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The re-use of scientific data has the potential to greatly increase communication, collaboration and synthesis within and among disciplines, and thus is fostered, supported and encouraged. Permission to use this dataset is granted to the Data User free of charge subject to the following terms:

1) Acceptable use. Use of the dataset will be restricted to academic, research, educational, government, recreational, or other not-for-profit professional purposes. The Data User is permitted to produce and distribute derived works from this dataset provided that they are released under the same license terms as those accompanying this Data Set. Any other uses for the Data Set or its derived products will require explicit permission from the dataset owner.
2 ) Redistribution. The data are provided for use by the Data User. The metadata and this license must accompany all copies made and be available to all users of this Data Set. The Data User will not redistribute the original Data Set beyond this collaboration sphere.
3 ) Citation. It is considered a matter of professional ethics to acknowledge the work of other scientists. Thus, the Data User will properly cite the Data Set in any publications or in the metadata of any derived data products that were produced using the Data Set. Citation should take the following general form: Creator, Year of Data Publication, Title of Dataset, Publisher, Dataset identifier. For example:

Shaver, G. 1989. Above ground biomass in acidic tussock tundra experimental site, 1989, Arctic LTER, Toolik, Alaska. Arctic LTER, Marine Biological Lab, Woods Hole, Ma 02543. 1989gsttbm http://ecosystems.mbl.edu/arc/terrest/biomass/index.shtml 

4 ) Acknowledgement. The Data User should acknowledge any institutional support or specific funding awards referenced in the metadata accompanying this dataset in any publications where the Data Set contributed significantly to its content. Acknowledgements should identify the supporting party, the party that received the support, and any identifying information such as grant numbers. For example:

Data sets were provided by the Arctic LTER. This material is based upon work supported by the National Science Foundation under Grants #DEB-981022, 9211775, 8702328; #OPP-9911278, 9911681, 9732281, 9615411, 9615563, 9615942, 9615949, 9400722, 9415411, 9318529; #BSR 9019055, 8806635, 8507493.

5 ) Notification. The Data User will notify the Data Set Contact when any derivative work or publication based on or derived from the Data Set is distributed. The Data User will provide the data contact with two reprints of any publications resulting from use of the Data Set and will provide copies, or on-line access to, any derived digital products. Notification will include an explanation of how the Data Set was used to produce the derived work.
6 ) Collaboration. The Data Set has been released in the spirit of open scientific collaboration. Data Users are thus strongly encouraged to consider consultation, collaboration and/or co-authorship with the Data Set Creator.

By accepting this Data Set, the Data User agrees to abide by the terms of this agreement. The Data Owner shall have the right to terminate this agreement immediately by written notice upon the Data User's breach of, or non-compliance with, any of its terms. The Data User may be held responsible for any misuse that is caused or encouraged by the Data User's failure to abide by the terms of this agreement.

Disclaimer

While substantial efforts are made to ensure the accuracy of data and documentation contained in this Data Set, complete accuracy of data and metadata cannot be guaranteed. All data and metadata are made available "as is". The Data User holds all parties involved in the production or distribution of the Data Set harmless for damages resulting from its use or interpretation.

Dataset URLs:METADATA: HTML, Rich Text, XML(EML compliant)
DATA: Comma Delimited, Excel file with Metadata and data, Dataset via LTER Data Poral
Dataset ID:19832012KuparukChl.03
Dataset Title:Epilithic and sestonic chlorophyll for the Kuparuk River during the summers of 1983 - 2012
Investigator 1: 
First Name:Breck
Last Name:Bowden
Organization:University of Vermont
Address line 2:303D Aiken Center
Address line 3:Rubenstein School of Environment and Natural Resources
City:Burlington
State:Vermont
Zip Code:05405
Country:USA
Investigator 2: 
First Name:Bruce
Last Name:Peterson
Organization:Marine Biological Laboratory
Address line 2:7 MBL St.
Address line 3:The Ecosystems Center
City:Woods Hole
State:MA
Zip Code:02543
Country:USA
Investigator 3: 
First Name:Linda
Last Name:Deegan
Organization:Marine Biological Laboratory
Address line 2:7 MBL St.
Address line 3:The Ecosystems Center
City:Woods Hole
State:MA
Zip Code:02543
Country:USA
Investigator 4: 
First Name:Alex
Last Name:Huryn
Organization:University of Alabama
Address line 2:
Address line 3:
City:Tuscaloosa
State:AL
Zip Code:
Country:USA
Investigator 5: 
First Name:Elissa
Last Name:Schuett
Organization:University of Vermont
City:Burlington
State:Vermont
Country:USA
Investigator 6: 
First Name:Adrian
Last Name:Green
Organization:
Address line 2:
Address line 3:
City:
State:
Zip Code:
Country:
Investigator 7: 
First Name:Karie
Last Name:Slavik
Associate Investigators:
Keywords:chlorophyll a, epilithic algae, sestonic, Kuparuk River, primary production, streams, arctic streams
Abstract:Sestonic and epilithic chlorophyll for the Kuparuk River during the summers of 1983 - 2012. Water was filtered for sestonic chlorophyll and rocks from designated locations on the Kuparuk River were scrubbed for epilithic chlorophyll approximately three times each summer. Phosphorus addition has occurred since 1983; station sites are relative distance from the original 1983 phosphorus dripper. Stations include sites in a reference, recovery, and fertilized reach. Reaches were defined based on the location of phosphorous addition (see methods). Phosphoric acid is added to the stream to increase the soluble reactive phosphorus by 0.32 uM. Both sestonic and epilithic chlorophyll a were analyzed for the Kuparuk from 1983 to current.
For questions about the Metadata and data contact the Investigators.
For information about this web site contact:
Arctic LTER Information Manager
The Ecosystems Center
Marine Biological Lab
7 MBL St
Woods Hole, MA 02543
Phone (508) 289 7496
Email: arc_im@mbl.edu
Online URL: http://ecosystems.mbl.edu/ARC/
DATA FILE INFORMATION:
Data File URL http://metacat.lternet.edu/das/dataAccessServlet?docid=knb-lter-arc.10304&urlTail=streams/stream_chemistry/data/1983-2012_Kuparuk_Chl.csv
Data File Name 1983-2012_Kuparuk_Chl
Beginning Date 7/22/1983
End Date 8/11/2012
Number of Data Records 4846
Other Files to Reference
Availability Status 1
Quality Control Information field replicates and lab replicates of samples as well as quality assurance of data
Maintenance Description ongoing data collection, updated annually
Log of Changes: 3/2013: EBS organized metadata and data into this file; 4/2013: JPB moved to new spreadsheet and updated Research Locations.
Version 2: Checked keywords against the LTER network preferred list and replaced non-preferred terms. Jim L 15Jan14
Version 3: 8/2013: EBS added 2011 data, added new column for 2011 P-dripper, added Total Chl and Phaeophytin data; 12/2013: JPB added 2012 data. 16Jan2014-JD Checked file, run through new keywords, spelling, uploaded
 
RESEARCH LOCATION:                                
Location Name Kuparuk -0.47k Kuparuk -0.3k Kuparuk -0.177k Kuparuk 0.0k Kuparuk 0.3k Kuparuk 0.56k Kuparuk 0.74k Kuparuk 1.0k Kuparuk 1.39k Kuparuk 1.8k Kuparuk 2.0k Kuparuk 2.5k Kuparuk 3.0k Kuparuk 4.0k Hershey Creek  
Geographic Description Kuparuk River, North Slope of Alaska, reference reach, upstream of the fertilized reach. Kuparuk River, North Slope of Alaska, reference reach, upstream of the fertilized reach. Kuparuk River, North Slope of Alaska, reference reach, upstream of the fertilized reach. Kuparuk River, North Slope of Alaska. Current site of phosphorous addition. Kuparuk River, North Slope of Alaska, re-fertilized reach, formerly the recovery reach after historic fertilization. Kuparuk River, North Slope of Alaska, re-fertilized reach, formerly the recovery reach after historic fertilization. Kuparuk River, North Slope of Alaska, re-fertilized reach, formerly the recovery reach after historic fertilization. Kuparuk River, North Slope of Alaska, re-fertilized reach, formerly the recovery reach after historic fertilization. Kuparuk River, North Slope of Alaska, re-fertilized reach, formerly the recovery reach after historic fertilization. Kuparuk River, North Slope of Alaska, fertilized reach. Kuparuk River, North Slope of Alaska, fertilized reach. Kuparuk River, North Slope of Alaska, fertilized reach. Kuparuk River, North Slope of Alaska, fertilized reach. Kuparuk River, North Slope of Alaska, fertilized reach. Tributary of the Kuparuk River, North Slope of Alaska.  
Location Bounding Box                                
West Bounding Coordinate                                
East Bounding Coordinate                                
North Bounding Coordinate                                
South Bounding Coordinate                                
OR if single point location                                
Latitude 68.6374451277778 68.6383996472222 68.6380604555556 68.6391037472222 68.6407478666667 68.64205425 68.6431598416667 68.6446591138889 68.6463382 68.6478126416667 68.6495472222222 68.6522845416667 68.6536672416667 68.6587855777778 68.6444935871761  
Longitude -149.386685694444 -149.389229505556 -149.391843363889 -149.394325569444 -149.400561111111 -149.403500566667 -149.403267013889 -149.405160461111 -149.410026416667 -149.415654311111 -149.416152463889 -149.41517665 -149.420855111111 -149.424464330556 -149.412131309509  
Elevation 753.32 752.799 750.601 750.566 747.744 746.268 746.552 744.061 741.689 738.873 737.074 734.785 732.173 726.919 751.338  
Link to Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map View on Google Map  
                                 
 
TAXONOMIC COVERAGE:
Organisms studied
 
Methods:Samples were collected at designated stations along the Kuparuk RiverIn 1983, phosphorus addition through dripping began at the site designated as 0.0k. Stations downstream of 0.0k have positive numbers and upstream have negative numbers. The reach upstream of the phosphorus addition has remained the reference reach for the duration of this dataset. Downstream of the original phosphorus addition was the fertilized reach. In 1985, the phosphorus dripper moved to 0.59k, creating a recovery reach from 0.0k to 0.59k. In 1996, the dripper was moved downstream to 1.4k, creating a recovery reach between 0.6k and 1.39k. The historic recovery reach from 0k to 0.59k had not been fertilzed for 11 years, so it was then considered part of the reference reach after 1996. Reach information can be found in data for specific time periods. Sestonic chlorophyll a was sampled by filtering 500 mL stream water through a 47mm filter. The fitlers were returned to the lab and placed in 10ml of 90% buffered acetone and extracted for 24 hours. The samples were then read on a Turner 111 (1990-1991) and Turner 450 (1992-1997), TurnerDesigns 10AU (1998-2009) Fluorometers.
Epilithic chlorophyll was sampled using either a "2x2" or "whole rock" scrubbing method.
2x2 method: Epilithic chlorophyll was sampled three times on rocks from
riffles. Using a plastic 35mm slide mount to partition a known
area, the algae was scraped from the rock surface with a brass
brush. The brushed area of the rock, the brush and slide holder
were then rinsed and the slurry collected and brought up to a
volume of 56 ml. Five rocks were sampled from each riffle
station.
Whole rock method: Whole rock samples were also collected from riffles.
The upper surface of rocks fitting into an area of 546cm^2 ( +/- 18cm) (363 cm2 ( +/- 18cm) in 1996) were scrubbed with a brass brush.
The slurry was brought to a volume of 1 L.The samples were returned to the lab
where they were filtered through a GF/C or GF/F glass fiber filter.
The filters were then placed in 10 ml of 90% buffered acetone and extracted in the dark for 24 hours. The samples
were then read on a Turner 111 (1990-1991) and Turner 450 (1992-1997), TurnerDesigns 10AU (1998-2012) Fluorometers.

Reference Citations: R.G. Wetzel and G.E. Likens. 1979. Limnological Methods. Saunders. Philadelphia.
Notes: In 2004 several adjustments were made to the protocol from 2003. Extraction took place at approximately 4C using chilled acetone. A multipoint curve was analyzed using a chl a stock std. A Turner Designs solid standard could then be utilized as a check. GF/F 25mm filters were used. Additionally, chl a was corrected for pheophytin following EPA 445 and data is reported in corrected chl a. For more information please reference the chlorophyll protocol available on the Arctic LTER website.

Data Table

Variable Name Variable Description Data Type Units DateTime Format Code Information Missing Value Code
Site LTER Site code text        
River site of measurement (named) text        
Date Date datetime   dd-mmm-yy    
Station (km) Distance from P dripper, usually in kilometers. Negative numbers indicate sites upstream from fertilization; positive numbers are downstream. text        
1983 P-dripper location Treatment reach based on the location of the dripper in 1985 (reference or fertilized) text        
1985 P-dripper location Treatment reach based on the location of the dripper in 1985 (reference, recovery, or fertilized) text        
1996 P-dripper location Treatment reach based on the location of the dripper in 1996 (reference, recovery, or fertilized) text        
2011 P-dripper location Treatment reach based on the location of the dripper in 2011 (reference, re-fertilized 1, re-fertilized 2, or fertilized) text        
Type Type of sample (e.g. sestonic, whole rock epilithic, 2x2 epilithic) text       -1111=no data | -9999=below detection limit
Epilithic Total Chlorophyll (ug/cm^2) Total chlorophyll (not corrected for phaeophytin) (ug/cm^2) for epilithic samples number microgramPerCentimeterSquared     -1111=no data | -9999=below detection limit
Epilithic Chl a (ug/cm^2) Phaeophyton corrected Chl a (ug/cm^2) for whole rock scrubs number microgramPerCentimeterSquared      
Epilithic phaeophytin (ug/cm2) Phaeophytin concentration (ug/cm^2) for epilithic samples number microgramPerCentimeterSquared      
Sestonic Total Chlorophyll (ug/L) Total chlorophyll (not corrected for phaeophytin) (ug/L) for sestonic samples number microgramPerLiter      
Sestonic Chl a (ug/L) Phaeophyton corrected Chl a (ug/L) for sestonic number microgramPerLiter      
Sestonic phaeophytin (ug/L) Phaeophytin concentration (ug/L) for sestonic samples number microgramPerLiter      
Comments Notes about the data text        
Please contact arc_im@mbl.edu with questions, comments, or for technical assistance regarding this web site.