Weather at Toolik

Arctic LTER Database

Acceptance and utilization of LTER data requires that:

(1) The Principal Investigator be sent a notice stating reasons for acquiring any data and a description of the publication intentions.
(2) The Principal Investigator of the data set be sent a copy of the report or manuscript prior to submission and be adequately cited in any resultant publications.
(3) A copy of any resultant publications should be sent to:

Principal Investigator
Ecosystems Center
Marine Biological Laboratory
7 MBL St.
Woods Hole, MA 02543

Older Metadata: Attribute table not in XML (EML) file

Dataset URLs:	METADATA: HTML, Rich Text, XML(EML compliant) DATA: Comma Delimited, Excel file with Metadata and data
Dataset ID:	98hobact
Dataset Title:	Number of bacteria in Toolik Lake water column during May, June, July and August 1998 , Arctic LTER 1998.
Investigator 1:
First Name:	John
Last Name:	Hobbie
Address line 1:
Address line 2:
Address line 3:
City:
State:
Zip Code:
Country:
Associate Investigators:	Michele Bahr
Keywords:	bacteria, picoplankton
Abstract:	Number of bacteria in Toolik Lake water column during May, June, July and August 1998
Contact:	Arctic LTER Information Manager The Ecosystems Center Marine Biological Lab 7 MBL St Woods Hole, MA 02543 Phone (508) 289 7496 Email: arc_im@mbl.edu Online URL: http://ecosystems.mbl.edu/ARC/
DATA FILE INFORMATION:
Data File Name	98hobact
Beginning Date	5/1/1998
End Date	9/1/1998
Number of Data Records
Other Files to Reference
Availability Status
Quality Control Information
Maintenance Description
Log of Changes:	Log of changes: For Archival Use: DATE RECEIVED: Feb 22, 2001 DATA FILE ENTERED BY: DATA FILE VALIDATION: NAME: Neil Bettez DATE:



RESEARCH LOCATION:
Geographic Description	Toolik Lake, Camp Pond
Location Bounding Box
West Bounding Coordinate
East Bounding Coordinate
North Bounding Coordinate
South Bounding Coordinate
OR if single point location
Latitude
Longitude



TAXONOMIC COVERAGE:
Organisms studied

Methods:	After transport to the laboratory at Woods Hole, MA, 2 ml of glutaraldehyde-preserved lake water from each depth was mixed with 2 ml 0.2 um filtered phosphate buffered saline to reduce humic interference and 0.1% Triton X-100 or Nonidet P-40 to improve cell permeability and stored overnight at 4*C. The sample was stained with 200 ul 100ug/ml 4'6 diamidino-2-phenylindole (DAPI), final concentration 10 ug/ml. After 5 minutes, the sample was vacuum filtered through a 0.22 um pore size black Nuclepore 25mm filter in a 15 ml Millipore filtration apparatus. The filter was mounted on a microscope slide using Cargille A immersion oil. Ten fields were counted using a Zeiss epifluoresencce microscope. CALCULATIONS Variable Formula cell count (x/field)(2.04 x 10^4 field/ml) = #/ml Reference Citations: Hobbie, J.E., T.L. Corliss and B.J. Peterson, Seasonal patterns of bacterial abundance in an Arctic lake. 1983. Arctic and Alpine Research 15:252-259. Porter, K.G. and Y.S.Feig The use of DAPI for identifying and counting aquatic microflora. 1980. Limnology and Oceanography 25:943-948. Sampling Description. None

Data Table

Variable    Variable descrip.            Precis./Units  Coded   Missing
SITE        site of measurement                           y
DATE        Date                           DD-MMM-YY      n
STATION    location at lake
DEPTH       meters
COUNT      # cells x 10^6/ml             0.00