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The re-use of scientific data has the potential to greatly increase communication, collaboration and synthesis within and among disciplines, and thus is fostered, supported and encouraged. Permission to use this dataset is granted to the Data User free of charge subject to the following terms:

1) Acceptable use. Use of the dataset will be restricted to academic, research, educational, government, recreational, or other not-for-profit professional purposes. The Data User is permitted to produce and distribute derived works from this dataset provided that they are released under the same license terms as those accompanying this Data Set. Any other uses for the Data Set or its derived products will require explicit permission from the dataset owner.
2 ) Redistribution. The data are provided for use by the Data User. The metadata and this license must accompany all copies made and be available to all users of this Data Set. The Data User will not redistribute the original Data Set beyond this collaboration sphere.
3 ) Citation. It is considered a matter of professional ethics to acknowledge the work of other scientists. Thus, the Data User will properly cite the Data Set in any publications or in the metadata of any derived data products that were produced using the Data Set. Citation should take the following general form: Creator, Year of Data Publication, Title of Dataset, Publisher, Dataset identifier. For example:

Shaver, G. 1989. Above ground biomass in acidic tussock tundra experimental site, 1989, Arctic LTER, Toolik, Alaska. Arctic LTER, Marine Biological Lab, Woods Hole, Ma 02543. 1989gsttbm http://ecosystems.mbl.edu/arc/terrest/biomass/index.shtml 

4 ) Acknowledgement. The Data User should acknowledge any institutional support or specific funding awards referenced in the metadata accompanying this dataset in any publications where the Data Set contributed significantly to its content. Acknowledgements should identify the supporting party, the party that received the support, and any identifying information such as grant numbers. For example:

Data sets were provided by the Arctic LTER. This material is based upon work supported by the National Science Foundation under Grants #DEB-981022, 9211775, 8702328; #OPP-9911278, 9911681, 9732281, 9615411, 9615563, 9615942, 9615949, 9400722, 9415411, 9318529; #BSR 9019055, 8806635, 8507493.

5 ) Notification. The Data User will notify the Data Set Contact when any derivative work or publication based on or derived from the Data Set is distributed. The Data User will provide the data contact with two reprints of any publications resulting from use of the Data Set and will provide copies, or on-line access to, any derived digital products. Notification will include an explanation of how the Data Set was used to produce the derived work.
6 ) Collaboration. The Data Set has been released in the spirit of open scientific collaboration. Data Users are thus strongly encouraged to consider consultation, collaboration and/or co-authorship with the Data Set Creator.

By accepting this Data Set, the Data User agrees to abide by the terms of this agreement. The Data Owner shall have the right to terminate this agreement immediately by written notice upon the Data User's breach of, or non-compliance with, any of its terms. The Data User may be held responsible for any misuse that is caused or encouraged by the Data User's failure to abide by the terms of this agreement.

Disclaimer

While substantial efforts are made to ensure the accuracy of data and documentation contained in this Data Set, complete accuracy of data and metadata cannot be guaranteed. All data and metadata are made available "as is". The Data User holds all parties involved in the production or distribution of the Data Set harmless for damages resulting from its use or interpretation.

Dataset URLs:METADATA: HTML, Rich Text, XML(EML compliant)
DATA: Comma Delimited, Excel file with Metadata and data, Dataset via LTER Data Poral
Dataset ID:AlaskaDaltonTransect1990_2007.04
Dataset Title:Plant litter, soil, plants and fungal fruiting bodies 15N, 13C, percent C and N along Dalton Highway, Alaska 2004, 1990, 2007.
Investigator 1: 
First Name:John (J.C.)
Last Name:Weber
Organization:Ecosystems Center
Address line 2:Marine Biological Laboratory
Address line 3:
City:Woods Hole
State:MA
Zip Code:02543
Country:US
Investigator 2: 
First Name:John
Last Name:Hobbie
Organization:Ecosystems Center
Address line 2:Marine Biological Laboratory
City:Woods Hole
State:MA
Zip Code:02543
Country:
Associate Investigators:
Keywords:Alaska, Dalton highway, leaf litter, plant foliage, fungal fruiting bodies, mushrooms, nitrogen-15, 13C, soil
Abstract:The data set includes 15N and 13C for plant litter, soil, plants and fungal fruiting bodies (mycorrhizae), percent C (soil organic matter and percent N from samples collected in three separate trips (1990, 2004, 2007) along the transect of the Dalton Highway (AK) extending from the Yukon River on the south to Prudhoe Bay on the north.
For questions about the Metadata and data contact the Investigators.
For information about this web site contact:
Arctic LTER Information Manager
The Ecosystems Center
Marine Biological Lab
7 MBL St
Woods Hole, MA 02543
Phone (508) 289 7496
Email: arc_im@mbl.edu
Online URL: http://ecosystems.mbl.edu/ARC/
DATA FILE INFORMATION:
Data File URL http://metacat.lternet.edu/das/dataAccessServlet?docid=knb-lter-arc.10106&urlTail=terrest/microbes/data/AlaskaDaltonTransect1990_2007.csv
Data File Name AlaskaDaltonTransect1990_2007.xls
Beginning Date 8/1/1990
End Date 8/1/2007
Number of Data Records 170
Other Files to Reference
Availability Status
Quality Control Information
Maintenance Description
Log of Changes: Version1: 3Jan2011 Submitted data. Jiml
Version2: 21Sep11 Updated metadata form to new one and corrected path to xlm file in the harvestfile
Version 3: Missing values changed to #N/A. CH 24 Jan 2013 Updated to newer metadata with site sheet. CH March 2013.
Version 4: Corrected URL. It had xlsfiles in the path. Jim L. 19Jun14
 
RESEARCH LOCATION:                                            
Location Name Dalton Highway Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One  
Geographic Description Dalton Highway, AK, Yukon River to Prudhoe Bay Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description  
Location Bounding Box                                            
West Bounding Coordinate -149.724                                          
East Bounding Coordinate -148.463                                          
North Bounding Coordinate 70.222                                          
South Bounding Coordinate 65.884                                          
OR if single point location                                            
Latitude In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees  
Longitude In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees  
Elevation In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters  
Link to Google Map                                            
                                             
 
TAXONOMIC COVERAGE:
Organisms studied Alder, Betula, Picea mariana, Picea glauca, Calamagrostis, Carex, Cassiope, Empetrum, Ledum, Moss, Rubus, Vaccinium, White Spruce, Salix, Bolete, Cortinarius, Purple Cortinarius, Lactarius, Leccinium, Melanoleuca, Russula,
 
Methods: For the 1990 collections the locations, methods of chemical analysis, and the stable isotope analyses are described in Schulze, E. D., F. S. Chapin, and G. Gebauer. 1994. (Nitrogen nutrition and isotope differences among life forms at the northern treeline of Alaska. Oecologia 100:406-412.) For the 2004 and 2007 collections, the methods of chemical analysis are described in Hobbie et al. (2009).
Hobbie, J. E., and E. A. Hobbie. 2006. N-15 in symbiotic fungi and plants estimates nitrogen and carbon flux rates in Arctic tundra. Ecology 87:816-822.
Hobbie, J. E., E. A. Hobbie, H. Drossman, M. Conte, J. C. Weber, J. Shamhart, and M. Weinrobe. 2009. Mycorrhizal fungi supply nitrogen to host plants in Arctic tundra and boreal forests: N-15 is the key signal. Canadian Journal of Microbiology 55:84-94.
Soil samples in 2004 were collected by removing the intact whole leaves and collecting the decomposing top layer (litter) and the underlying 0-5 cm organic fraction. In 2007, a 10-20 cm deep, 6 cm diameter core was taken at a representative site next to known ectomycorrhizal plants. After collection, soil samples were immediately placed on ice and frozen within six hours. In the laboratory, each core from the 2007 transect was thawed and sectioned into three approximately equal depth organic layers and two approximately equal depth mineral layers for analysis of ergosterol, soil moisture, and soil organic matter. 15N analyses. The1990 collection was analyzed as described in Schulze et al. (1994). The Denali 1998 collection was analyzed on a Finnigan Delta-Plus linked to a Carlo Erba NC2500 elemental analyzer (Finnigan MAT GmbH, Bremen, Germany) located at the US Environmental Protection Agency in Corvallis, Oregon. Laboratory standards for isotopic measurements were pine needles (NIST 1575) and acetanilide. The precision of duplicate samples was 0.2. The 2004 collection was analyzed at the UC-Davis Stable Isotope Facility with the same methods described in Hobbie and Hobbie (2006). Laboratory standards included peach leaves (NIST 1547) and apple leaves (NIST 1515). The 2007 collections were analyzed at the University of New Hampshire on a Finnigan Delta-Plus isotope ratio mass spectrometer coupled to a Costech CHN analyzer. Precision on duplicate samples was 0.2. Concurrently run standards included acetanilide, pine needles (NIST 1575a), and apple leaves (NIST 1515). To express variations in the 15N:14N ratio for samples in a tractable form, they are referenced against a universal standard and calculated as d15N values, defined as d15Nsample = [(15N/14N)sample /(15N/14N)standard 1] x 1000, with the standard the 15N:14N value of atmospheric N2, which, therefore, has a d15N value of 0. Ergosterol. This fungal-specific sterol, a component of the cell membrane, estimates living fungal biomass in soils. See references in Hobbie et al. (2009). We modified the saponification methods described by Bth (2001) and de Ridder-Duine et al. (2006) for HPLC quantification of ergosterol. In the laboratory, thawed samples were sectioned by horizon (e.g., organic and mineral), each layer sub-sectioned by depth, and each subsection sieved (2 mm screen). Duplicate or triplicate sub-samples (0.5 g) of the sieved soil fractions were saponified by heating for 90 min at 70?C in 2 ml of methanol and 0.5 ml of 2 M NaOH after brief vortex mixing. After cooling, 1 ml methanol and 3 ml pentane were added and samples vortex-mixed for 20 s. Samples were centrifuged briefly to eliminate emulsions and the upper pentane phase, containing ergosterol, was collected. The pentane addition, extraction and centrifugation was repeated twice more, but with 2 ml pentane per extraction step. The pentane (upper) phase was collected and combined with previous extracts. The combined pentane extracts were dried under nitrogen flow, dissolved in 1.0 ml methanol, filtered through a 0.45 m pore size PTFE syringe filter (Millipore, Corporation, Billerica, Massachusetts, USA) and injected into a Waters Acquity UPLC system with a binary solvent manager, a sample manager, and a photodiode array detector (PDA) (Waters Corporation, Milford, Massachusetts, USA). Separation was on an Acquity UPLC BEH C18 1.7 micron pore size, 2.1 x 50 mm reverse phase column maintained at 40C. A 5 l injection of each extract was eluted with 92% methanol-8% water at a flow rate of 0.5 ml min-1. Using Millenium software (Waters) the UV spectrum of the ergosterol peak at ~2 min was compared with standard ergosterol (Fluka Company, St. Louis, Missouri, USA) and was quantified at 282 nm with calibration standards in the range of 0.05 20 ppm. Calibration provided linear fit (r2 > 0.999) with standards in all runs with a limit of detection (S/N 3:1) of ~ 30 ppb. Spike recoveries determined by the method of de Ridder-Duine et al. (2006) are 824%, in agreement with prior literature values (Bth 2001). Variance for duplicate or triplicate samples resulting from sample inhomogeneity, extraction, and instrumental sources were generally less than 5% and are calculated as standard deviation for each sample. Soil moisture was determined gravimetrically on a separate sub-sample by drying to constant weight. Soil organic matter was calculated from the loss in mass of the dry soil after heating at 500?C for 12 hours.


Data Table

Variable Name Variable Description Data Type Units DateTime Format Code Information Missing Value Code
Sample Type of sample collected text        
Site Number Number applies only to 2004 samples. For 1990 it is Dalton Highway miles text        
Site Name Name of site text        
Date Date of collection. For Schulze et al. (1994) it is August 1990 -- no other dates are given in paper. datetime   dd-mmm-yyyy    
C (ug)_MEAN Mean C content of litter, soil, or vegetation number microgramPerGram     #N/A=Missing or Not Measured
C (ug)_STD DEV Standard deviation of C content number microgramPerGram     #N/A=Missing or Not Measured
% C_MEAN Mean Percent C of sample number percent     #N/A=Missing or Not Measured
% C_STD DEV Percent C of sample standard deviation number percent     #N/A=Missing or Not Measured
d13C_MEAN Mean delta 13C of sample number partPerThousand     #N/A=Missing or Not Measured
d13C_STD DEV Standard deviation of delta 13C of sample number partPerThousand     #N/A=Missing or Not Measured
N (ug)_MEAN Mean N content of litter, soil, or vegetation number microgramPerGram     #N/A=Missing or Not Measured
N (ug)_STD DEV Standard deviation of N content number microgramPerGram     #N/A=Missing or Not Measured
% N_MEAN Mean Percent N of sample number percent     #N/A=Missing or Not Measured
% N_STD DEV Percent N of sample standard deviation number percent     #N/A=Missing or Not Measured
d15N_MEAN Mean delta 15N of sample number partPerThousand     #N/A=Missing or Not Measured
d15N_STD DEV Standard deviation of delta 15N of sample number partPerThousand     #N/A=Missing or Not Measured
Lat. (N) Sample location latitude text       #N/A=Missing or Not Measured
Lat. (N) decimal degree Sample location latitude in decimal degrees number degree     #N/A=Missing or Not Measured
Long. (W) Sample location longitude in degrees and minutes text       #N/A=Missing or Not Measured
Elev. (ft) Elevation of sampling location number foot     #N/A=Missing or Not Measured
Depth (cm) Depth of thaw at site number centimeter     #N/A=Missing or Not Measured
Comment Comments on data text       #N/A=Missing or Not Measured

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