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Arctic LTER Database

Acceptance and utilization of LTER data requires that:

(1) The Principal Investigator be sent a notice stating reasons for acquiring any data and a description of the publication intentions.
(2) The Principal Investigator of the data set be sent a copy of the report or manuscript prior to submission and be adequately cited in any resultant publications.
(3) A copy of any resultant publications should be sent to:

Principal Investigator
Ecosystems Center
Marine Biological Laboratory
7 MBL St.
Woods Hole, MA 02543

Older Metadata: Attribute table not in XML (EML) file

Dataset URLs:	METADATA: HTML, Rich Text, XML(EML compliant) DATA: Comma Delimited, Excel file with Metadata and data
Dataset ID:	96bomoct
Dataset Title:	Chlorophyll concentrations of Schistidium agassizii and Hygrohypnum spp, Arctic LTER 1996.
Investigator 1:
First Name:	William
Last Name:	Bowden
Address line 1:
Address line 2:
Address line 3:
City:
State:
Zip Code:
Country:
Associate Investigators:	Dave Arscott, Jim Lee
Keywords:	chlorophyll, Kuparuk River, reference, recovery, fertilized, bryophytes, riffle, Hygrohypnum, Schistidium
Abstract:	Chlorophyll concentrations of Schistidium agassizii and Hygrohypnum spp. retrieved from the Kuparuk River. In 1996, weekly, from 22-Jun to 10-Aug, collections of bryophytes from 3 riffles each treatment reach (i.e. reference, recovery, and fertilized).
Contact:	Arctic LTER Information Manager The Ecosystems Center Marine Biological Lab 7 MBL St Woods Hole, MA 02543 Phone (508) 289 7496 Email: arc_im@mbl.edu Online URL: http://ecosystems.mbl.edu/ARC/
DATA FILE INFORMATION:
Data File Name	96bomoct
Beginning Date	5/1/1996
End Date	9/1/1996
Number of Data Records
Other Files to Reference
Availability Status
Quality Control Information
Maintenance Description
Log of Changes:	Log of changes: For Archival Use: DATE RECEIVED: June 1997 DATA FILE ENTERED BY: Karie Slavik DATA FILE VALIDATION: NAME: DATE



RESEARCH LOCATION:
Geographic Description	Kuparuk River: Reference: riffles from 0.56 to 0.0 km Recovery: riffles from 0.59 to 1.3 km Fertilized: riffles from 0.6 to 7.5 km
Location Bounding Box
West Bounding Coordinate
East Bounding Coordinate
North Bounding Coordinate
South Bounding Coordinate
OR if single point location
Latitude
Longitude



TAXONOMIC COVERAGE:
Organisms studied

Methods:	In 1996, we moved the Kuparuk River's point of phosphorus addition 0.75 km downstream to stream benchmark +1.4 km. This created 3 treatment reaches: a reference reach (upstream of +0.59 km), a P-recovery reach (+0.59 to +1.35 km), and a long-term P-fertilized reach (1.35 to 10 km). Further downstream (typically > 10 km) nutrient conditions return to background concentrations (Peterson et al. 1993, Bowden et al. 1994). From each of these treatment reaches we have chosen 3 riffles from which to sample. For 8 consecutive weeks during the 1996 field season, total chlorophyll content of S. agassizii and Hygrohypnum spp. was determined. Each week collections of S. agassizii were retrieved from 3 riffles in each treatment reach and collections of Hygrohypnum spp. were retrieved from each of the designated 3 riffles in the recovery and fertilized reaches. Hygrohypnum spp. is rarely found in the reference zone and was therefore not included. Two, small, thumb-sized clumps of each species were randomly collected from each of the study riffles, rinsed to remove loose epiphytes, detritus, and invertebrates, and placed in a whirl-pak bag and transported to the laboratory. Because S. agassizii can be found on rocks that are more frequently exposed to the atmosphere than Hygrohypnum spp. an effort was made to collect clumps from permanently submerged substrate. At the laboratory 1 frond from each of the collected clumps was randomly selected for chlorophyll extraction. Each frond was patted dry and weighed on a Cahn C- 33 microbalance to record the fresh mass and then placed in 10 ml of 100 % DMSO for 40-48 hours for chlorophyll extraction (Alpert 1984, Hains 1985, Spencer and Ksander 1987). Chlorophyll was determined spectrophotometrically following methods and equations of Golterman (1969). Absorbency was read at 665 nm and (to correct for turbidity) at 750 nm. Samples were not acidified and thus, measurements represent total chlorophyll content. Ten additional fronds from each species were selected to develop a wet weight to dry weight relationship. The fronds ranged in size from small to large and each frond was weighed separately. After weighing, all fronds were dried at 65.0 oC to a consistent weight and re-weighed individually. Regression analysis was used to quantify the wet to dry relationship for each species. This relationship was established weekly for both species. Total chlorophyll concentrations were converted to dry mass specific estimates using the established wet to dry weight relationship and the equation is described below (Calculations). Calculations: Cd * Vd ::::- = CHLt Eq. 1 m where: CHLt = moss total chlorophyll (mg CHLt / gdw) Cd = chlorophyll concentration in DMSO (mg CHLt / L) Vd = volume of DMSO (0.01 L) m = dry mass from wet dry relationship (g) Reference Citations: Arscott, D. B. 1997. Primary Production in an Arctic Stream Ecosystem. Thesis, University of New Hampshire. Manuscript submitted. 1997. Arscott, Dave B., W. B. Bowden, and J. C. Finlay. Epilithic algal and bryophyte metabolism in an arctic tundra stream, Alaska. Manuscript submitted. 1997. Arscott, Dave B., W. B. Bowden, J. C. Finlay, and J. Lee. Ecophysiology of two arctic stream bryophytes. Alpert, P. 1984. Analysis of chlorophyll content in mosses through extraction in DMSO. The Bryologist 87(4):363-365. Bowden, W. B., J. C. Finlay, and P. E. Maloney. 1994. Long-term effects of PO4 fertilization on the distribution of bryophytes in an arctic river. Freshwater Biology 32:445-454. Bowden, W.B., B.J. Peterson, J.C. Finlay, and J. Tucker. 1992. Epilithic chlorophyll a, photosynthesis, and respiration in control and fertilized reaches of a tundra stream. Hydrobiologia 240: 121-131. Finlay, J. C. and W. B. Bowden. 1994. Controls on production of bryophytes in an arctic tundra stream. Freshwater Biology 32:455-466. Golterman, 1969. Studies on the cycle of elements in fresh water. Acta Botanica Neerlandica 9:1-58. Hains, J. J. 1985. Practical considerations for routine chlorophyll measurements: Precautions and comparison of extraction methods. Journal of Freshwater Ecology 3(2):175-179. Spencer, D. F., G. G. Ksander. 1987. Comparison of three methods for extracting chlorophyll from aquatic macrophytes. Journal of Freshwater Ecology 4(2):201-208. Sampling Description. None

Data Table

(11) VARIABLE DESCRIPTIONS: 
								Precise			Coded 
Variable			Variable description			Units		
	(Y/N) 
SITE          		site of measurement                          				y 
D/D           		distance from K or O dripper  		0.05 km        	
	n 
DATE          		date                          			ddmmyy         		n 
LOCATION      		location (REF, RECO, FERT)                   			
	n 
SUBSITE       		typically POOL or RIFFLE                     			
	n 
TYPE          		community description                        			
	n 
CHLT          		total chlorophyll content     		mgCHLt/gdw     	
	n