Arctic LTER Database

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The re-use of scientific data has the potential to greatly increase communication, collaboration and synthesis within and among disciplines, and thus is fostered, supported and encouraged. Permission to use this dataset is granted to the Data User free of charge subject to the following terms:

1) Acceptable use. Use of the dataset will be restricted to academic, research, educational, government, recreational, or other not-for-profit professional purposes. The Data User is permitted to produce and distribute derived works from this dataset provided that they are released under the same license terms as those accompanying this Data Set. Any other uses for the Data Set or its derived products will require explicit permission from the dataset owner.
2 ) Redistribution. The data are provided for use by the Data User. The metadata and this license must accompany all copies made and be available to all users of this Data Set. The Data User will not redistribute the original Data Set beyond this collaboration sphere.
3 ) Citation. It is considered a matter of professional ethics to acknowledge the work of other scientists. Thus, the Data User will properly cite the Data Set in any publications or in the metadata of any derived data products that were produced using the Data Set. Citation should take the following general form: Creator, Year of Data Publication, Title of Dataset, Publisher, Dataset identifier. For example:

Shaver, G. 1989. Above ground biomass in acidic tussock tundra experimental site, 1989, Arctic LTER, Toolik, Alaska. Arctic LTER, Marine Biological Lab, Woods Hole, Ma 02543. 1989gsttbm http://ecosystems.mbl.edu/arc/terrest/biomass/index.shtml 

4 ) Acknowledgement. The Data User should acknowledge any institutional support or specific funding awards referenced in the metadata accompanying this dataset in any publications where the Data Set contributed significantly to its content. Acknowledgements should identify the supporting party, the party that received the support, and any identifying information such as grant numbers. For example:

Data sets were provided by the Arctic LTER. This material is based upon work supported by the National Science Foundation under Grants #DEB-981022, 9211775, 8702328; #OPP-9911278, 9911681, 9732281, 9615411, 9615563, 9615942, 9615949, 9400722, 9415411, 9318529; #BSR 9019055, 8806635, 8507493.

5 ) Notification. The Data User will notify the Data Set Contact when any derivative work or publication based on or derived from the Data Set is distributed. The Data User will provide the data contact with two reprints of any publications resulting from use of the Data Set and will provide copies, or on-line access to, any derived digital products. Notification will include an explanation of how the Data Set was used to produce the derived work.
6 ) Collaboration. The Data Set has been released in the spirit of open scientific collaboration. Data Users are thus strongly encouraged to consider consultation, collaboration and/or co-authorship with the Data Set Creator.

By accepting this Data Set, the Data User agrees to abide by the terms of this agreement. The Data Owner shall have the right to terminate this agreement immediately by written notice upon the Data User's breach of, or non-compliance with, any of its terms. The Data User may be held responsible for any misuse that is caused or encouraged by the Data User's failure to abide by the terms of this agreement.

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While substantial efforts are made to ensure the accuracy of data and documentation contained in this Data Set, complete accuracy of data and metadata cannot be guaranteed. All data and metadata are made available "as is". The Data User holds all parties involved in the production or distribution of the Data Set harmless for damages resulting from its use or interpretation.

Dataset URLs:	METADATA: HTML, Rich Text, XML(EML compliant) DATA: Comma Delimited, Excel file with Metadata and data
Dataset ID:	1990_current_peochl
Dataset Title:	Epilithic and sestonic chlorophyll for Oksrukuyik Creek during the summers of 1990-2009
Investigator 1:
First Name:	Bruce
Last Name:	Peterson
Address line 1:	Marine Biological Lab
Address line 2:	7 MBL St.
Address line 3:
City:	Woods Hole
State:	MA
Zip Code:	02543
Country:	USA
Investigator 2:
First Name:	Breck
Last Name:	Bowden
Address line 1:	University of Vermont
Address line 2:	304 Aiken Center
City:	Burlington
State:	VT
Zip Code:	05405
Country:	USA
Associate Investigators:	Elissa Schuett, Adrian Green, Karie Slavik
Keywords:	Chlorophyll a, Epilithic Algae, sestonic, Oksrukuyik Creek, primary production, disturbance
Abstract:	Sestonic and epilithic chlorophyll for the Oksrukuyik Creek during the summers of 1990 - 2009. Water was filtered and rocks from designated locations on the Oksrukuyik Creek were scrubbed for epilithic algae approximately three times each summer. Sampling formerly included stations in the fertilized and reference reaches. Current sampling stations are in the reference reach of the stream, upstream of the point of phosphorus addition that occurred from 1991-1996. Sampling dates are typically once in each June, July, and August.
Contact:	Arctic LTER Information Manager The Ecosystems Center Marine Biological Lab 7 MBL St Woods Hole, MA 02543 Phone (508) 289 7496 Email: arc_im@mbl.edu Online URL: http://ecosystems.mbl.edu/ARC/
DATA FILE INFORMATION:
Data File URL	http://metacat.lternet.edu/das/dataAccessServlet?docid=knb-lter-arc.10016&urlTail=streams/primprod/data/1990_current_peochl.dat
Data File Name	1990_current_peochl.dat
Beginning Date	6/1/1990
End Date	9/1/2009
Number of Data Records	2262
Other Files to Reference
Availability Status	Type 1
Quality Control Information
Maintenance Description	data updated each year, ongoing data collection
Log of Changes:	File created 23Apr08 by EBS with 2007 data, metadata
	Version 2: Added LTERNET Data Access server proxy for Excel and comma delimited data files.
	Version 3: added 2004-2006, 2008-2009 data, updated metadata. 28Oct09 EBS
	Version 4: added sestonic data and old data, updated metadata 15Jan10 EBS
	Version 4: Update LTERNET Data Access server proxy link for Excel and comma delimited data files. Changed from knb to das in url.

RESEARCH LOCATION:	Site 1	Site 2
Geographic Description	Oksrukuyik Creek, North Slope of Alaska, reference reach and formerly fertilized reach. Distances are based on original 1992 phosphorus dripper and are in stream kilometers. Upstream stations are negative, downstream are positive.
Location Bounding Box
West Bounding Coordinate
East Bounding Coordinate
North Bounding Coordinate
South Bounding Coordinate
OR if single point location
Latitude	68.6863
Longitude	-149.0973
Elevation

TAXONOMIC COVERAGE:
Taxonomic Protocols
Organisms studied
Methods:	Sestonic chlorophyll a was sampled by filtering 500 mL stream water through a 47mm filter. The fitlers were returned to the lab and placed in 10ml of 90% buffered acetone and extracted for 24 hours. The samples were then read on a Turner 111 (1990-1991) and Turner 450 (1992-1997), TurnerDesigns 10AU (1998-2009) Fluorometers. Epilithic chlorophyll was sampled using either a "2x2" or "whole rock" scrubbing method. 2x2 method: Epilithic chlorophyll was sampled three times on rocks from riffles. Using a plastic 35mm slide mount to partition a known area, the algae was scraped from the rock surface with a brass brush. The brushed area of the rock, the brush and slide holder were then rinsed and the slurry collected and brought up to a volume of 56 ml. Five rocks were sampled from each riffle station. Whole rock method: Whole rock samples were also collected from riffles. The upper surface of rocks fitting into an area of 546cm^2 ( +/- 18cm) (363 cm2 ( +/- 18cm) in 1996) were scrubbed with a brass brush. The slurry was brought to a volume of 1 L.The samples were returned to the lab where they were filtered through a GF/C glass fiber filter. The filters were then placed in 10 ml of 90% acetone and extracted in the dark for 24 hours. The samples were then read on a Turner 111 (1990-1991) and Turner 450 (1992-1997), TurnerDesigns 10AU (1998-2009) Fluorometers. Reference Citations: R.G. Wetzel and G.E. Likens. 1979. Limnological Methods. Saunders. Philadelphia. Notes: In 2004 several adjustments were made to the protocol from 2003. Extraction took place at approximately 4C using chilled acetone. A multipoint curve was analyzed using a chl a stock std. A Turner Designs solid standard could then be utilized as a check. GF/F 25mm filters were used. Additionally, chl a was corrected for pheophytin following EPA 445 and data is reported in corrected chl a. For more information please reference the chlorophyll protocol available on the Arctic LTER website. http://ecosystems.mbl.edu/arc/data_doc/lakes/cpcpractice/LakesChemistryProtocolsmain.htm

Data Table

Variable Name	Variable Description	Units	Measurement Scale	Code Information	Number Type	DateTime Format	Missing Value Code	Missing Value Code Explanation
SITE CODE	LTER Site code		nominal
Year	Year of sample		datetime			yyyy
Type	Type of sample (e.g. sestonic, whole rock epilithic, 2x2 epilithic)		nominal
Date	Date		datetime	protocol used		dd-mmm-yyyy
D/D	Distance from P dripper, usually in kilometers. Negative numbers indicate upstream from fertilization; positive numbers are downstream		nominal
Chl a (ug/cm2)	Phaeophyton corrected Chl a (ug/cm^2) for whole rock scrubs	microgramPerCentimeterSquared	ratio		real		-9999	sample contaminated
Chl a (ug/L)	Phaeophyton corrected Chl a (ug/cm^2) for sestonic	microgramPerLiter	ratio		real		-9999	sample contaminated
	Please contact arc_im@mbl.edu with questions, comments, or for technical assistance regarding this web site.