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Conditions of Use

The re-use of scientific data has the potential to greatly increase communication, collaboration and synthesis within and among disciplines, and thus is fostered, supported and encouraged. Permission to use this dataset is granted to the Data User free of charge subject to the following terms:

1) Acceptable use. Use of the dataset will be restricted to academic, research, educational, government, recreational, or other not-for-profit professional purposes. The Data User is permitted to produce and distribute derived works from this dataset provided that they are released under the same license terms as those accompanying this Data Set. Any other uses for the Data Set or its derived products will require explicit permission from the dataset owner.
2 ) Redistribution. The data are provided for use by the Data User. The metadata and this license must accompany all copies made and be available to all users of this Data Set. The Data User will not redistribute the original Data Set beyond this collaboration sphere.
3 ) Citation. It is considered a matter of professional ethics to acknowledge the work of other scientists. Thus, the Data User will properly cite the Data Set in any publications or in the metadata of any derived data products that were produced using the Data Set. Citation should take the following general form: Creator, Year of Data Publication, Title of Dataset, Publisher, Dataset identifier. For example:

Shaver, G. 1989. Above ground biomass in acidic tussock tundra experimental site, 1989, Arctic LTER, Toolik, Alaska. Arctic LTER, Marine Biological Lab, Woods Hole, Ma 02543. 1989gsttbm http://ecosystems.mbl.edu/arc/terrest/biomass/index.shtml 

4 ) Acknowledgement. The Data User should acknowledge any institutional support or specific funding awards referenced in the metadata accompanying this dataset in any publications where the Data Set contributed significantly to its content. Acknowledgements should identify the supporting party, the party that received the support, and any identifying information such as grant numbers. For example:

Data sets were provided by the Arctic LTER. This material is based upon work supported by the National Science Foundation under Grants #DEB-981022, 9211775, 8702328; #OPP-9911278, 9911681, 9732281, 9615411, 9615563, 9615942, 9615949, 9400722, 9415411, 9318529; #BSR 9019055, 8806635, 8507493.

5 ) Notification. The Data User will notify the Data Set Contact when any derivative work or publication based on or derived from the Data Set is distributed. The Data User will provide the data contact with two reprints of any publications resulting from use of the Data Set and will provide copies, or on-line access to, any derived digital products. Notification will include an explanation of how the Data Set was used to produce the derived work.
6 ) Collaboration. The Data Set has been released in the spirit of open scientific collaboration. Data Users are thus strongly encouraged to consider consultation, collaboration and/or co-authorship with the Data Set Creator.

By accepting this Data Set, the Data User agrees to abide by the terms of this agreement. The Data Owner shall have the right to terminate this agreement immediately by written notice upon the Data User's breach of, or non-compliance with, any of its terms. The Data User may be held responsible for any misuse that is caused or encouraged by the Data User's failure to abide by the terms of this agreement.

Disclaimer

While substantial efforts are made to ensure the accuracy of data and documentation contained in this Data Set, complete accuracy of data and metadata cannot be guaranteed. All data and metadata are made available "as is". The Data User holds all parties involved in the production or distribution of the Data Set harmless for damages resulting from its use or interpretation.

Dataset URLs:METADATA: HTML, Rich Text, XML(EML compliant)
DATA: Comma Delimited, Excel file with Metadata and data
Dataset ID:2012_MAT_06_NP_FWEB_JCM.01
Dataset Title:Belowground foodweb biomass and soil CN and bulk density from moist acidic tundra nutrient addition plots (since 2006) sampled August 2012.
Investigator 1: 
First Name:John
Last Name:Moore
Organization:Natural Resource Ecology Laboratory
Address line 2:200 West Lake Street
Address line 3:
City:Fort Collins
State:CO
Zip Code:80523-1499
Country:USA
Investigator 2: 
First Name:Rodney
Last Name:Simpson
Organization:Natural Resource Ecology Laboratory
Address line 2:200 West Lake Street
City:Fort Collins
State:CO
Zip Code:80523-1499
Country:USA
Investigator 3: 
First Name:Akihiro
Last Name:Koyama
Organization:Natural Resource Ecology Laboratory
Address line 2:200 West Lake Street
City:Fort Collins
State:CO
Zip Code:80523-1499
Country:USA
Investigator 4: 
First Name:Greg
Last Name:Selby
Organization:Natural Resource Ecology Laboratory
Address line 2:200 West Lake Street
City:Fort Collins
State:CO
Zip Code:80523-1499
Country:USA
Associate Investigators:
Keywords:moist acidic tundra, protozoa, nematodes, rotifers, tardigrades, enchytraeids
Abstract:Biomass of soil rotifers, tardigrades, enchytraeids, protozoa and nematode groups from organic and mineral soils in moist acidic tundra nutrient addition plots (since 2006) sampled August 2012.
For questions about the Metadata and data contact the Investigators.
For information about this web site contact:
Arctic LTER Information Manager
The Ecosystems Center
Marine Biological Lab
7 MBL St
Woods Hole, MA 02543
Phone (508) 289 7496
Email: arc_im@mbl.edu
Online URL: http://ecosystems.mbl.edu/ARC/
DATA FILE INFORMATION:
Data File URL http://metacat.lternet.edu/das/dataAccessServlet?docid=knb-lter-arc.10487&urlTail=terrest/microbes/data/2012_MAT_06_NP_FWEB_JCM.csv
Data File Name 2012_MAT_06_NP_FWEB_JCM
Beginning Date 8/1/2012
End Date 8/31/2012
Number of Data Records 180
Other Files to Reference
Availability Status Type 1
Quality Control Information
Maintenance Description
Log of Changes: Data and metadata entered and updated R.Simpson Dec.2013, uploaded JD13Dec13
 
RESEARCH LOCATION:      
Location Name LTER Low Nutrient Moist Acidic Tussock Tundra Select Site or enter New One  
Geographic Description LTER Low Nutrient Moist Acidic Tussock Tundra Enter Description  
Location Bounding Box      
West Bounding Coordinate      
East Bounding Coordinate      
North Bounding Coordinate      
South Bounding Coordinate      
OR if single point location      
Latitude 68.622865 In Decimal Degrees  
Longitude -149.608545 In Decimal Degrees  
Elevation 758 In Meters  
Link to Google Map View on Google Map    
       
 
TAXONOMIC COVERAGE:
Organisms studied
 
Methods:In August 2012, soil samples were collected from control, and N+P plots from within a set of treatments in Moist Acidic Tundra plots established in 2006. At the time of sample collection we separated the soil into organic horizon, organic/mineral interface, and the upper 5cm of the mineral soil, and measured the depth of each layer. We determined percent moisture for all soils and quantified protozoa and nematode the biomass. Protozoa densities were estimated from 10 g soil samples using Most Probable Number MPN (Darbyshire et al. 1974; Ingham 1994). 10 g soil was serially diluted 10-1-10-6. Using a 24-well tissue culture plate, four replicate wells of each of the six dilutions were created by adding 0.5 ml soil solution to the wells (Rusterholz and Mallory 1994). E. coli, a bacterium food source for protozoa was added to each of the wells. Protozoa were separated into the broad categories of Amoebae, Flagellates and Ciliates. Nematodes were isolated from 20 g of soil samples using Baermann Funnels (Hall 1996). Nematodes were preserved in formalin, counted and identified to functional group based on the morphology of the stoma and stylet (Niles 1994). Rotifers, Tardigrades, and Enchytraeids were isolated from 5 g of soil using the methods of Peters et al. (1993).

References
Darbyshire, J. F., R. F. Wheatley, M. P. Greaves, and R. H. E. Inkson. 1974. A rapid micro method for estimating bacteria and protozoa in soils. Rev. Ecol. Biol. Sol. 11:465-475.
Hall, G.S. 1996. Methods for the examination of organismal diversity in soils and sediments. CAB International: New York.
Ingham, E.R. 1994. Protozoa. In: R.W. Weaver et al. (ed.), Methods of Soil Analysis. Part 2: Microbiological and Biochemical properties. Soil Science Society of America, Inc., USA. pp. 491-512.
Niles, R. 1994. Identification of nematode feeding groups. Unpublished manuscript.
Peters, U., Koste, W., and Westheide W. 1993. A quantitative method to extract moss-dwelling rotifers. Hydrobiologia 255-256: 330-341.

Data Table

Variable Name Variable Description Data Type Units DateTime Format Code Information Missing Value Code
Block Arctic LTER blocks text        
N+P Treatment Control vs +NP: 1989 +NP = Fertilization treatment since 1989 | 2006 Low+NP = Low fertilization treatment since 2006 | 2006 High+NP = High fertilization treatment since 2006 text        
Depth Soil Layer text        
Quadrat Subsample within plot text        
Flagellates mg flagellates/g dry soil number milligramPerGram     -9999 = missing
Amoebae mg amoebae/g dry soil number milligramPerGram     -9999 = missing
Ciliates mg ciliates/g dry soil number milligramPerGram     -9999 = missing
Bacterivorous Nematodes mg bacterivorous nematodes/g dry soil number milligramPerGram     -9999 = missing
Fungivorous Nematodes mg fungivorous nematodes/g dry soil number milligramPerGram     -9999 = missing
Free Phytophagous Nematodes mg free phytophagous nematodes/g dry soil number milligramPerGram     -9999 = missing
Root-Associated Phytophagous Nematodes mg root-associated phytophagous nematodes/g dry soil number milligramPerGram     -9999 = missing
Total Phytophagous Nematodes mg total phytophagous nematodes/g dry soil number milligramPerGram     -9999 = missing
Omnivorous Nematodes mg omnivorous nematodes/g dry soil number milligramPerGram     -9999 = missing
Predatory Nematodes mg predatory nematodes/g dry soil number milligramPerGram     -9999 = missing
Total Rotifers mg rotifers/g dry soil number milligramPerGram     -9999 = missing
Tardigrades mg tardigrades/g dry soil number milligramPerGram     -9999 = missing
Enchytraeids mg enchytraeids/g dry soil number milligramPerGram     -9999 = missing
Please contact arc_im@mbl.edu with questions, comments, or for technical assistance regarding this web site.