uid=PIE,o=lter,dc=ecoinformatics,dc=org all public read STP-PR-Mesocosm.01 Plum Island Ecosystems LTER Program Chuck Hopkinson

Georgia Seagrant College Program University of Georgia 229 Marine Sciences Athens GA 30602 USA

Plum Island Ecosystems LTER Program Joe Vallino

Ecosystems Center MBL 7 MBLSt Woods Hole MA 02543 USA

Plum Island Ecosystems LTER Program Linda Deegan

Ecosystems Center MBL 7 MBLSt Woods Hole MA 02543 USA

Plum Island Ecosystems LTER Program Anne Giblin

Ecosystems Center MBL 7 MBLSt Woods Hole MA 02543 USA

Plum Island Ecosystems LTER Program

The Ecosystems Center Marine Biological Lab 7 MBL St Woods Hole MA 02543 USA

(508) 289 7485 pie_im@mbl.edu http://ecosystems.mbl.edu/PIE/ John Hobbie additional investigator Ishi Buffam additional investigator Robert Garritt additional investigator Jane Tucker additional investigator Meredith Hullar additional investigator Mark Benfield additional investigator Ee Lin-Lim Ee LinLim additional investigator 2000 Mesocosm experiment in which the effects of dissolved organic matter and nutrient additions on estuarine food webs were investigated. PIE LTER primary production organic matter nutrients disturbance population dynamics mesocosm trophic food web light ammonium nitrate phosphate organic carbon nitrogen respiration production community fish isotope bacteria chlorophyll microbe Acceptance and utilization of PIE-LTER data requires that: The Principal Investigator be sent a notice stating reasons for acquiring any data and a description of the publication intentions. The Principal Investigator of the data set be sent a copy of the report or manuscript prior to submission and be adequately cited in any resultant publications A copy of any resultant publications should be sent to: Principal Investigator Ecosystems Center Marine Biological Laboratory Woods Hole, MA 02543 http://ecosystems.mbl.edu/PIE/data/STP/STP-PR-Mesocosm.html PIE LTER research site including watersheds and estuary, Essex County, MA -71.22 -70.75 42.83 42.5 1994-09-07 1994-09-29 Experiment finished Version 01: February 9, 2012, updated data and metadata. Used MarcrosExportEML_HTML (working)pie_excel2007.xlsm 2/9/12 1:33 PM for QA/QC to EML 2.1.0 Data Manager

The Ecosystems Center Marine Biological Lab 7 MBL St Woods Hole MA 02543 USA

(508) 289 7485 pie_im@mbl.edu http://ecosystems.mbl.edu/PIE/ Plum Island Ecosystems LTER

The Ecosystems Center Marine Biological Lab 7 MBL St Woods Hole MA 02543 USA

(508) 289 7485 pie_im@mbl.edu http://ecosystems.mbl.edu/PIE/ In the Woods Hole Harbor, MA, four large transparent bags (7m3) were submerged and filled with sea-water. Twelve larval Atlantic silversides (Menidia menidia) were added to each bag. Large fish and other predators were excluded from the mesocosms. The treatments consisted of a control, an organic matter addition, a daily nutrient addition, and organic matter plus nutrient addition. Organic matter additions were 35 mM dissolved organic carbon (GF/F filtered), from leached leaf litter, and was added once at the outset of the experiment. 5 mM NO3-, 0.5 mM PO43-, and 7 uM SiO3 were added daily (at 17:00) in the nutrient addition bags. All mesocosms also received NaH13CO3 to raise d13C of DIC pool to 120 ‰. pers-1 Anne Giblin

The Ecosystems Center Marine Biological Lab 7 MBL St Woods Hole MA 02543 USA

(508) 289 7488 agiblin@mbl.edu Lead PI Hap Garritt

The Ecosystems Center Marine Biological Lab 7 MBL St Woods Hole MA 02543 USA

(508) 289 7485 pie_im@mbl.edu Information Manager The Plum Island Ecosystems (PIE) LTER is an integrated research, education and outreach program whose goal is to develop a predictive understanding of the long-term response of watershed and estuarine ecosystems at the land-sea interface to changes in climate, land use and sea level. The principal study site is the Plum Island Sound estuary, its coupled Parker, Rowley and Ipswich River watersheds and the adjacent coastal ocean, the Gulf of Maine. The PIE LTER focuses on how several aspects of global change influence organic matter and inorganic nutrient biogeochemistry and estuarine foodwebs. The inputs of organic matter and nutrients from land, ocean and marshes interact with the external drivers (climate, land use, river discharge, sea level) to dictate the extent and degree of nutrient and organic matter processing and determine the spatial patterns of estuarine productivity and trophic structure. This material is based upon work supported by the National Science Foundation under Grants OCE-9726921, OCE-0423565. Any opinions, findings, conclusions, or recommendations expressed in the material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation. The Plum Island Ecosystems (PIE) LTER site, is located in northeastern Massachusetts, 42.72 N, 70.85 W. The PIE LTER lies at the interface of a thinly soiled, formerly glaciated New England land mass and the highly productive Gulf of Maine. Three watersheds comprise the estuarine drainage basin: Parker (155 km2), Rowley (26 km2) and Ipswich (404 km2). The Ipswich River watershed is highly urbanized with Boston "bedroom" communities encroaching in the headwater region while the Parker is less urbanized and retains a higher proportion of forest. -71.2198 -70.7568 42.8276 42.4965 0 100 meter STP-PR-Mesocosm.csv Mesocosm experiment in which the effects of dissolved organic matter and nutrient additions on estuarine food webs were investigated. STP-PR-Mesocosm.csv 1 \r\n column , http://ecosystems.mbl.edu/PIE/data/STP/data/STP-PR-Mesocosm.csv DAY DAY Decimal day from the beginning of the experiment number real TREATMENT TREATMENT treatment (CONTROL = control; DOM = dissolved organic matter addition; NUTS = nutrient addition; DOM+NUTS = dissolved organic matter and nutrient addition) CONTROL control DOM dissolved organic matter addition NUTS nutrient addition DOM+NUTS dissolved organic matter and nutrient addition CATEGORY CATEGORY Category of data (WATER CHEMISTRY = NH4, NO3, PO4, Chlorophyll, DOC, POC, PON; PRODUCTION = bacterial production, primary production, size fractionated production; METABOLISM = gross production, respiration, net community production; LIGHT EXTINCTION = light extinction; FISH = fish weight and isotope data, ISOTOPE = isotope data for various dissolved species, particulate size fractions and fish; ZOOPLANKTON = zooplankton species counts; DIATOM = diatom species counts; µFLAGELLATES = µflagellate counts; CILIATES = ciliate species counts, DINOFLAGELLATES = dinoflagellate species counts; BACTERIA = bacterial counts; PAR = light data Category of data (WATER CHEMISTRY = NH4, NO3, PO4, Chlorophyll, DOC, POC, PON; PRODUCTION = bacterial production, primary production, size fractionated production; METABOLISM = gross production, respiration, net community production; LIGHT EXTINCTION = light extinction; FISH = fish weight and isotope data, ISOTOPE = isotope data for various dissolved species, particulate size fractions and fish; ZOOPLANKTON = zooplankton species counts; DIATOM = diatom species counts; µFLAGELLATES = µflagellate counts; CILIATES = ciliate species counts, DINOFLAGELLATES = dinoflagellate species counts; BACTERIA = bacterial counts; PAR = light data TYPE TYPE further description of data type further description of data type SIZE MIN SIZE MIN Size fractionation, minimum size of sample (µm) number real SIZE MAX SIZE MAX Size fractionation, maximum size of sample (µm) number real ORG NUMBER ORG NUMBER number of organisms per Liter of sample numberPerLiter real NH4 NH4 ammonium (µM, NH4-N) micromolePerLiter real NH4 SE NH4 SE standard error of ammonium analysis (µM, NH4-N) micromolePerLiter real PO4 PO4 soluble reactive phosphorus (µM, PO4-P) micromolePerLiter real NO3 NO3 nitrate + nitrite (µM, NO3andNO2-N) micromolePerLiter real DOC DOC dissolved organic carbon (µM as C) micromolePerLiter real POC POC particulate organic carbon (µM as C) micromolePerLiter real PON PON particulate organic nitrogen (µM as N) micromolePerLiter real CHLA CHLA chlorophyll a (µg L-1) microgramPerLiter real Prim Prod Prim Prod primary production (µmol C L-1 d-1) micromolePerLiterPerDay real Gross Prim Prod Gross Prim Prod gross primary production (µmol C mesocosm-1 d-1) dimensionless real RESP RESP respiration (µmol C mesocosm-1 d-1) dimensionless real NCP NCP net community production (µmol C mesocosm-1 d-1) dimensionless real BAC PROD BAC PROD bacterial production (µmol C d-1) micromolePerDay real LTEXT LTEXT light extinction coefficient (m-1) meter real FISH WT FISH WT fish weight (g fish-1) gram real 15N 15N d15N of sample (per mil) partPerThousand real 13C 13C d13C of sample (per mil) partPerThousand real PAR PAR photosynethetically active radiation (µE m-2 s-1) microEinsteinPerMeterSquaredPerSecond real µM = µmoles per liter of solution micrograms per liter micromoles per liter per day micromoles per day ratio of two quantities as parts per thousand (1:1000) micromoles (1E-06 moles of photons) per square meter per second (radiant flux density)