uid=PIE,o=lter,dc=ecoinformatics,dc=org all public read WAT-VA-SigmaNutrients.05 Plum Island Ecosystems LTER Program Wilfred Wollheim

Morse Hall UNH Durham NH 03824 USA

Plum Island Ecosystems LTER Program

The Ecosystems Center Marine Biological Lab 7 MBL St Woods Hole MA 02543 USA

(508) 289 7485 pie_im@mbl.edu http://ecosystems.mbl.edu/PIE/ Corey Lawrence additional investigator Jeff Evans additional investigator Brian Pellerin additional investigator Hap Garritt additional investigator Emily Gaines additional investigator Joe Thouin additional investigator William Lee additional investigator Aaron Strong additional investigator Christina Maki additional investigator Nat Morse additional investigator Amanda Hope additional investigator Rebecca Prosser additional investigator Catherine Caruso additional investigator 2004 Total organic nitrogen, total organic phosphorus, and nitrate concentrations collected frequently by Sigma autosampler (or volunteers in winter) from 5 sites. Sites include three headwater sites of contrasting land use (CC= Forest, SB = suburban, CS = wetland) and at the mouth of the Ipswich and Parker Rivers where they flow into the estuary. PIE LTER inorganic nutrients dissolved nitrate nitrogen nutrients phosphate phosphorus organic carbon Acceptance and utilization of PIE-LTER data requires that: The Principal Investigator be sent a notice stating reasons for acquiring any data and a description of the publication intentions. The Principal Investigator of the data set be sent a copy of the report or manuscript prior to submission and be adequately cited in any resultant publications A copy of any resultant publications should be sent to: Principal Investigator Ecosystems Center Marine Biological Laboratory Woods Hole, MA 02543 http://ecosystems.mbl.edu/PIE/data/WAT/WAT-VA-SigmaNutrients.html Watershed water collection and stream characterization site for Cart Creek (forest landuse), Newbury, MA -70.9165 -70.9165 42.7692 42.7692 Watershed water collection and stream characterization site for Saw Mill Brook (urban landuse), Burlington, MA -71.185 -71.185 42.5236 42.5236 Watershed water collection and stream characterization site for Cedar Swamp (wetland landuse), Reading, MA -71.0909 -71.0909 42.5495 42.5495 Watershed water collection and stream characterization site for Ipswich River Sylvania Dam, Ipswich, MA -70.837687 -70.837687 42.677543 42.677543 Watershed water collection and stream characterization site for Parker River Central St Dam, Newbury, MA -70.929216 -70.929216 42.750007 42.750007 2001-11-01 2011-07-02 Updated regularly to add dates or to enter data as analysis occurs. Version 01, Sept, 2007. Updated to incorporate most recent data. Version 02, 20 January 2010. Updated to include most recent data. Version 03, February 20, 2011 updated data and metadata, variable names match Version 04, 06Apr2012, data and metadata update. Used MarcrosExportEML_HTML (working)pie_excel2007.xlsm 3/23/2012 12:08 PM for QA/QC to EML 2.1.0 Version 05, 14May2013, metadata and data updated to comply with importation to Drupal and LTER PASTA. Used MarcrosExportEML_HTML (working)pie_excel2007.xlsm 3/14/13 12:02 PM for QA/QC to EML 2.1.0 Data Manager

The Ecosystems Center Marine Biological Lab 7 MBL St Woods Hole MA 02543 USA

(508) 289 7485 pie_im@mbl.edu http://ecosystems.mbl.edu/PIE/ Plum Island Ecosystems LTER

The Ecosystems Center Marine Biological Lab 7 MBL St Woods Hole MA 02543 USA

(508) 289 7485 pie_im@mbl.edu http://ecosystems.mbl.edu/PIE/ Samples were collected with a Sigma auto-sampler on a daily, bi-daily, flow-weighted, or time weighted frequency from each site. Samples were preserved with sulfuric acid, except for those collected by volunteer in winter which were not acidified but frozen within 24 hours. Analysed for nitrate, TP, TN, and TOC. Sigma installation: Sigma autosampler is placed at a convenient location on the bank of each site. Sigma is visited every three weeks to replace/check bottles and batteries. Intake is fastened to reebar suspended above the sediments to prevent intake of particulates from the stream bottom. The bottles contain sulfuric acid to preserve nutrients in their ambient form. Bottles sit out for up to 6 weeks (but most often < 3 weeks) within the sampler before being filled and collected. Occasionally spiders or other bugs are found in the samples. See notes. Daily samples: Sigma pulls a 1L sample the same time each day (usually between 9am to 5pm). TwoDayComposite samples: Sigma pulls a 500ml sample and places it in the same bottle over two consecutive days. (usually grabbed between 9am and 5pm) Volunteer: A grab sample collected by a volunteer during winter period when sigma is not funcitonal. Composite-timeweighted_*hrs - The Sigma pulls a sample every * hours and places it into a single carboy. Usually the volume of the pull is 250ml. The number of hours varied when we first switched to this technique, but is now every 6hrs (4 time per day). Composite-flowweighted_*cms - The Sigma pulls a sample every * cubic meters per second pumped into a single carboy. The amount of cubic meters varies depending on the time of year. This technique relies on a depth sonde attached to the Sigma with associated rating curve. No longer used due to unreliablity of the depth sonde. Samples were processed at the Ecosystems Center in Woods Hole, MA. The processing procedure includes neutralization with sodium hydroxide and sodium bicarbonate, persulfate digestion, and nitrate and phosphate analysis using a Lachat auto-analyzer or spectrophotometer. As of 2004 TN and TOC are run together on a Shimadzu TN/TOC analyzer. Prior to 2004, TN was first digested and then run on a Lachat. TP is still digested and run on a spec. Where samples exist in archive TOC will be run to complement TNs. NOTES AND COMMENTS: Some of the samples from this data set have yet to be run and/or need to be re-run. This file will be updated as samples are analyzed. Specifically, TN and TOC data back to 2004 is still being run to fill in holes. Anne Giblin

The Ecosystems Center Marine Biological Lab 7 MBL St Woods Hole MA 02543 USA

(508) 289 7488 agiblin@mbl.edu Lead PI Hap Garritt

The Ecosystems Center Marine Biological Lab 7 MBL St Woods Hole MA 02543 USA

(508) 289 7485 pie_im@mbl.edu Information Manager The Plum Island Ecosystems (PIE) LTER is an integrated research, education and outreach program whose goal is to develop a predictive understanding of the long-term response of watershed and estuarine ecosystems at the land-sea interface to changes in climate, land use and sea level. The principal study site is the Plum Island Sound estuary, its coupled Parker, Rowley and Ipswich River watersheds and the adjacent coastal ocean, the Gulf of Maine. The PIE LTER focuses on how several aspects of global change influence organic matter and inorganic nutrient biogeochemistry and estuarine foodwebs. The inputs of organic matter and nutrients from land, ocean and marshes interact with the external drivers (climate, land use, river discharge, sea level) to dictate the extent and degree of nutrient and organic matter processing and determine the spatial patterns of estuarine productivity and trophic structure. This material is based upon work supported by the National Science Foundation under Grants OCE-9726921, OCE-0423565. Any opinions, findings, conclusions, or recommendations expressed in the material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation. The Plum Island Ecosystems (PIE) LTER site, is located in northeastern Massachusetts, 42.72 N, 70.85 W. The PIE LTER lies at the interface of a thinly soiled, formerly glaciated New England land mass and the highly productive Gulf of Maine. Three watersheds comprise the estuarine drainage basin: Parker (155 km2), Rowley (26 km2) and Ipswich (404 km2). The Ipswich River watershed is highly urbanized with Boston "bedroom" communities encroaching in the headwater region while the Parker is less urbanized and retains a higher proportion of forest. -71.2198 -70.7568 42.8276 42.4965 0 100 meter WAT-VA-SigmaNutrients.csv Nutrient samples collected by Sigma Autosampler between 2001 and 2011 in three headwater sites of contrasting land use, and at the Parker and Ipswich River Dams as they enter into the estuaries. WAT-VA-SigmaNutrients.csv 1 \r\n column , http://ecosystems.mbl.edu/PIE/data/WAT/data/WAT-VA-SigmaNutrients.csv Site Site Field Collection Site CC Cart Creek (forest) CS Cedar Swamp (wetland) SB Saw Mill Br (urban) ID Ipswich R. Dam PD Parker R. Dam NA NA = not available Year Year Year of collection Year of collection NA NA = not available Date 1 Date 1 Start date of sample collection YYYY-MM-DD NA NA = not available Date 2 Date 2 End date of sample collection YYYY-MM-DD NA NA = not available SampleType SampleType A description of the type of sample routine used with the Sigma Daily one days sample per bottle collected by the autosampler TwoDayComposite two days sample per bottle collected by the autosampler Volunteer one days sample collected by volunteer Composite-timeweighted_*hrs samples are collected every * hours and pumped into a single carboy Composite-flowweighted_*cms samplers are collected after every * cubic meters has passed by the sigma NA NA = not available TP TP Total Phosphorus as P. Digested to Phosphate-P measured with spectrophotometer or Lachat auto-analyzer micromolePerLiter real NA NA = not available TN TN Total Nitrogen as N. Digested to Nitrate + Nitrite and measured with a Lachat auto-analyzer or Shimadzu TN/TOC analyser. micromolePerLiter real NA NA = not available NO3 NO3 Nitrate + Nitrite-N as measured with a Lachat auto-analyzer micromolePerLiter real NA NA = not available TOC TOC Total organic carbon as C. Total organic carbon measured with Shimadzu TN/TOC analyzer. micromolePerLiter real NA NA = not available Notes Notes Comments relevant to quality of the data. 5 Samples were run on Shimadzu and were corrected for poor machine performance so these values are as accurate as possible but are not perfect 6 Samples were run on Shimadzu (see Gaines 2/28/06 processed TN file) and were corrected for poor machine performance so these values are as accurate as possible but are not perfect 7 Samples were collected but have not been analyzed yet 8 Samples will be analyzed for TN/TOC NA NA = not available 4196 µM = µmoles per liter of solution