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The re-use of scientific data has the potential to greatly increase communication, collaboration and synthesis within and among disciplines, and thus is fostered, supported and encouraged. Permission to use this dataset is granted to the Data User free of charge subject to the following terms:

1) Acceptable use. Use of the dataset will be restricted to academic, research, educational, government, recreational, or other not-for-profit professional purposes. The Data User is permitted to produce and distribute derived works from this dataset provided that they are released under the same license terms as those accompanying this Data Set. Any other uses for the Data Set or its derived products will require explicit permission from the dataset owner.
2 ) Redistribution. The data are provided for use by the Data User. The metadata and this license must accompany all copies made and be available to all users of this Data Set. The Data User will not redistribute the original Data Set beyond this collaboration sphere.
3 ) Citation. It is considered a matter of professional ethics to acknowledge the work of other scientists. Thus, the Data User will properly cite the Data Set in any publications or in the metadata of any derived data products that were produced using the Data Set. Citation should take the following general form: Creator, Year of Data Publication, Title of Dataset, Publisher, Dataset identifier. For example:

Shaver, G. 1989. Above ground biomass in acidic tussock tundra experimental site, 1989, Arctic LTER, Toolik, Alaska. Arctic LTER, Marine Biological Lab, Woods Hole, Ma 02543. 1989gsttbm http://ecosystems.mbl.edu/arc/terrest/biomass/index.shtml 

4 ) Acknowledgement. The Data User should acknowledge any institutional support or specific funding awards referenced in the metadata accompanying this dataset in any publications where the Data Set contributed significantly to its content. Acknowledgements should identify the supporting party, the party that received the support, and any identifying information such as grant numbers. For example:

Data sets were provided by the Arctic LTER. This material is based upon work supported by the National Science Foundation under Grants #DEB-981022, 9211775, 8702328; #OPP-9911278, 9911681, 9732281, 9615411, 9615563, 9615942, 9615949, 9400722, 9415411, 9318529; #BSR 9019055, 8806635, 8507493.

5 ) Notification. The Data User will notify the Data Set Contact when any derivative work or publication based on or derived from the Data Set is distributed. The Data User will provide the data contact with two reprints of any publications resulting from use of the Data Set and will provide copies, or on-line access to, any derived digital products. Notification will include an explanation of how the Data Set was used to produce the derived work.
6 ) Collaboration. The Data Set has been released in the spirit of open scientific collaboration. Data Users are thus strongly encouraged to consider consultation, collaboration and/or co-authorship with the Data Set Creator.

By accepting this Data Set, the Data User agrees to abide by the terms of this agreement. The Data Owner shall have the right to terminate this agreement immediately by written notice upon the Data User's breach of, or non-compliance with, any of its terms. The Data User may be held responsible for any misuse that is caused or encouraged by the Data User's failure to abide by the terms of this agreement.

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While substantial efforts are made to ensure the accuracy of data and documentation contained in this Data Set, complete accuracy of data and metadata cannot be guaranteed. All data and metadata are made available "as is". The Data User holds all parties involved in the production or distribution of the Data Set harmless for damages resulting from its use or interpretation.

Dataset URLs:METADATA: HTML, Rich Text, XML(EML compliant)
DATA: Comma Delimited, Excel file with Metadata and data, Dataset via LTER Data Poral
Dataset ID:2003gsimCFN.03
Dataset Title:Chloroform-extractableN and d15N within 15N addition plots for Aug 2003
Investigator 1: 
First Name:Gus
Last Name:Shaver
Organization:Ecosystems Center
Address line 2:Marine Biological Lab
Address line 3:
City:Woods Hole
State:MA
Zip Code:02543
Country:USA
Investigator 2: 
First Name:Yuriko
Last Name:Yano
Organization:Ecosystems Center
Address line 2:Marine Biological Lab
City:Woods Hole
State:MA
Zip Code:02543
Country:USA
Investigator 3: 
First Name:Bill
Last Name:Holmes
Organization:
Address line 2:
City:
State:
Zip Code:
Country:
Investigator 4: 
First Name:Don
Last Name:Zak
Investigator 5: 
First Name:Anne
Last Name:Giblin
Investigator 6: 
First Name:Ed
Last Name:Rastteter
Investigator 7: 
First Name:Jim
Last Name:Laundre
Associate Investigators:
Keywords:chloroform-extractable nitrogen, microbial biomass, nitrate, ammonium, dissolved organic nitrogen, soil organic nitrogen, nitrogen-15, inorganic nutrients, organic matter
Abstract:Pool size and d15N values for chloroform-extractable N, extractable-N, and non-extractable N pools. Samples collected in Aug. 2003 from 1st Organic Layer of 15N addition plots in Imnavait watershed.
1st Organic Layer = the upper 10 cm of organic soil or, if the organic layer was < 10 cm thick, the entire layer (e.g., there was never > 4 cm of organic soil at Crest).
For questions about the Metadata and data contact the Investigators.
For information about this web site contact:
Arctic LTER Information Manager
The Ecosystems Center
Marine Biological Lab
7 MBL St
Woods Hole, MA 02543
Phone (508) 289 7496
Email: arc_im@mbl.edu
Online URL: http://ecosystems.mbl.edu/ARC/
DATA FILE INFORMATION:
Data File URL http://metacat.lternet.edu/das/dataAccessServlet?docid=knb-lter-arc.10268&urlTail=terrest/bulk/data/2003gsimCFN.csv
Data File Name 2003gsimCFN.dat
Beginning Date 8/20/2003
End Date 8/20/2003
Number of Data Records 96
Other Files to Reference
Availability Status 1
Quality Control Information
Maintenance Description
Log of Changes: Updated Metadata sheet
Version 2: Updated units to current standards. Missing values changed to #N/A. Updated metadata form to newer version (without site sheet). Fixed discrepency with header variable names. CH 24 Jan 2013 Updated to newer metadata with site sheet. CH March 2013.
Version 3: Checked keywords against the LTER network preferred list and replaced non-preferred terms. Jim L 27Jan14
 
RESEARCH LOCATION:                                            
Location Name Imnaviat watershed- eastern slope Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One  
Geographic Description Eastern slope of Imnavait watershed. ~1km south of main weir, ~4 km south of Dalton Hwy. Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description  
Location Bounding Box                                            
West Bounding Coordinate                                            
East Bounding Coordinate                                            
North Bounding Coordinate                                            
South Bounding Coordinate                                            
OR if single point location                                            
Latitude 68.6081 In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees  
Longitude -149.3078 In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees  
Elevation In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters  
Link to Google Map View on Google Map                                          
                                             
 
TAXONOMIC COVERAGE:
Organisms studied
 
Methods:Field:
One core per plot (total 12 cores) were collected in late August, 2003. The cores were immediately shipped to U of Michigan and deep froze (-80-degree C) until extraction.

Lab:
Subsamples of the cores were sequentially extracted with K2SO4 for d15N diffusion of NH4, NO3, and DON. Another set of subsamples used to determine chloroform-labile N using CHCl3 fumigation.
Note: The chroloform-labile N should not be reported as microbial N, because the conventional conversion factor was determined for temperate soils, and it is likely that the factor could be very different for Arctic soils.
----------------------------------------------------------------------------------------------------------------------------------
Chloroform-extractable 15N of Aug 2003 soils (Analyzed by Bill Holmes @ U. of Michigan for Yuriko Yano @ Ecosystems Ctr. MBL)

Subdivide fresh soils:
10 g to dry at 60C for total 15N analysis
12.00+/-0.02 g to extract with K2SO4 (CFE-NF, extractable NH4+ and NO3-, DON-15N)
10 g to fumigate and extract with K2SO4 (CFE-FUM)

Extractable NH4+ and NO3- and DON (CFE-NF)
extract with 0.5N K2SO4
vacuum filter through quartz fiber filter with wide Buchner funnel
microfilter extract
analyze for NH4+ and NO3- on rapid-flow analyzer (RFA)
diffuse NH4+ and NO3-
digest for 15N DON
diffuse NO3-

CFE (FUM)
fumigate soils
extract with K2SO4
vacuum filter through quartz fiber filter with wide Buchner funnel
digest extract (alkaline persulfate digest)
analyze for NH4+ and NO3- on RFA
diffuse NH4+ and NO3-

Total 15N analysis
dry soil at 60C
grind
burn
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Calculation of N pool size and 15N recovery into chloroform-extractable N pool and non-extractable pool:

We found out later that the diffusion method used above would not be able to separate DIN from DON (i.e., DON would be diffused with NH4 and NO3). Becuase "DIN" and "DON" were diffused sequencially, 15N and N pool size of DON and TDN were back calculated. A mixing model was used to claculate 15N recovery to TDN.

Data Table

Variable Name Variable Description Data Type Units DateTime Format Code Information Missing Value Code
Date Date of soil sampling datetime   DD-MMM-YY    
Pool N pool names (DON= dissolved organic N; MB-N= chroloform-labile N; SON= soil organic N) text        
Plot Soil sampling sites within 15N addition plots text        
Location CR=crest; LS NWT= lower slope non-watertrack; LS WT=lower slope water; FS WT=foot slope watertrack; FS NWT=foot slope non-watertrack; R=riparian wet sedge text        
d15N del-15N values (o/oo) number partPerThousand     #N/A=Missing or Not Measured
SoilN bulk N concentration of soil samples (microgramPerMilligram) number microgram     #N/A=Missing or Not Measured
Npool_per_g Size of N-pools per gram bulk soil (microgramPerMilligram) number microgram     #N/A=Missing or Not Measured
APE Atom % excess of 15N number percent     #N/A=Missing or Not Measured
15Nrecov_m2 15N recovered per area (m2) (micromolePerMeterSquared) number micromole     #N/A=Missing or Not Measured
15Nrecov_pc Percent 15N recovered number percent      

Please contact arc_im@mbl.edu with questions, comments, or for technical assistance regarding this web site.