Program Version: 1.2 (30-Oct-2018) Program (Re)Started: 10/30/2018 19:24:04, time = 0.80838 d Sample loop purge time for Air (min): 2.00; On port: 10 Sample loop purge time for MC1 (min): 2.00; On port: 1 Sample loop purge time for MC2 (min): 2.00; On port: 2 Loop time (hr): 1.00 --------------------------- Experiment Description ---------------------------- Sample collected from Siders Pond and brought back to lab at 18:30 Sample filter through 300 um nytex mesh Sample collected at deep station at 2.5 m ------------------------------------------------------------------------------- Note Number: 1 (10/30/2018 20:04:18 time = 0.83632 d) Dilution at 0.1 1/d begun now, at about 20:00 30-Oct-2018 ------------------------------------------------------------------------------- Note Number: 2 (10/31/2018 12:04:41 time = 1.50325 d) Collected samples for cell counts and carbon substrates (11:45) ------------------------------------------------------------------------------- Note Number: 3 (11/02/2018 15:04:56 time = 3.62843 d) Sampled all parameters on 11-2-18 at 14:25 and euk cell counts at 15:05 ------------------------------------------------------------------------------- Note Number: 4 (11/02/2018 15:29:54 time = 3.64576 d) Changed gas feed rate to MC1 and MC2 from 20 to 10 sccm (mL/min) ------------------------------------------------------------------------------- Note Number: 5 (11/02/2018 17:30:24 time = 3.72944 d) New gas standards were used to calibrate the oxigraf O2/CO2 analyzer ------------------------------------------------------------------------------- Note Number: 6 (11/02/2018 17:32:24 time = 3.73083 d) New standards were used to calibration oxigraf analyzer, and these produced slight different values for the composition of the current air tank, however, the new values are extremely close to the old ones an within the error of the oxigraf instrument ------------------------------------------------------------------------------- Note Number: 7 (11/02/2018 17:34:02 time = 3.73197 d) ------------------------------------------------------------------------------- Note Number: 8 (11/05/2018 19:42:13 time = 6.82098 d) Collected samples at 19:40 ------------------------------------------------------------------------------- Note Number: 9 (11/14/2018 12:11:37 time = 15.50807 d) Sample from both chemostats taken. This is the last sample at the 0.01 1/d dilution rate ------------------------------------------------------------------------------- Note Number: 10 (11/14/2018 12:34:18 time = 15.52382 d) Flow rate was increased to 2.06 mL/min on the media feed and to 0.021 mL/min on the carbon source feed at 12:33. ------------------------------------------------------------------------------- Note Number: 11 (11/15/2018 12:30:40 time = 16.52130 d) Increased pH in medium in barrel from around 6.5 to 7.57 with NaOH. It was also noted that more algae was growing in barrel than expected, so increasing the ligh blocking around the barrel ------------------------------------------------------------------------------- Note Number: 12 (11/16/2018 15:58:59 time = 17.66596 d) It was noticed that the carbon medium pump was actually pumping at around 0.25 mL/min instead of 0.021 mL/min due to some kind of software bug in the pump. It is unknown if the pump rate was correct during the 0.1 1/d dilution rate, but the guess is that it was. For now, we are leaving the carbon pump at 0.25 mL/min, but the pump is working correctly now ------------------------------------------------------------------------------- Note Number: 13 (11/19/2018 18:28:10 time = 20.76956 d) Changed dilution rate from 1 to 10 1/d. Medium set to 27.3 L/d and carbon source changed to 2.727 L/d at 18:27 -------------------------------------------------------------------------------