Program Version: 1.2 (30-Oct-2018) Program (Re)Started: 02/05/2019 17:47:29, time = 0.74131 d Sample loop purge time for Air (min): 2.00; On port: 10 Sample loop purge time for MC1 (min): 2.00; On port: 1 Sample loop purge time for MC2 (min): 2.00; On port: 2 Loop time (hr): 1.00 --------------------------- Experiment Description ---------------------------- Starting Exp2. A carboy of water from the surface of siders pond was collected from shore near the town center at approximately 14:30, which was passed through 335 um nytex filter. Chemostats were filled with pond water at approximately 16:30. Dilution rate was set to zero. ------------------------------------------------------------------------------- Program Version: 1.2 (30-Oct-2018) Program (Re)Started: 02/05/2019 18:58:34, time = 0.79067 d Sample loop purge time for Air (min): 2.00; On port: 10 Sample loop purge time for MC1 (min): 2.00; On port: 1 Sample loop purge time for MC2 (min): 2.00; On port: 2 Loop time (hr): 1.00 --------------------------- Experiment Description ---------------------------- Restarting after fixing problem with sample loop mass flow meter ------------------------------------------------------------------------------- Note Number: 1 (02/05/2019 19:00:17 time = 0.79186 d) ------------------------------------------------------------------------------- Note Number: 2 (02/05/2019 19:00:49 time = 0.79223 d) first measurement ------------------------------------------------------------------------------- Note Number: 3 (02/06/2019 10:57:56 time = 1.45690 d) Changing carbon pump and recalibrating at 10:58 ------------------------------------------------------------------------------- Note Number: 4 (02/06/2019 13:02:58 time = 1.54373 d) Re-starting calibration at 13:03 ------------------------------------------------------------------------------- Note Number: 5 (02/06/2019 14:25:11 time = 1.60082 d) Finished calibrating pumps at 14:25 ------------------------------------------------------------------------------- Note Number: 6 (02/06/2019 14:39:18 time = 1.61063 d) Turned nutrients and carbon on at 14:39; while doing so, noticed that the MC1 was looking turbulent. Realized that DO probe was not fully immersed so the propeller was hitting the probe. This may explain the odd DO offset? Will monitor for another 1-2 hours to ensure still gas tight/dripping looks good (ABM) ------------------------------------------------------------------------------- Note Number: 7 (02/06/2019 15:32:05 time = 1.64728 d) Emptied out waste containers at 15:32 and changed so each has its own container (to track approximate flow rate) ------------------------------------------------------------------------------- Note Number: 8 (02/06/2019 15:59:14 time = 1.66613 d) Dilution rate was turned on at 14:39 (t = 1.61 d). Flow rate of nutrients set to 2.06 mL/min and carbon medium at 0.02 mL/min ------------------------------------------------------------------------------- Note Number: 9 (02/06/2019 16:57:14 time = 1.70641 d) Testing gas tightness of sample loop during sampling of MCs ------------------------------------------------------------------------------- Note Number: 10 (02/07/2019 18:08:04 time = 2.75560 d) pH of nutrient medium measured at 6.85, so increased pH to 7.1 with NaOH ------------------------------------------------------------------------------- Note Number: 11 (02/07/2019 18:10:05 time = 2.75700 d) Noticed that gas sample flow loop meter started reading high values around 16:00; however, the flow meter in the oxygraf O2/CO2 meter still shows flows around 230 mL/min, so it looks like MFM is giving bad readings again but not sure why ------------------------------------------------------------------------------- Note Number: 12 (02/07/2019 18:35:40 time = 2.77477 d) Disconnected MFM on sample loop and removed water. Also changed orientation of MFM to minimize water collecting in it. However, I do not know why this is happening, but we will need to keep an eye on it. It may be necessary to replace the Napion dryier ------------------------------------------------------------------------------- Note Number: 13 (02/07/2019 19:13:22 time = 2.80095 d) just purging system a bit ------------------------------------------------------------------------------- Program Version: 1.3 (7-Feb-2019) Program (Re)Started: 02/07/2019 19:57:50, time = 2.83183 d Sample loop purge time for Air (min): 2.00; On port: 10 Sample loop purge time for MC1 (min): 2.00; On port: 1 Sample loop purge time for MC2 (min): 2.00; On port: 2 Loop time (hr): 1.00 --------------------------- Experiment Description ---------------------------- Restaring with new version 1.3 that allows 4 digits of MFM flow readings ------------------------------------------------------------------------------- Program Version: 1.3 (8-Feb-2019) Program (Re)Started: 02/08/2019 16:00:19, time = 3.66689 d Sample loop purge time for Air (min): 2.00; On port: 10 Sample loop purge time for MC1 (min): 2.00; On port: 1 Sample loop purge time for MC2 (min): 2.00; On port: 2 Loop time (hr): 1.00 --------------------------- Experiment Description ---------------------------- Restarting after fixing MFM output for readAnalyzersAndStore routine ------------------------------------------------------------------------------- Note Number: 14 (02/11/2019 09:43:06 time = 6.40493 d) (ABM) at 9:20 noticed there was lots of biofilm growth on MC1 DO probe. Paused sampling, took out probe and capped chemostat, then wiped off probe. Also cleaned biofilm from the walls of the chemostat. Will push back sampling to this afternoon. ------------------------------------------------------------------------------- Note Number: 15 (02/11/2019 14:39:11 time = 6.61054 d) At 14:39 started sampling MC1 ------------------------------------------------------------------------------- Note Number: 16 (02/11/2019 14:59:26 time = 6.62461 d) At 14:59 started sampling for MC2 ------------------------------------------------------------------------------- Note Number: 17 (02/11/2019 15:09:03 time = 6.63128 d) Ended first sampling timepoint at 15:09 ------------------------------------------------------------------------------- Note Number: 18 (02/13/2019 09:57:51 time = 8.41517 d) increased N2 gas flow to nafion dryer from 300 mL/min to 500 mL/min however, I notice that the MFM for the sample loop is back to reporting values that are correct, ~ 200 mL/min ------------------------------------------------------------------------------- Note Number: 19 (02/13/2019 14:19:12 time = 8.59667 d) Collected sample of filter (orange) to look at under scope and added foil to tubing leading into grwoth chamber at 14:19 on 2/13/19 ------------------------------------------------------------------------------- Note Number: 20 (02/13/2019 18:30:00 time = 8.77083 d) Started replacing nutrient tubing at 18:09; ended at 18:29 on 2/13/19 Hypothesis is that there is photosynthetic growth in tubing between media drum and filter ------------------------------------------------------------------------------- Note Number: 21 (02/14/2019 18:09:37 time = 9.75668 d) New nafion dryer installed, so analyzers were recalibrated and air tank which is the same as at the start, was remeasured. Slightly different, but well within the error of the analyzer ------------------------------------------------------------------------------- Note Number: 22 (02/15/2019 13:11:06 time = 10.54937 d) Suzanne noticed medium connection to the top of MC1 had popped off this morning (15 Feb 2019), so she reconnected, but by ~12:00 it had popped off again. Poking rod through SS MC fitting pushed out a "cigarette butt" like wad of biomass pushed into MC1. It is currently floating around. ------------------------------------------------------------------------------- Note Number: 23 (02/15/2019 13:20:46 time = 10.55609 d) Installed a 250W infra red heater focused on Valco valve and mini pump to prevent condensation ------------------------------------------------------------------------------- Note Number: 24 (02/15/2019 16:27:03 time = 10.68545 d) On 2/15 16:27 Starting to change feed tubing to keep C and nutrients separate and replacing the entire sampling feed tubing setup on MC1 ------------------------------------------------------------------------------- Note Number: 25 (02/15/2019 16:56:53 time = 10.70617 d) Finished changing input tubing at 16:47 on 2/15; switched pumps back on ------------------------------------------------------------------------------- Note Number: 26 (02/15/2019 17:57:00 time = 10.74792 d) Changed over to SS 1/4" tubing from SS drum to inside the growth chamber to minimize light exposure to tubing as well is minimize carbon input to minimal nutrient media before it enters chemostats ------------------------------------------------------------------------------- Note Number: 27 (02/15/2019 18:02:46 time = 10.75192 d) Waste containers from chemostats emptied ------------------------------------------------------------------------------- Note Number: 28 (02/15/2019 18:24:13 time = 10.76682 d) Exchanged 1 L of media from one chemostat to the other to insure microbial communities are similar between the two MCs. Headspace was contaiminated with air, so gas dynamics will take a day or so to restabilize ------------------------------------------------------------------------------- Note Number: 29 (02/19/2019 11:55:59 time = 14.49721 d) Biofilm on DO probe in MC1 cleaned around 10:00 19-Feb-2019 ------------------------------------------------------------------------------- Note Number: 30 (02/20/2019 12:41:02 time = 15.52850 d) On 2/20/19: Sampled MC1 at 10:38 and MC2 at 10:59. Sampled feed at 11:19 ------------------------------------------------------------------------------- Note Number: 31 (02/22/2019 11:05:29 time = 17.46214 d) On 2/22 at 11:05 wiped pH probe in MC2 ------------------------------------------------------------------------------- Note Number: 32 (02/22/2019 13:57:12 time = 17.58139 d) 2/22/19 at 13:57 Start sampling ------------------------------------------------------------------------------- Note Number: 33 (02/25/2019 18:32:27 time = 20.77254 d) 2/25/19 at 18:30 swtiched to new nutrient media with higher conc N&P -------------------------------------------------------------------------------