http://ecosystems.mbl.edu/pie

Acceptance and utilization of PIE-LTER data requires that:

• The Principal Investigator be sent a notice stating reasons for acquiring any data and a description of the publication intentions.
• The Principal Investigator of the data set be sent a copy of the report or manuscript prior to submission and be adequately cited in any resultant publications.
• A copy of any resultant publications should be sent to:

(Principal Investigator)
Ecosystems Center;
Marine Biological Laboratory
Woods Hole, MA 02543

PLUM ISLAND ECOSYSTEM LTER DATABASE

DATA FILE: STP-PR-Mesocosm.txt

DOCUMENTATION FILE: STP-PR-Mesocosm.doc

YEAR:

PI: Charles Hopkinson

OTHERS: Linda Deegan, Anne Giblin, John Hobbie, Joseph Vallino, Ishi Buffam, Robert Garritt, Jane Tucker, Meredith Hullar, Mark Benfield, and Ee Lin Lim

BRIEF DESCRIPTION OF DATA FILE: Mesocosm experiment in which the effects of dissolved organic matter and nutrient additions on estuarine food webs were investigated.

KEYWORDS: mesocosm, trophic, food web, light, ammonium, nitrate, phosphate, organic, carbon, nitrogen, respiration, production, community, fish, isotope, bacteria, chlorophyll

SITE TYPE: Short-term Projects and Experiments

RESEARCH LOCATION: Parker River Estuary

EXPERIMENTAL DESIGN AND METHODS:

In the Woods Hole Harbor, MA, four large transparent bags (7m³) were submerged and filled with sea-water. Twelve larval Atlantic silversides (Menidia menidia) were added to each bag. Large fish and other predators were excluded from the mesocosms.

The treatments consisted of a control, an organic matter addition, a daily nutrient addition, and organic matter plus nutrient addition. Organic matter additions were 35 mM dissolved organic carbon (GF/F filtered), from leached leaf litter, and was added once at the outset of the experiment. 5 mM NO₃^-, 0.5 mM PO₄^3-, and 7 uM SiO₃ were added daily (at 17:00) in the nutrient addition bags. All mesocosms also received NaH¹³CO₃ to raise d ¹³C of DIC pool to 120 ‰.

NOTES AND COMMENTS:

VARIABLE DESCRIPTION:

DAY = day from start of experiment

TREATMENT = treatment (CONTROL = control; DOM = dissolved organic matter addition; NUTS = nutrient addition; DOM+NUTS = dissolved organic matter and nutrient addition)

CATEGORY = category of data (WATER CHEMISTRY = NH4, NO3, PO4, Chlorophyll, DOC, POC, PON; PRODUCTION = bacterial production, primary production, size fractionated production; METABOLISM = gross production, respiration, net community production; LIGHT EXTINCTION = light extinction; FISH = fish weight and isotope data, ISOTOPE = isotope data for various dissolved species, particulate size fractions and fish; ZOOPLANKTON = zooplankton species counts; DIATOM = diatom species counts; µFLAGELLATES = µflagellate counts; CILIATES = ciliate species counts, DINOFLAGELLATES = dinoflagellate species counts; BACTERIA = bacterial counts; PAR = light data

TYPE = further description of data type

SIZE MIN = minimum size of sample (µm)

SIZE MAX = maximum size of sample (µm)

NUMBER = number of organisms per Liter of sample

NH4 = ammonium (µM)

NH4 SE = standard error of ammonium analysis

PO4 = soluble reactive phosphorus (µM)

NO3 = nitrate + nitrite (µM)

DOC = dissolved organic carbon (µM)

POC = particulate organic carbon (µM)

PON = particulate organic nitrogen (µM)

CHLA = chlorophyll a (µg L^-1)

PP = primary production (µmol C L^-1 d^-1)

GP = gross production (µmol C mesocosm^-1 d^-1)

RESP = respiration (µmol C mesocosm^-1 d^-1)

NCP = net community production (µmol C mesocosm^-1 d^-1)

BAC PROD = bacterial production (µmol C d^-1)

LTEXT = light extinction coefficient (m^-1)

FISH WT = fish weight (g fish^-1)

15N = d ¹⁵N of sample (per mil)

13C = d ¹³C of sample (per mil)

PAR = photosynethetically active radiation (µE m^-2 s^-1)

CALCULATIONS:

FOR MORE INFORMATION, CONTACT:

OTHER DATA FILES TO REFERENCE:

REFERENCE CITATIONS:

DATA AVAILABILITY STATUS:

Type 3

ARCHIVE INFORMATION:

Date of entry:

Data entered by:

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