WAT-YSI-SIGMA-timeseries.03 Wilfred Wollheim

Morse Hall UNH Durham NH 03824 USA

Plum Island Ecosystems LTER Program

The Ecosystems Center Marine Biological Lab 7 MBL St Woods Hole MA 02543 USA

(508) 289 7485 pie_im@mbl.edu http://ecosystems.mbl.edu/PIE/ Brian Pellerin additional investigator Joe Thouin additional investigator Nat Morse additional investigator 2004 Time series of nutrient grab samples collected by hand (i.e. not with the Sigma autosampler) with frequency ranging from weekly to monthly between 2001 and 2009 Sites include three headwater catchments of contrasting land use (forest, urban, wetland), and the mouth of the two main watersheds draining to the Plum Island Estuary (Ipswich and Parker R.). An additional time series was collected for a site in the Upper Ipswich at North Reading and at Fish Br. in Boxford – this sampling was ended in 2002. All Samples were analysed for NO3, NO2, NH4, PO4, TDN and DOC. Si was analyzed until 2002. Particulates and TSS have been analyzed since 2006. PIE LTER inorganic nutrients organic matter catchments Plum Island Massachusetts ammonium dissolved nitrate nitrogen nutrient phosphate phosphorus silicate organic carbon particulates sediment Acceptance and utilization of PIE-LTER data requires that: The Principal Investigator be sent a notice stating reasons for acquiring any data and a description of the publication intentions. The Principal Investigator of the data set be sent a copy of the report or manuscript prior to submission and be adequately cited in any resultant publications A copy of any resultant publications should be sent to: Principal Investigator Ecosystems Center Marine Biological Laboratory Woods Hole, MA 02543 http://ecosystems.mbl.edu/PIE/data/WAT/WAT-YSI-SIGMA-timeseries.html Watershed, YSI-CC (forest) -70.9165 -70.9165 42.7692 42.7692 Watershed, YSI-SB (urban) -71.185 -71.185 42.5236 42.5236 Watershed, YSI-CS (wetland) -71.0909 -71.0909 42.5495 42.5495 Watershed, IP24 (Ipswich Dam, Ipswich) Watershed, 10 (Parker Dam, Byfield) Watershed, YSI-IR (Ipswich at N. Reading) Watershed, YSI-FB (Fish Br. at Boxford) 2001-06-12 2009-08-13 Updated periodically 01) 4Oct2007, EML 4-5 level, 02) 1Dec2007, updated to include recent data 03) 7Jan2010, updated to include recent data Data Manager

The Ecosystems Center Marine Biological Lab 7 MBL St Woods Hole MA 02543 USA

(508) 289 7485 pie_im@mbl.edu http://ecosystems.mbl.edu/PIE/ Plum Island Ecosystems LTER

The Ecosystems Center Marine Biological Lab 7 MBL St Woods Hole MA 02543 USA

(508) 289 7485 pie_im@mbl.edu http://ecosystems.mbl.edu/PIE/ Samples were originally kept cold until filtered within several days of collection, and since 2002 have been filtered immediately in the field. Filtered samples were frozen until analysis. Particulate samples are filtered in the lab using vacuum filtration., and TSS is calculated from the mass of particulates per volume of sample. Particulate C and N mass was calculated by analyzing the filters using a CHN analyzer, and converted to concentration by dividing by the filtered volume. Analysis was conducted at UNH either by the lab of Ted Loder (samples with grid-code = UNH_Grab) at MBL (grid-code = Sigma Grab and Lab_ID = NA) or at UNH by the lab of Bill McDowell (grid-code = Sigma Grab and Lab_ID = McDowell Lab). Samples analyzed at the McDowell Lab below the detection limit were entered as 0. The detection limit is 5 µg/L for NH4 (0.36 µM) , PO4 (0.16 µM) and NO3 (0.36 µM). Samples were analysed using Lachat methods. RESEARCH LOCATION: x and y coordinates given below are in MA State Plane NAD83 (meters). Sites were georeferenced to a gridded river network with 120m grid sizes. X and Y coordinates reflect slight shifts to ensure proper placement on the digital river network. Contributing area is listed in km2 Permanent_ID Site X Site Y Description YSI-SB 225840 919310 Suburban Headwater Stream YSI-CC 247680 946790 Forested Headwater Stream YSI-CS Wetland Headwater Stream YSI-IR 232080 923630 Upper Ipswich R. YSI-FB 240600 934310 Fish Br. IP24 254280 936350 Mouth of the Ipswich (Sylvania Dam) 10 246600 944510 Mouth of the Parker (Central St.) Anne Giblin

The Ecosystems Center Marine Biological Lab 7 MBL St Woods Hole MA 02543 USA

(508) 289 7488 agiblin@mbl.edu Lead PI Hap Garritt

The Ecosystems Center Marine Biological Lab 7 MBL St Woods Hole MA 02543 USA

(508) 289 7485 pie_im@mbl.edu Information Manager The Plum Island Ecosystems (PIE) LTER is an integrated research, education and outreach program whose goal is to develop a predictive understanding of the long-term response of watershed and estuarine ecosystems at the land-sea interface to changes in climate, land use and sea level. The principal study site is the Plum Island Sound estuary, its coupled Parker, Rowley and Ipswich River watersheds and the adjacent coastal ocean, the Gulf of Maine. The PIE LTER focuses on how several aspects of global change influence organic matter and inorganic nutrient biogeochemistry and estuarine foodwebs. The inputs of organic matter and nutrients from land, ocean and marshes interact with the external drivers (climate, land use, river discharge, sea level) to dictate the extent and degree of nutrient and organic matter processing and determine the spatial patterns of estuarine productivity and trophic structure. This material is based upon work supported by the National Science Foundation under Grants OCE-9726921, OCE-0423565. Any opinions, findings, conclusions, or recommendations expressed in the material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation. The Plum Island Ecosystems (PIE) LTER site, is located in northeastern Massachusetts, 42.72 N, 70.85 W. The PIE LTER lies at the interface of a thinly soiled, formerly glaciated New England land mass and the highly productive Gulf of Maine. Three watersheds comprise the estuarine drainage basin: Parker (155 km2), Rowley (26 km2) and Ipswich (404 km2). The Ipswich River watershed is highly urbanized with Boston "bedroom" communities encroaching in the headwater region while the Parker is less urbanized and retains a higher proportion of forest. -71.2198 -70.7568 42.8276 42.4965 0 100 meter uid=PIE,o=lter,dc=ecoinformatics,dc=org all public read WAT-YSI-SIGMA-timeseries.dat Time series of nutrient grab samples from catchments with frequency ranging from weekly to monthly between 2001 and 2009. WAT-YSI-SIGMA-timeseries.dat 1 column , http://ecosystems.mbl.edu/PIE/data/WAT/data/WAT-YSI-SIGMA-timeseries.dat Permanent_ID Permanent_ID station ID YSI-CC Cart Creek (forest) YS-CS Cedar Swamp (wetland) YSI-SB Saw Mill Br (urban) IP24 Ipswich R. Dam Ipswich 10 Parker R. Dam Byfield YSI-IR upper Ipswich R. in N. Reading YSI-FB Fish Br. In Boxford SampleType SampleType Identifies the type of sample collected UNH-GRAB Grab sample collected by UNH prior to 2003 Sigma-GRAB Grab sample collected by either MBL (prior to 2004) or UNH (after 2004) Lab_ID Lab_ID ID used to track sample through lab PK* unique sample identifier for samples analysed in the Loder Lab at UNH McDowellLab_YYYY analysis at the McDowell Lab in year YYYY NA sample analysed at MBL prior to 2003 DATE DATE date (yyyy-mm-dd) yyyy-mm-dd NO3 NO3 nitrate + nitrite-N (µM-N) microMolesPerLiter real NH4 NH4 ammonium-N (µM-N) microMolesPerLiter real PO4 PO4 soluble reactive phosphorus (µM-P) microMolesPerLiter real NO2 NO2 nitrite-N (µM-N) microMolesPerLiter real Si Si dissolved silica (µM-S) microMolesPerLiter real TDN TDN total dissolved nitrogen (µM-N) microMolesPerLiter real DOC DOC dissolved organic carbon (µM-C) microMolesPerLiter real PON PON particulate organic nitrogen (µM-N) microMolesPerLiter real POC POC particulate organic carbon (µM-C) microMolesPerLiter real TSS TSS Total Suspended Solids (mg/L) milligramsPerLiter real µM = µmoles per liter of solution