 into I3 I4 into I5 I6 headwater lake I swamp I swamp inlet I6 headwater lake inlet I6 headwater lake outlet I5 into I6 I5 outlet I6 inlet west I7 into I9 I8 into I9 I8 inlet I8 outlet I8 headwater Milkyway lower  
Location Bounding Box                                                          
West Bounding Coordinate                                                          
East Bounding Coordinate                                                          
North Bounding Coordinate                                                          
South Bounding Coordinate                                                          
OR if single point location                                                          
Latitude 68.6256 68.632586 68.6256 68.5687130789 68.5713195633 68.5755366301 68.57956715 68.5873874391 68.5965924039 68.6008747982 68.6101575207 68.574 68.57478333 68.58423333 68.582062 68.610781 68.61035 68.578643 68.58357 68.59491667 68.589087 68.59688333 68.61818333 68.61838333 68.60853333 68.6114075339 68.5736666666667 68.622  
Longitude -149.59605 -149.600895 -149.59605 -149.58807625 -149.565881618 -149.583644456 -149.58405938 -149.589625877 -149.59264335 -149.596582063 -149.582211513 -149.583566 -149.58205 -149.5836 -149.622932 -149.600742 -149.599766 -149.621102 -149.62384 -149.586316 -149.589219 -149.60125 -149.596766 -149.5965 -149.587633 -149.590131872 -149.537166666667 -149.590666  
Elevation 719 719 719 785 785 774 770 767 754 742 744 774 774 770 806 736 736 808 808 754 767 754 728 728 744 744 808 725  
Link to Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map  
Organisms studied
Methods:For the bacterial production protocol, see the Land-Water protocol accessible from the Land-Water mainpage on the Arctic LTER web page or at and clicking on the protocol link at the bottom of the page.

Bacterial production is determined by the incorporation of 13C labeled Leucine (Simon and Azam 1989). Water samples are vacuum filtered through 0.22 um Millapore GS cellulose nitrate filters. Samples are extracted with 5mL of 5% TCA for 5 minutes, rinsed with additional TCA, and air dried in mini-scintillation vials. Following the addition of 1 mL cellusolve, filters are left to dissolve overnight. Scinti-safe scintillation cocktail (4 mL) is then added to each vial and samples are counted at Toolik Field Station on a liquid scintillation counter (Packard Tri-Carb 2100TR) .

Calculations of bacterial productivity assume that the Internal Dilution (ID) of leucine is 1, but studies show it may range from 1-2 (Kirchman 1993). Some published data from these LTER files have used an ID value different than 1; in those studies, the ID value used is stated in the article.

1. Simon, M., and F. Azam. 1989. Protein content and protein synthesis rates of planktonic marine bacteria. Marine Ecology Progress Series 51:201-213.
2. Kirchmann, D.L. 1993. Leucine incorporation as a measure of biomass production by heterotrophic bacteria. Pages 509-512 in P.F. Kemp, B.F. Sherr, E.B. Sherr, and J.J. Cole, editors. Handbook of methods in aquatic microbial ecology. Lewis Publishers, Boca Raton, Florida, USA.

Data Table

Variable Name Variable Description Data Type Units DateTime Format Code Information Missing Value Code
SortChem Yearly number assigned to samples collected at a specific site at a specific date and time. The year that the sample was collected is followed by a dash and then a sequential number. For example, if 600 samples were collected in 2002, then the first sample collected is 2002-0001 and the last sample is 2002-0600. text        
Site Site Name. If a soil site, the site is described in the name by general location then specific feature (watertrack,etc) then specific point (1,2,3,...). If a lake or streams site, the lake or stream name (as it is commonly known) is given. Exceptions in naming procedure are provided in the Code Information. text        
Date Sampling Date datetime   dd-mmm-yy   .=Missing or Not Measured
Time_hr_dst Sampling Time in Alaska Daylight Savings Time (1 hour ahead of Alaska Standard Time: if 13:00 DST than 12:00 AST) datetime   hh:mm   .=Missing or Not Measured
Depth_m Depth from surface of lake, stream, or soil number meter     .=Missing or Not Measured
Bac_prod_ug C_L_day Bacterial Productivity number microgramPerLiterPerDay     .=Missing or Not Measured

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