Weather at Toolik

Arctic LTER Database

Acceptance and utilization of LTER data requires that:

(1) The Principal Investigator be sent a notice stating reasons for acquiring any data and a description of the publication intentions.
(2) The Principal Investigator of the data set be sent a copy of the report or manuscript prior to submission and be adequately cited in any resultant publications.
(3) A copy of any resultant publications should be sent to:

Principal Investigator
Ecosystems Center
Marine Biological Laboratory
7 MBL St.
Woods Hole, MA 02543

Older Metadata: Attribute table not in XML (EML) file

Dataset URLs:	METADATA: HTML, Rich Text, XML(EML compliant) DATA: Comma Delimited, Excel file with Metadata and data
Dataset ID:	99hobact
Dataset Title:	Number of bacteria in Toolik Lake water column during May and June 1999, and bacteria in Camp Pond in July and Aug, Arctic LTER 1999.
Investigator 1:
First Name:	John
Last Name:	Hobbie
Address line 1:
Address line 2:
Address line 3:
City:
State:
Zip Code:
Country:
Associate Investigators:	Michele Bahr, Anne Burns
Keywords:	bacteria, picoplankton
Abstract:	Number of bacteria in Toolik Lake water column during May and June 1999, and bacteria in Camp Pond in July and Aug. 1999.
Contact:	Arctic LTER Information Manager The Ecosystems Center Marine Biological Lab 7 MBL St Woods Hole, MA 02543 Phone (508) 289 7496 Email: arc_im@mbl.edu Online URL: http://ecosystems.mbl.edu/ARC/
DATA FILE INFORMATION:
Data File Name	99hobact
Beginning Date	5/1/1999
End Date	9/1/1999
Number of Data Records
Other Files to Reference
Availability Status
Quality Control Information
Maintenance Description
Log of Changes:	Log of changes: For Archival Use: DATE RECEIVED: Feb 22, 2001 DATA FILE ENTERED BY: DATA FILE VALIDATION: NAME: Neil Bettez DATE:



RESEARCH LOCATION:
Geographic Description	Toolik Lake and Camp Pond
Location Bounding Box
West Bounding Coordinate
East Bounding Coordinate
North Bounding Coordinate
South Bounding Coordinate
OR if single point location
Latitude
Longitude



TAXONOMIC COVERAGE:
Organisms studied

Methods:	After transport to the laboratory at Woods Hole, MA, 2 ml of glutaraldehyde- preserved lake water from each depth was mixed with 2 ml 0.2 um filtered phosphate buffered saline to reduce humic interference and 0.1% Triton X-100 or Nonidet P-40 to improve cell permeability and stored overnight at 4*C. The sample was stained with 200 ul 100ug/ml 4'6 diamidino-2-phenylindole (DAPI), final concentration 10 ug/ml. After 5 minutes, the sample was vacuum filtered through a 0.22 um pore size black Nuclepore 25mm filter in a 15 ml Millipore filtration apparatus. The filter was mounted on a microscope slide using Cargille A immersion oil. Ten fields were counted using a Zeiss epifluoresencce microscope. CALCULATIONS Variable Formula cell count (x/field)(2.04 x 10^4 field/ml) = #/ml Reference Citations: Hobbie, J.E., T.L. Corliss and B.J. Peterson, Seasonal patterns of bacterial abundance in an Arctic lake. 1983. Arctic and Alpine Research 15:252-259. Porter, K.G. and Y.S.Feig The use of DAPI for identifying and counting aquatic microflora. 1980. Limnology and Oceanography 25:943-948. Sampling Description. None

Data Table

Variable    Variable descrip.            Precis./Units  Coded   Missing
(Y/N)    Values
SITE        site of measurement                           y
DATE        Date                           DD-MMM-YY      n
STATION   location at lake
DEPTH       meters
COUNT      # cells x 10^6/ml             0.00