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Conditions of Use

The re-use of scientific data has the potential to greatly increase communication, collaboration and synthesis within and among disciplines, and thus is fostered, supported and encouraged. Permission to use this dataset is granted to the Data User free of charge subject to the following terms:

1) Acceptable use. Use of the dataset will be restricted to academic, research, educational, government, recreational, or other not-for-profit professional purposes. The Data User is permitted to produce and distribute derived works from this dataset provided that they are released under the same license terms as those accompanying this Data Set. Any other uses for the Data Set or its derived products will require explicit permission from the dataset owner.
2 ) Redistribution. The data are provided for use by the Data User. The metadata and this license must accompany all copies made and be available to all users of this Data Set. The Data User will not redistribute the original Data Set beyond this collaboration sphere.
3 ) Citation. It is considered a matter of professional ethics to acknowledge the work of other scientists. Thus, the Data User will properly cite the Data Set in any publications or in the metadata of any derived data products that were produced using the Data Set. Citation should take the following general form: Creator, Year of Data Publication, Title of Dataset, Publisher, Dataset identifier. For example:

Shaver, G. 1989. Above ground biomass in acidic tussock tundra experimental site, 1989, Arctic LTER, Toolik, Alaska. Arctic LTER, Marine Biological Lab, Woods Hole, Ma 02543. 1989gsttbm http://ecosystems.mbl.edu/arc/terrest/biomass/index.shtml 

4 ) Acknowledgement. The Data User should acknowledge any institutional support or specific funding awards referenced in the metadata accompanying this dataset in any publications where the Data Set contributed significantly to its content. Acknowledgements should identify the supporting party, the party that received the support, and any identifying information such as grant numbers. For example:

Data sets were provided by the Arctic LTER. This material is based upon work supported by the National Science Foundation under Grants #DEB-981022, 9211775, 8702328; #OPP-9911278, 9911681, 9732281, 9615411, 9615563, 9615942, 9615949, 9400722, 9415411, 9318529; #BSR 9019055, 8806635, 8507493.

5 ) Notification. The Data User will notify the Data Set Contact when any derivative work or publication based on or derived from the Data Set is distributed. The Data User will provide the data contact with two reprints of any publications resulting from use of the Data Set and will provide copies, or on-line access to, any derived digital products. Notification will include an explanation of how the Data Set was used to produce the derived work.
6 ) Collaboration. The Data Set has been released in the spirit of open scientific collaboration. Data Users are thus strongly encouraged to consider consultation, collaboration and/or co-authorship with the Data Set Creator.

By accepting this Data Set, the Data User agrees to abide by the terms of this agreement. The Data Owner shall have the right to terminate this agreement immediately by written notice upon the Data User's breach of, or non-compliance with, any of its terms. The Data User may be held responsible for any misuse that is caused or encouraged by the Data User's failure to abide by the terms of this agreement.

Disclaimer

While substantial efforts are made to ensure the accuracy of data and documentation contained in this Data Set, complete accuracy of data and metadata cannot be guaranteed. All data and metadata are made available "as is". The Data User holds all parties involved in the production or distribution of the Data Set harmless for damages resulting from its use or interpretation.

Dataset URLs:METADATA: HTML, Rich Text, XML(EML compliant)
DATA: Comma Delimited, Excel file with Metadata and data, Dataset via LTER Data Poral
Dataset ID:1992-2000hobact.03
Dataset Title:Number of bacteria in the water column of lakes sampled near Toolik Lake LTER Alaska, throughout summer season, 1992-2000.
Investigator 1: 
First Name:John
Last Name:Hobbie
Organization:Marine Biological Laboratory
Address line 2:The Ecosystems Center
Address line 3:
City:Woods Hole
State:MA
Zip Code:02540
Country:USA
Investigator 2: 
First Name:Michele
Last Name:Bahr
Organization:
Address line 2:
City:
State:
Zip Code:
Country:
Investigator 3: 
First Name:Byron
Last Name:Crump
Investigator 4: 
First Name:Anne
Last Name:Burns
Organization:
Address line 2:
City:
State:
Zip Code:
Country:
Associate Investigators:
Keywords:bacteria, picoplankton, lakes, Alaska, arctic lakes
Abstract:Number of bacteria (using of DAPI for identifying and counting) in Toolik Lake water column and other lakes sample throught the summer from 1992-2000. There is no data for 2006.
For questions about the Metadata and data contact the Investigators.
For information about this web site contact:
Arctic LTER Information Manager
The Ecosystems Center
Marine Biological Lab
7 MBL St
Woods Hole, MA 02543
Phone (508) 289 7496
Email: arc_im@mbl.edu
Online URL: http://ecosystems.mbl.edu/ARC/
DATA FILE INFORMATION:
Data File URL http://metacat.lternet.edu/das/dataAccessServlet?docid=knb-lter-arc.10301&urlTail=lakes/Bacteria/data/1992-2000hobact.csv
Data File Name 1992-2000hobact
Beginning Date 5/1/1992
End Date 9/1/2000
Number of Data Records 269
Other Files to Reference
Availability Status
Quality Control Information
Maintenance Description
Log of Changes:
Version 1: Combined all the years from 92hobact to 2000hobact files. JimL 29May13
Version 2: added all the researchers from 1992-2000; and all the sites. JimL 9Jun13
Version 3: Checked keywords against the LTER network preferred list and replaced non-preferred terms. Jim L 15Jan14
 
RESEARCH LOCATION:                                            
Location Name Toolik N 02 Toolik Inlet Camp Pond Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One  
Geographic Description Toolik Lake Lake N2 Main inlet to Toolik Lake Near old camp by the south end of the runway Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description  
Location Bounding Box                                            
West Bounding Coordinate                                            
East Bounding Coordinate                                            
North Bounding Coordinate                                            
South Bounding Coordinate                                            
OR if single point location                                            
Latitude 68.629961 68.6409472345 68.6256 68.6333333333333 In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees  
Longitude -149.612633 -149.625086307 -149.59605 -149.6 In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees  
Elevation 719 724 719 720 In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters  
Link to Google Map View on Google Map View on Google Map View on Google Map View on Google Map                                    
                                             
 
TAXONOMIC COVERAGE:
Organisms studied
 
Methods:For years 1992 -1994

2 ml of gluteraldehyde-preserved lake water was stained with 200 ul 100ug/ml
4'6 diamidino-2-phenylindole (DAPI), final concentration 10 ug/ml, in a 15 ml
Millipore filtration apparatus. After 5 minutes, the sample was vacuum filtered
through a 0.22 um pore size black Nuclepore 25mm filter. The filter was mounted
on a microscope slide using Cargille B immersion oil. Ten fields were counted
using a Zeiss epifluoresencce microscope.

Cell count= (x/field)(2.04 x 10^4 field/ml) = #/ml

For years 1995-2000

After transport to the laboratory at Woods Hole, MA, 2 ml of glutaraldehyde-
preserved lake water from each depth was mixed with 2 ml 0.2 um filtered
phosphate buffered saline to reduce humic interference and 0.1% Triton X-100 or
Nonidet P-40 to improve cell permeability and stored overnight at 4*C. The
sample was stained with 200 ul 100ug/ml 4'6 diamidino-2-phenylindole (DAPI),
final concentration 10 ug/ml. After 5 minutes, the sample was vacuum filtered
through a 0.22 um pore size black Nuclepore 25mm filter in a 15 ml Millipore
filtration apparatus. The filter was mounted on a microscope slide using
Cargille A immersion oil. Ten fields were counted using a Zeiss epifluoresencce
microscope.

Reference Citations: Hobbie, J.E., T.L. Corliss and B.J. Peterson, Seasonal patterns of bacterial
abundance in an
Arctic lake. 1983. Arctic and Alpine Research 15:252-259.
Porter, K.G. and Y.S.Feig The use of DAPI for identifying and counting aquatic microflora.
1980. Limnology and Oceanography 25:943-948

Data Table

Variable Name Variable Description Data Type Units DateTime Format Code Information Missing Value Code
Site Code Site code (LTER code) text       .=Missing or Not Measured
Site Site of measurement text       .=Missing or Not Measured
Station Station text       .=Missing or Not Measured
Depth Depth in meters number meter     .=Missing or Not Measured
Date Date datetime   DD-MMM-YYYY   .=Missing or Not Measured
Count cell count= # cells x 10^6/ml number number     .=Missing or Not Measured
Comments Notes about data text       .=Missing or Not Measured

Please contact arc_im@mbl.edu with questions, comments, or for technical assistance regarding this web site.