Program Version: 1.3 (8-Feb-2019) Program (Re)Started: 03/06/2019 13:47:39, time = 0.57476 d Sample loop purge time for Air (min): 2.00; On port: 10 Sample loop purge time for MC1 (min): 2.00; On port: 1 Sample loop purge time for MC2 (min): 2.00; On port: 2 Loop time (hr): 1.00 --------------------------- Experiment Description ---------------------------- Inoculated with Siders Pond water at 11:50 on 6Mar2019 ------------------------------------------------------------------------------- Note Number: 1 (03/06/2019 14:28:31 time = 0.60314 d) ------------------------------------------------------------------------------- Note Number: 2 (03/06/2019 14:29:02 time = 0.60350 d) Finished calibrations at 1429, restarting sampling loop ------------------------------------------------------------------------------- Note Number: 3 (03/06/2019 17:04:08 time = 0.71120 d) Started dripping nutrient and carbon media at 17:00 at 0.1 d-1 ------------------------------------------------------------------------------- Note Number: 4 (03/06/2019 17:05:05 time = 0.71186 d) ------------------------------------------------------------------------------- Note Number: 5 (03/07/2019 11:57:26 time = 1.49822 d) This was actually changed at 10:30 7-Feb-2019 (t = 1.438 d) ------------------------------------------------------------------------------- Note Number: 6 (03/08/2019 13:31:37 time = 2.56362 d) 13:06 Added paddles to nutrient media and attached 0.2 um filters ------------------------------------------------------------------------------- Note Number: 7 (03/13/2019 18:13:23 time = 7.75929 d) MCs sampled around 15:00 today (this caused the spike in DO and O2 ------------------------------------------------------------------------------- Note Number: 8 (03/14/2019 15:34:37 time = 8.64904 d) ------------------------------------------------------------------------------- Note Number: 9 (03/14/2019 17:47:39 time = 8.74142 d) Switching to new nutrient media feed setup ------------------------------------------------------------------------------- Note Number: 10 (03/14/2019 18:50:01 time = 8.78473 d) Stopped old media at 1748; started new media at 1830 ------------------------------------------------------------------------------- Note Number: 11 (03/15/2019 15:08:49 time = 9.63112 d) At around 11:00 15-Mar-2019 a samples were taken from the nutrient feed medium and MC 1 for phosphate measurement using the Hanna checker. The results are: Medium feed (1/4 dilution): 9.41 uM (final concentration) Medium feed (1/2 dilution): 8.39 uM MC 1 (no dilution): 0.65 uM Medium feed should be around 21 uM, so more than 1/2 was likely removed as FePO4 during filtration ------------------------------------------------------------------------------- Note Number: 12 (03/15/2019 15:32:14 time = 9.64738 d) At 15:21 and 15:23 on 15-Mar-2019 we added 10 mL of 3 mM phosphate for MC1 and 2, respectively. Pulled existing nutrient media out and filtered through a 0.2 um acrodisk. Also added in sterivex inline. ------------------------------------------------------------------------------- Note Number: 13 (03/15/2019 18:45:47 time = 9.78179 d) On 15-Mar2019 at 18:42 added in another 10 mL 3 mM phosphate (no response to prior addition) ------------------------------------------------------------------------------- Note Number: 14 (03/16/2019 13:19:00 time = 10.55486 d) On 16-Mar2019 at 13:18 turned C pump back on (power outage last night) and set to "autostart" ------------------------------------------------------------------------------- Note Number: 15 (03/19/2019 15:37:50 time = 13.65127 d) On 19-Mar2019 at 10:58 collected feed. 13:57 calibrated carbon pump. 15:15 and 15:24 collected sample from MC1 and MC2, respectively ------------------------------------------------------------------------------- Note Number: 16 (03/19/2019 16:08:08 time = 13.67231 d) On 19-Mar2019 Switched to new media, removed sterivex inline (16:04) ------------------------------------------------------------------------------- Note Number: 17 (03/19/2019 16:16:07 time = 13.67786 d) At 16:16 on 19-Mar2019 found C pump spinning higher than it was set at (probably 10x as fast); started and stopped again, now it is fine. This has happened before, especially after a calibration. ------------------------------------------------------------------------------- Note Number: 18 (03/22/2019 15:37:19 time = 16.65092 d) New N2 tank on-line ------------------------------------------------------------------------------- Note Number: 19 (03/24/2019 19:45:34 time = 18.82331 d) 24_Mar 2019 at 19:45 - switching to new nurtient media and sampling feed ------------------------------------------------------------------------------- Note Number: 20 (03/24/2019 22:00:19 time = 18.91689 d) On 24-Mar2019 sampled feed at 20:05; realized carbon flow rate low for MC1 so moved tubing on pumphead and did a calibration. Checked flow rate at 21:08 and it is much better and equal between chemostats (0.201 and 0.196 for MC1 and MC2, respectively, for 10 minute check. ------------------------------------------------------------------------------- Note Number: 21 (03/25/2019 09:43:50 time = 19.40544 d) Sampled chemostats at 9:11 on 25-Mar2019. Stopped C pump to switch to C13 at 9:31. C13 carbon turned on at 9:40! ------------------------------------------------------------------------------- Note Number: 22 (03/25/2019 12:28:18 time = 19.51965 d) ------------------------------------------------------------------------------- Note Number: 23 (03/25/2019 14:42:09 time = 19.61260 d) For 3hour timepoint, sampled gas at 12:35 and chemostats at 12:45 on 25-Mar2019 ------------------------------------------------------------------------------- Note Number: 24 (03/25/2019 15:30:22 time = 19.64609 d) ------------------------------------------------------------------------------- Note Number: 25 (03/25/2019 16:15:09 time = 19.67719 d) Sampled 6hr timepoint at 15:45 and 15:46 on 25-Mar2019 ------------------------------------------------------------------------------- Note Number: 26 (03/25/2019 21:18:26 time = 19.88780 d) ------------------------------------------------------------------------------- Note Number: 27 (03/25/2019 22:17:21 time = 19.92871 d) Sampled 12 hr timepoint at 21:40 on 25-Mar2019 ------------------------------------------------------------------------------- Note Number: 28 (03/26/2019 10:03:37 time = 20.41918 d) Sampled 24 hour timepoint at 9:40 on 26-Mar2019 ------------------------------------------------------------------------------- Note Number: 29 (03/26/2019 21:17:51 time = 20.88740 d) ------------------------------------------------------------------------------- Note Number: 30 (03/26/2019 22:01:59 time = 20.91804 d) Sampled 36 hour timepoint at 21:40 on 26-Mar2019 ------------------------------------------------------------------------------- Note Number: 31 (03/27/2019 10:05:45 time = 21.42066 d) Sampled 48 hour timepoint at 9:40 on 27-Mar2019 ------------------------------------------------------------------------------- Note Number: 32 (03/28/2019 09:16:32 time = 22.38648 d) ------------------------------------------------------------------------------- Note Number: 33 (03/28/2019 10:04:30 time = 22.41979 d) Sampled 72 hour timepoint at 9:40 on 28-Mar2019 ------------------------------------------------------------------------------- Note Number: 34 (03/29/2019 08:27:17 time = 23.35228 d) Checked flow rate and altered pumps: to 0.022 on carbon, 1.82 to nutrients at 8:27 on 29-Mar2019 ------------------------------------------------------------------------------- Note Number: 35 (03/29/2019 10:01:59 time = 23.41804 d) Sampled 96 hour timepoint at 9:40 on 29-Mar2019 ------------------------------------------------------------------------------- Note Number: 36 (03/30/2019 10:06:10 time = 24.42095 d) Samploed 120 hour timepoint at 9:40. Sampled feed 10:02 on 30-Mar2019 ------------------------------------------------------------------------------- Note Number: 37 (03/30/2019 11:12:28 time = 24.46699 d) Replaced nutrient media at 11:10 and increased flow rate to 2.01 mL/min ------------------------------------------------------------------------------- Note Number: 38 (04/04/2019 09:24:45 time = 29.39219 d) ------------------------------------------------------------------------------- Note Number: 39 (04/04/2019 10:36:21 time = 29.44191 d) Sampled at 9:40 on 4-April2019. Sampled feed at 10:02 ------------------------------------------------------------------------------- Note Number: 40 (04/11/2019 08:26:51 time = 36.35198 d) Turned off pumps on 6-April 2019 at 8:35. Kept monitoring system on. -------------------------------------------------------------------------------