Arctic LTER Database

Conditions of Use

The re-use of scientific data has the potential to greatly increase communication, collaboration and synthesis within and among disciplines, and thus is fostered, supported and encouraged. Permission to use this dataset is granted to the Data User free of charge subject to the following terms:

1) Acceptable use. Use of the dataset will be restricted to academic, research, educational, government, recreational, or other not-for-profit professional purposes. The Data User is permitted to produce and distribute derived works from this dataset provided that they are released under the same license terms as those accompanying this Data Set. Any other uses for the Data Set or its derived products will require explicit permission from the dataset owner.
2 ) Redistribution. The data are provided for use by the Data User. The metadata and this license must accompany all copies made and be available to all users of this Data Set. The Data User will not redistribute the original Data Set beyond this collaboration sphere.
3 ) Citation. It is considered a matter of professional ethics to acknowledge the work of other scientists. Thus, the Data User will properly cite the Data Set in any publications or in the metadata of any derived data products that were produced using the Data Set. Citation should take the following general form: Creator, Year of Data Publication, Title of Dataset, Publisher, Dataset identifier. For example:

Shaver, G. 1989. Above ground biomass in acidic tussock tundra experimental site, 1989, Arctic LTER, Toolik, Alaska. Arctic LTER, Marine Biological Lab, Woods Hole, Ma 02543. 1989gsttbm http://ecosystems.mbl.edu/arc/terrest/biomass/index.shtml 

4 ) Acknowledgement. The Data User should acknowledge any institutional support or specific funding awards referenced in the metadata accompanying this dataset in any publications where the Data Set contributed significantly to its content. Acknowledgements should identify the supporting party, the party that received the support, and any identifying information such as grant numbers. For example:

Data sets were provided by the Arctic LTER. This material is based upon work supported by the National Science Foundation under Grants #DEB-981022, 9211775, 8702328; #OPP-9911278, 9911681, 9732281, 9615411, 9615563, 9615942, 9615949, 9400722, 9415411, 9318529; #BSR 9019055, 8806635, 8507493.

5 ) Notification. The Data User will notify the Data Set Contact when any derivative work or publication based on or derived from the Data Set is distributed. The Data User will provide the data contact with two reprints of any publications resulting from use of the Data Set and will provide copies, or on-line access to, any derived digital products. Notification will include an explanation of how the Data Set was used to produce the derived work.
6 ) Collaboration. The Data Set has been released in the spirit of open scientific collaboration. Data Users are thus strongly encouraged to consider consultation, collaboration and/or co-authorship with the Data Set Creator.

By accepting this Data Set, the Data User agrees to abide by the terms of this agreement. The Data Owner shall have the right to terminate this agreement immediately by written notice upon the Data User's breach of, or non-compliance with, any of its terms. The Data User may be held responsible for any misuse that is caused or encouraged by the Data User's failure to abide by the terms of this agreement.

Disclaimer

While substantial efforts are made to ensure the accuracy of data and documentation contained in this Data Set, complete accuracy of data and metadata cannot be guaranteed. All data and metadata are made available "as is". The Data User holds all parties involved in the production or distribution of the Data Set harmless for damages resulting from its use or interpretation.

Dataset URLs:	METADATA: HTML, Rich Text, XML(EML compliant) DATA: Comma Delimited, Excel file with Metadata and data
Dataset ID:	2006_Chla_Kling.07
Dataset Title:	Chlorophyl a Data for lakes and lake inlets/outlets samples collected summer 2006, Arctic LTER, Toolik Research Station, Alaska.
Investigator 1:
First Name:	George
Last Name:	Kling
Organization:	University of Michigan
Address line 2:	Department of Ecology and Evolutionary Biology
Address line 3:	830 North University
City:	Ann Arbor
State:	MI
Zip Code:	48109-1048
Country:	United States of America
Investigator 2:
First Name:	MaryAnne
Last Name:	Evans
Organization:
Address line 2:
Address line 3:
City:
State:
Zip Code:
Country:
Investigator 3:
First Name:	Amanda
Last Name:	Field
Associate Investigators:
Keywords:	phytoplankton, Chlorophyll a, phaeophytin, lake, pelagic, stream, primary production.
Abstract:	Yearly file containing information on Chlorophyll a determinations. Samples were collected at various sites near Toolik Research Station (68 38'N, 149 36'W). Sample site descriptors include an assigned number (sortchem), site, date, time, depth, distance, treatment, and category. Data regarding chlorophyll a and phaeopigment concentrations and parameters of sample analysis are included.
For questions about the Metadata and data contact the Investigators. For information about this web site contact:	Arctic LTER Information Manager The Ecosystems Center Marine Biological Lab 7 MBL St Woods Hole, MA 02543 Phone (508) 289 7496 Email: arc_im@mbl.edu Online URL: http://ecosystems.mbl.edu/ARC/
DATA FILE INFORMATION:
Data File URL	http://metacat.lternet.edu/das/dataAccessServlet?docid=knb-lter-arc.1650&urlTail=landwater/chlorophyll/data/2006_Chla_Kling.csv
Data File Name	2006_Chla_Kling.dat
Beginning Date	5/18/2006
End Date	8/10/2006
Number of Data Records	163
Other Files to Reference
Availability Status
Quality Control Information
Maintenance Description
Log of Changes:	Entered metadata into new metadata form.
	Version 6: Update LTERNET Data Access server proxy link for Excel and comma delimited data files. Changed from knb to das in url.
	Version 7: Updated metadata form to newer version (without site sheet). CH 4Feb2013. Updated metadata to newer form (with sites sheet). CH March 2013.

RESEARCH LOCATION:
Location Name	Toolik	Campsite lake	Green Cabin lake	I minus	I minus 01	I minus 02	I Swamp Inlet	I Swamp Outlet	I1 into I3	I1 Outlet	I2 into I3	I2 Outlet	I3 Outlet	I4 into I5	I4 Outlet	I5 into I6	I5 Outlet	I6 headwater lake inlet	I6 headwater lake outlet	I6 Inlet West	I6 Outlet	I7 into I9	I7 Outlet	I8 Headwater	I8 Inlet	I8 into I9	I8 Outlet	Island Lake	Milkyway upper	Milkyway Lower	NE 14 lake	NE 14 lake slump inlet	Toolik central	Toolik Inlet	TW Weir
Geographic Description	Toolik Lake and vicinity. See http://ecosystems.mbl.edu/arc/landwater/lw_sitedescript.html	Campsite lake	Headwater Lake of the Kupurak River	I minus	I minus 01	I minus 02	Swamp between I8 an I9	I Swamp Outlet	I1 into I3	I1 Outlet	I2 into I3	I2 Outlet	I3 Outlet	I4 into I5	I4 Outlet	I5 into I6	I5 Outlet	I6 headwater lake inlet	I6 headwater lake outlet	I6 Inlet West	I6 Outlet	I7 into I9	I7 Outlet	I8 Headwaters	I8 Inlet	I8 into I9	I8 Outlet	Island Lake	Milkyway upper	Milkyway Lower	This site is near the NE 14 Lake Slump Inlet and is different than the NE 14 site (which is out in the lake).	NE 14 lake slump inlet	Surveyed by Sarah Barbrow in 2009, Kling,Cory,Nannen, and Crump in 2010.	Toolik Inlet	TW Weir
Location Bounding Box
West Bounding Coordinate
East Bounding Coordinate
North Bounding Coordinate
South Bounding Coordinate
OR if single point location
Latitude	68.6256	68.6	68.53698	68.55634	68.55361	68.54593	68.61035	68.611683	68.574	68.572296	68.57478333	68.572546	68.57754	68.58423333	68.58143	68.59491667	68.589087	68.578643	68.58357	68.59688333	68.598684	68.61818333	68.60183	68.5736666666667	68.60853333	68.61838333	68.6114075339	68.52364	68.62016667	68.622	68.674932	68.677723	68.632586	68.6256	68.623488
Longitude	-149.59605	-149.183333333333	-149.2374	-149.56628	-149.53397	-149.54214	-149.599766	-149.599254	-149.583566	-149.581014	-149.58205	-149.570268	-149.582003	-149.5836	-149.5861	-149.586316	-149.589219	-149.621102	-149.62384	-149.60125	-149.599853	-149.596766	-149.596713	-149.537166666667	-149.587633	-149.5965	-149.590131872	-149.48141	-149.568166	-149.590666	-149.625439	-149.624028	-149.600895	-149.59605	-149.616559
Elevation	719	864	883	801	820	852	736	736	774	785	774	785	774	770	770	754	767	808	808	754	754	728	742	808	744	728	744	881	739	725	701	701	719	719	761
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TAXONOMIC COVERAGE:
Organisms studied
Methods:	In addition to the link above, the chlorophyll protocol is available in the Land-Water protocol accessible from the Land-Water main page on the Arctic LTER web page or at http://www-personal.umich.edu/~gwk and clicking on the protocol link at the bottom of the page. This method is adapted from EPA Method 445.0, Arar, J and G. B. Collins. In Vitro Determination of Chl a and Pheo a in Marine and Freshwater Algae by Fluorescence. Revision 1.9, 1997. In brief, sample water is filtered through Whatman GF/F 25 mm or 47 mm (0.7um pore) glass fiber filters. Filters are frozen for preservation at -80 C for a period of 24 hours to two weeks. Chl a is then extracted by submersing filters in 90% acetone buffered with MgCO3 for a 24 hour incubation period. Extracted chlorophyll samples are then read on a Turner Designs10-AU Fluorometer before and after acidification with 100uL 0.1N HCl. Note: Acidified and non-acidified sample readings are used to calculate phaeophytin concentrations and correct chlorophyll a values. This acidification technique was originally tested during part of the 2000 sample season and has been used consistently for chlorophyll a analysis by the Landwater Group from 2002 to present. Methodology was formally adopted by the LTER (including the Lakes Group) in 2004.

Data Table

Variable Name	Variable Description	Data Type	Units	DateTime Format	Code Information	Missing Value Code
SortChem	Yearly number assigned to samples collected at a specific site at a specific date and time. The year that the sample was collected is followed by a dash and then a sequential number. For example, if 600 samples were collected in 2002, then the first sample collected is 2002-0001 and the last sample is 2002-0600.	text
Site	Site Name. If a soil site, the site is described in the name by general location then specific feature (watertrack,etc) then specific point (1,2,3,...). If a lake or streams site, the lake or stream name (as it is commonly known) is given. Exceptions in naming procedure are provided in the Code Information.	text			TW=Tussock Watershed| WT=Watertrack| RE=Rain Event| I=I series lakes and inlets/outlets
Date	Sampling Date	datetime		dd-mmm-yy		.
Time_hr_dst	Sampling Time in Alaska Daylight Savings Time (1 hour ahead of Alaska Standard Time: if 13:00 DST than 12:00 AST)	datetime		hh:mm		.
Depth_m	Depth from surface of lake, stream, or soil	number	meter			.
Chla_Phaeophytin_Corrected_ugL	Fluorescence estimate of Chlorophyll a corrected for phaeopigment (phaeophytin). This acidification method was started in 1999, and applied to all samples in this spreadsheet (landwater) by 2000. Note that the lakes Arcitc LTER group started this method	number	microgramPerLiter			.
Phaeopigment_ugL	Fluorescence estimate of Phaeopigment estimated by acidification of a chlorophyll sample.	number	microgramPerLiter			.
Chla_Raw_ugL	Fluorescence estimate of Chlorophyll a without a phaeopigment correction (no acidification). This was this historical measure use by the Arctic LTER and previous studies of Toolik Lake. Chla_Raw = (((Sample Fluorescence - Blank) * Slope) - Intercept) * (Acetone_ml / Sample Volume Filtered)	number	microgramPerLiter			.
	Please contact arc_im@mbl.edu with questions, comments, or for technical assistance regarding this web site.