Plum Island Ecosystems LTER Database

Acceptance and utilization of LTER data requires that:

(1) The Principal Investigator be sent a notice stating reasons for acquiring any data and a description of the publication intentions.
(2) The Principal Investigator of the data set be sent a copy of the report or manuscript prior to submission and be adequately cited in any resultant publications.
(3) A copy of any resultant publications should be sent to:

Principal Investigator
Ecosystems Center
Marine Biological Laboratory
7 MBL St.
Woods Hole, MA 02543

Dataset URLs:METADATA: HTML, Rich Text, XML(EML compliant)
DATA: Comma Delimited, Excel file with Metadata and data
Dataset ID:HTL-PR-Isotope.02
Dataset Title:Stable isotope (carbon, nitrogen and sulfur) data for primary producers and consumer organisms in the Plum Island Sound Estuary.
Investigator 1: 
First Name:Linda
Last Name:Deegan
Address line 1:Ecosystems Center
Address line 2:MBL
Address line 3:7 MBL St
City:Woods Hole
State:MA
Zip Code:02543
Country:USA
Associate Investigators:Charles Hopkinson, Hap Garritt
Keywords:LTER, PIE, Plum Island Ecosystems, Massachusetts, stable isotope, nitrogen, carbon, sulfur, food web, trophic, fish, invertebrates, organic, particulate, marsh, vegetation
Abstract:Flora and fauna stable isotope study to help characterize organic matter/primary production sources important to the food web of the Plum Island Sound estuary. Sampling occured during 1993 and 1994.
Contact: Plum Island Ecosystems LTER Information Manager
The Ecosystems Center
Marine Biological Lab
7 MBL St
Woods Hole, MA 02543
Phone (508) 289 7485
Email: pie_im@mbl.edu
Online URL: http://pie-lter.ecosystems.mbl.edu
DATA FILE INFORMATION:
Data File URL http://ecosystems.mbl.edu/PIE/data/HTL/data/HTL-PR-Isotope.csv
Data File Name HTL-PR-Isotope
Beginning Date 6/15/1993
End Date 6/16/1994
Number of Data Records 82
Other Files to Reference
Availability Status Type 1
Quality Control Information
Maintenance Description
Log of Changes: Version 02: February 20, 2014, data and metadata updated to comply with importation to Drupal and LTER PASTA. Used MarcrosExportEML_HTML (working)pie_excel2007_Sep2013.xlsm 9/30/13 02:57 PM for QA/QC to EML 2.1.0
 
RESEARCH LOCATION: EST-PR-P24 EST-PR-P20.2 EST-PR-P11.6 EST-PR-P22 EST-RO-R9.6 EST-SO-S5.6-Rowley HTL-PR-P12.8 HTL-PR-P16.1 HTL-PR-P19 HTL-PR-P20.8 HTL-PR-P22.9 HTL-PR-P23.4 HTL-PR-P24.1 HTL-RO-R9.5 HTL-SO-S2 HTL-SO-S-3.1 HTL-SO-S2.5 HTL-SO-S2.5 HTL-SO-S6 HTL-SO-S8.2 HTL-SO-S9.2  
Geographic Description Plum Island estuarine water column transect station, Parker River pond below Central St Dam in Byfield, MA, 24 km from Plum Island Sound mouth reference Plum Island estuarine water column transect station, Parker River, 20.2 km from Plum Island Sound mouth reference Plum Island estuarine water column transect station, Parker River, 11.6 km from Plum Island Sound mouth reference Plum Island estuarine water column transect station, Parker River, 22 km from Plum Island Sound mouth reference Rowley River water column station off dock at MBL Rowley Field Station, Rowley MA Plum Island estuarine water column transect station, Plum Island Sound, near Rowley River mouth, 5.6 km from Plum Island Sound mouth reference Parker River, Newbury, MA, trawl station, located between the Little and Mill Rivers, several hundred meters east of the railroad bridge. Parker River, Newbury, MA, trawl station, located just east of the first meander in the river east of U.S. Route 1, approximately 0.8 km east of this road. Parker River, Newbury, MA, trawl station, located several hundred meters west of Thurlow's Bridge (Middle Road). Parker River, Newbury, MA, trawl station, located about 2 km west of Thurlow's Bridge (Middle Road). Parker River, Newbury, MA, trawl station, located about 1 km east of the falls at Orchard and Central Street in Byfield. Parker River, ~ 0.5 km downstream of ponded area below dam Parker River, pond area below Central St dam Rowley River flat, Rowley, MA shore line beach seine station located on a mud flat that bordered the salt marsh on the south side of the Rowley River about 0.75 km downstream from the PIE LTER Rowley Field Station, Essex County Greenbelt property 95 Railroad Avenue. Great Neck, Ipswich, MA, shoreline beach seine station located several hundred meters north of DMF's 1967 Little Neck station near Pavilion Beach, a narrow strip of land that separates Great Neck from Little Neck on the western shore of Plum Island Sound. Castle Neck, Ipswich, MA, trawl station, located at the mouth of Plum Island Sound. Great Neck, Ipswich, MA, trawl station, near the mouth of Plum Island Sound. Bluff's, Ipswich, MA shoreline beach seine station located on the eastern side of Plum Island Sound several hundred meters south of the private residence on Stage Island. Knobs, Rowley, MA, shoreline beach seine station located on sound or West side of Plum Island at the end of the dirt road that extends west past the southern border of the Bill Forward pool on the Parker River National Wildlife Refuge (PRNWR). Nelson's Island, Rowley, MA, shoreline beach seine station located on the PRNWR at the edge of a tidal flat at the end of the dirt road that runs from the refuge parking lot at the end of Stackyard Road across the salt marsh to Nelson's Island. Subheadquarters, Newbury, MA, shoreline beach seine station located on the extensive mud flat just west of the maintenance buildings of the PRNWR.  
Location Bounding Box                                            
West Bounding Coordinate                                            
East Bounding Coordinate                                            
North Bounding Coordinate                                            
South Bounding Coordinate                                            
OR if single point location                                            
Latitude 42.75085508 42.75484502 42.76241138 42.74829383 42.72517443 42.72578704 42.76081412 42.76353793 42.75692394 42.75262433 42.75263608 42.75236466 42.74972247 42.72584126 42.70161801 42.70544185 42.70516169 42.70634998 42.73139029 42.74691643 42.75594527  
Longitude -70.92796269 -70.91250557 -70.84765737 -70.91495895 -70.85490298 -70.81191035 -70.86197714 -70.8862495 -70.90359836 -70.9125256 -70.91949172 -70.92408462 -70.92909587 -70.85434584 -70.79174444 -70.74185558 -70.79048883 -70.78615651 -70.79906604 -70.8210343 -70.81184907  
Elevation                                            
                                             
                                             
 
TAXONOMIC COVERAGE:
Taxonomic Protocols
Organisms studied
 
Methods:Fauna was collected either by hand, hand nets, plankton nets, seines or trawls. Flora was collected either by hand cutting leaves of marsh plants, hand collection of algae , plankton tows or water filtration for particulate organic matter.

Stable Isotope Food Web Monitoring Sample Collection and Processing
Sediments
Collect 5 surface (2cm) cores using 60 ml syringe, place cores in a labeled (date, station, sample type) 4 oz Qorpack jar and in a cooler for transport back to the lab. Freeze if the sample cannot be dried soon.
Dry sediments @ 50o C. After the sample is dried, use a dissecting microscope and check sediment for carbonates using a few drops of 10% HCl on a small subsample, if bubbling occurs then a larger subsample should be acidified and redried for isotope analysis. If carbonates exist it is necessary to remove them, as they will interfere with the 13C analysis. Grind a subsample of the sediments (Wig-L-Bug) for stable isotope analysis and place in a clean scint vial.

Benthic diatoms
Collect benthic diatoms by laying down several (5-7) separate pieces of 210u Nitex on the sediment surface at low tide. Pick a sediment surface that has a golden sheen and still looks fairly wet. If the surface is wet enough the diatoms will migrate up through the Nitex and there will be a fairly pure sample on one side of the Nitex. Use a 500 ml squirt bottle filled with ambient water from the site and gently squirt drops onto the Nitex to get it to lay on the sediment surface without air gaps. After 20 minutes or so, peel the Nitex from the sediment surface and check for an adequate golden sheen on the Nitex, be careful not to contaminate the diatom side with the under side mud or sand. Fold the Nitex (diatom side) carefully in on itself and place in a labeled (date, station, sample type) ziplock bag and put bag in a cooler for transport back to the lab.
At the lab unfold the Nitex with the diatom side on the outside, fold it so that the middle of the Nitex forms a corner facing down and carefully using the ambient water squirt bottle, rinse the diatoms off onto an ashed 25 mm GFF filter that has been set up in a 25mm Gelman filter tower and flask (need replicate filters). When the filter/liquid looks green suction it dry and place the filters into separate labeled (date, station, sample type) small petri dishes. Put petri dishes in a freezer if the samples cannot be dried soon. Dry the filters @ 50o C. After the sample is dried, use a dissecting microscope and check the filters for carbonates using a drop of 10% HCl on a portion of the filter, if bubbling occurs then the whole filter should be acidified and re-dried for isotope analysis. If carbonates exist it is necessary to remove them, as they will interfere with the 13C analysis.

Nereis
Collect Nereis worms by digging with a shovel in the tidal flat sediments; often the peat chunks have more worms. Collect 15 to 20 worms of 2-5 cm lengths and place in a jar with ambient water. Place the jar in a cooler for transport back to the lab.
At the lab transfer the worms to another jar and put clean ambient water in the jar, let the worms sit overnight to purge their guts, then count and record the lengths of worms, towel blot and place worms in a labeled (date, station, sample type) glass scint vial and freeze. Dry the worms @ 50o C and then grind the worms (Wig-L-Bug) for stable isotope analysis and place back in the labeled (date, station, sample type) scint vial.

Mummichog
Using a seine collect 15 to 20 mummichogs, 35-50 mm lengths and place in a labeled (date, station, sample type) ziplock bag and place in cooler for transport back to the lab. At the lab, count and record the lengths of the mummichogs, fillet and save tissue in a labeled (date, station, sample type) glass scint vial and freeze. Dry the mummichogs @ 50o C and then grind the mummichogs (Wig-L-Bug) for stable isotope analysis and place back in the labeled (date, station, sample type) scint vial.

Ribbed mussel
Collect 15 to 20 ribbed mussels from below the Spartina alterniflora channel bank edge and place in a labeled (date, station, sample type) ziplock bag and place in cooler for transport back to the lab.
At the lab, count and record the lengths of the mussels, cut out the abductor muscle and rinse/dip in DI water. Save muscle tissue in a labeled (date, station, sample type) glass scint vial and freeze. Be careful not to get shell fragments mixed in with the abductor muscle. Dry the tissue @ 50o C and then grind the tissue (Wig-L-Bug) for stable isotope analysis and place back in the labeled (date, station, sample type) scint vial.

Particulate Organic Matter (POM)
Collect between 1 liter and 4 liters (depends on station) of mid ebb channel water into labeled (date, station, sample type) bottles recording date and time of day, place in cooler for transport back to the lab.
At the lab, shake the bottles thoroughly and filter water onto ashed, 25 mm GFF filters until clogged (need replicates) and place filters into separate labeled (date, station, sample type) small petri dishes. Put petri dishes in a freezer if the samples cannot be dried soon. Dry the tissue @ 50o C.

Blue mussel
Collect 15 to 20 blue mussels from the front edge of the channel and place in a labeled (date, station, sample type) ziplock bag and place in cooler for transport back to the lab.
At the lab, count and record the lengths of the mussels, cut out the abductor muscle and rinse/dip in DI water. Save muscle tissue in a labeled (date, station, sample type) glass scint vial and freeze. Be careful not to get shell fragments mixed in with the abductor muscle. Dry the tissue @ 50o C and then grind the tissue (Wig-L-Bug) for stable isotope analysis and place back in the labeled (date, station, sample type) scint vial.

Zooplankton (pelagic copepods)
Collect zooplankton from mid channel at mid ebb tide using 150u plankton net. Pour cod end sample into a labeled (date, station, sample type) wide mouth jar, recording date and time of day, place in cooler for transport back the lab. Don’t let jar overheat, the zooplankton must be kept alive.
At the lab, we will conduct a zooplankton light migration technique procedure to purify the sample. Place the jar on a counter and let it settle. When you see a lot of zooplankton in the water column, pour off the water and zooplankton into two glass 250 ml graduated cylinders that have been wrapped with tin foil within 3” of the top. The water height should be about 2 cm above the height of tin foil. Aim a dissecting scope light or flash light at the water surface and let the zooplankton migrate to the light. Set up a vacuum flask for 25 mm filter rig. When there is a dense mass of clean zooplankton at the surface, while avoiding detritus, pipette the zooplankton onto ashed, 25 mm GFF filters (need replicates), squirt filter surface occasionally to migrate zooplankton to the middle of the filter and place filters into separate labeled (date, station, sample type) small petri dishes. Put the petri dishes in a freezer if the samples cannot be dried soon. Dry the filters @ 50o C.

Silversides
Using a seine collect 45 to 60 silversides, 30 – 70 mm lengths and place in a labeled (date, station, sample type) ziplock bag and place in cooler for transport back to the lab. At the lab separate the silversides into 3 subsamples, then count and record the lengths of the silversides for each subsample, then fillet and save the tissue in three separately labeled (date, station, sample type) glass scint vials and freeze. Dry the silversides @ 50o C and then grind them (Wig-L-Bug) for stable isotope analysis and place back in the labeled (date, station, sample type) scint vial.

Mya (softshell clam)
Collect 15 to 20 soft shell clams by digging in the tidal flats and place in a labeled (date, station, sample type) ziplock bag and place in cooler for transport back to the lab. At the lab, count and record the lengths of the clams, cut out the abductor muscle, and rinse/dip in DI water. Save muscle tissue in a labeled (date, station, sample type) glass scint vial and freeze. Be careful not to get shell fragments mixed in with the abductor muscle. Dry the tissue @ 50o C and then grind the tissue (Wig-L-Bug) for stable isotope analysis and place back in the labeled (date, station, sample type) scint vial.

Data Table

Variable Name Variable Description Units Measurement Scale Code Information Number Type DateTime Format Missing Value Code Missing Value Code Explanation
STATION station sampled   nominal       NA NA = no data
Research Location research location name   nominal       NA NA = no data
RiverKm river kilometer going up the estuary where the mouth of the sound is referende or 0 Km   nominal       NA NA = no data
STATE X x coordinates (meters) in MA state plane NAD 83 meter interval   real   NA NA = no data
STATE Y y coordinates (meters) in MA state plane NAD 83 meter interval   real   NA NA = no data
LAT Latitude in NAD 83 geographic coordinates (decimal degrees) degree interval   real   NA NA = no data
LON Longitude in NAD 83 geographic coordinates (decimal degrees) degree interval   real   NA NA = no data
SAL salinity (ppt) partPerThousand interval   real   NA NA = no data
SITE portion of estuary (upper, mid, lower, sound, offshore)   nominal       NA NA = no data
NAME common name of organism sampled   nominal       NA NA = no data
SCI NAME scientific (Latin) name of organism sampled   nominal       NA NA = no data
15N d15N of sample (per mil), isotope ratio 15N/14N partPerThousand ratio   real   NA NA = no data
13C d13C of sample (per mil), isotope ratio 13C/12C partPerThousand ratio   real   NA NA = no data
34S d34S of sample (per mil), isotope ratio 34S/32S partPerThousand ratio   real   NA NA = no data