Arctic LTER

Weather at Toolik

Arctic LTER Database

Conditions of Use

The re-use of scientific data has the potential to greatly increase communication, collaboration and synthesis within and among disciplines, and thus is fostered, supported and encouraged. Permission to use this dataset is granted to the Data User free of charge subject to the following terms:

1) Acceptable use. Use of the dataset will be restricted to academic, research, educational, government, recreational, or other not-for-profit professional purposes. The Data User is permitted to produce and distribute derived works from this dataset provided that they are released under the same license terms as those accompanying this Data Set. Any other uses for the Data Set or its derived products will require explicit permission from the dataset owner.
2 ) Redistribution. The data are provided for use by the Data User. The metadata and this license must accompany all copies made and be available to all users of this Data Set. The Data User will not redistribute the original Data Set beyond this collaboration sphere.
3 ) Citation. It is considered a matter of professional ethics to acknowledge the work of other scientists. Thus, the Data User will properly cite the Data Set in any publications or in the metadata of any derived data products that were produced using the Data Set. Citation should take the following general form: Creator, Year of Data Publication, Title of Dataset, Publisher, Dataset identifier. For example:

Shaver, G. 1989. Above ground biomass in acidic tussock tundra experimental site, 1989, Arctic LTER, Toolik, Alaska. Arctic LTER, Marine Biological Lab, Woods Hole, Ma 02543. 1989gsttbm http://ecosystems.mbl.edu/arc/terrest/biomass/index.shtml

4 ) Acknowledgement. The Data User should acknowledge any institutional support or specific funding awards referenced in the metadata accompanying this dataset in any publications where the Data Set contributed significantly to its content. Acknowledgements should identify the supporting party, the party that received the support, and any identifying information such as grant numbers. For example:

Data sets were provided by the Arctic LTER. This material is based upon work supported by the National Science Foundation under Grants #DEB-981022, 9211775, 8702328; #OPP-9911278, 9911681, 9732281, 9615411, 9615563, 9615942, 9615949, 9400722, 9415411, 9318529; #BSR 9019055, 8806635, 8507493.

5 ) Notification. The Data User will notify the Data Set Contact when any derivative work or publication based on or derived from the Data Set is distributed. The Data User will provide the data contact with two reprints of any publications resulting from use of the Data Set and will provide copies, or on-line access to, any derived digital products. Notification will include an explanation of how the Data Set was used to produce the derived work.
6 ) Collaboration. The Data Set has been released in the spirit of open scientific collaboration. Data Users are thus strongly encouraged to consider consultation, collaboration and/or co-authorship with the Data Set Creator.

By accepting this Data Set, the Data User agrees to abide by the terms of this agreement. The Data Owner shall have the right to terminate this agreement immediately by written notice upon the Data User's breach of, or non-compliance with, any of its terms. The Data User may be held responsible for any misuse that is caused or encouraged by the Data User's failure to abide by the terms of this agreement.

Disclaimer

While substantial efforts are made to ensure the accuracy of data and documentation contained in this Data Set, complete accuracy of data and metadata cannot be guaranteed. All data and metadata are made available "as is". The Data User holds all parties involved in the production or distribution of the Data Set harmless for damages resulting from its use or interpretation.

Dataset URLs:	METADATA: HTML, Rich Text, XML(EML compliant) DATA: Comma Delimited, Excel file with Metadata and data
Dataset ID:	95hobact.3
Dataset Title:	Number of bacteria in the water column of lakes sampled near Toolik Lake LTER throughout summer season, 1995.
Investigator 1:
First Name:	John
Last Name:	Hobbie
Organization:	Marine Biological Laboratory
Address line 2:	The Ecosystems Center
Address line 3:
City:	Woods Hole
State:	MA
Zip Code:	02540
Country:	USA
Investigator 2:
First Name:	Michele
Last Name:	Bahr
Organization:
Address line 2:
City:
State:
Zip Code:
Country:
Associate Investigators:
Keywords:	bacteria, picoplankton
Abstract:	Number of bacteria in Toolik Lake water column during June, July and August 1995
For questions about the Metadata and data contact the Investigators. For information about this web site contact:	Arctic LTER Information Manager The Ecosystems Center Marine Biological Lab 7 MBL St Woods Hole, MA 02543 Phone (508) 289 7496 Email: arc_im@mbl.edu Online URL: http://ecosystems.mbl.edu/ARC/
DATA FILE INFORMATION:
Data File URL	http://metacat.lternet.edu/das/dataAccessServlet?docid=knb-lter-arc.966&urlTail=lakes/plankton/data/95hobact.csv
Data File Name	95hobact.xls
Beginning Date	5/1/1995
End Date	9/1/1995
Number of Data Records	39
Other Files to Reference
Availability Status
Quality Control Information
Maintenance Description
Log of Changes:	Log of changes: For Archival Use: DATE RECEIVED: Feb 22, 1996 DATA FILE ENTERED BY: DATA FILE VALIDATION: NAME: Neil Bettez DATE:
	Version 3: Metadata updated to new version (with sites sheet). Data columns added to match other years. CH April 2013.

RESEARCH LOCATION:

Location Name

Toolik

Select Site or enter New One

Geographic Description

Toolik Lake

Enter Description

Location Bounding Box

West Bounding Coordinate

East Bounding Coordinate

North Bounding Coordinate

South Bounding Coordinate

OR if single point location

Latitude

68.629961

In Decimal Degrees

Longitude

-149.612633

In Decimal Degrees

Elevation

719

In Meters

Link to Google Map

View on Google Map


TAXONOMIC COVERAGE:
Organisms studied

Methods:	After transport to the laboratory at Woods Hole, MA, 2 ml of gluteraldehyde-preserved lake water from each depth was mixed with 2 ml 0.2 um filtered phosphate buffered saline to reduce humic interference and 0.1% Triton X-100 or Nonidet P-40 to improve cell permeability and stored overnight at 4*C. The sample was stained with 200 ul 100ug/ml 4'6 diamidino-2-phenylindole (DAPI), final concentration 10 ug/ml. After 5 minutes, the sample was vacuum filtered through a 0.22 um pore size black Nuclepore 25mm filter in a 15 ml Millipore filtration apparatus. The filter was mounted on a microscope slide using Cargille A immersion oil. Ten fields were counted using a Zeiss epifluoresencce microscope. Reference Citations: Hobbie, J.E., T.L. Corliss and B.J. Peterson, Seasonal patterns of bacterial abundance in an Arctic lake. 1983. Arctic and Alpine Research 15:252-259. Porter, K.G. and Y.S.Feig The use of DAPI for identifying and counting aquatic microflora. 1980. Limnology and Oceanography 25:943-948. Sampling Description. None

Data Table

Variable Name	Variable Description	Data Type	Units	DateTime Format	Code Information	Missing Value Code
Site Code	Site code (LTER code)	text				.=Missing or Not Measured
Site	Site of measurement	text				.=Missing or Not Measured
Station	Station	text				.=Missing or Not Measured
Depth	Depth in meters	number	meter			.=Missing or Not Measured
Date	Date	datetime		DDMMMYYYY		.=Missing or Not Measured
Count	cell count= # cells x 10^6/ml	number	number			.=Missing or Not Measured
Comments	Notes about data	text				.=Missing or Not Measured

	Please contact arc_im@mbl.edu with questions, comments, or for technical assistance regarding this web site.