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Arctic LTER Database
Acceptance and utilization of LTER data requires that:
(1) The Principal Investigator be sent a notice stating reasons for acquiring any data and a description of the publication intentions.
(2) The Principal Investigator of the data set be sent a copy of the report or manuscript prior to submission and be adequately cited in any resultant publications.
(3) A copy of any resultant publications should be sent to:
Marine Biological Laboratory
7 MBL St.
Woods Hole, MA 02543
Older Metadata: Attribute table not in XML (EML) file
|Dataset URLs:||METADATA: HTML, Rich Text, XML(EML compliant)|
DATA: Comma Delimited, Excel file with Metadata and data
|Dataset Title:||Chlorophyll concentrations of Schistidium agassizii and Hygrohypnum spp, Arctic LTER 1996.|
|Investigator 1:|| |
|Address line 1:|
|Address line 2:|
|Address line 3:|
|Associate Investigators:||Dave Arscott, Jim Lee|
|Keywords:||chlorophyll, Kuparuk River, reference, recovery, fertilized, bryophytes, riffle, Hygrohypnum, Schistidium|
of Schistidium agassizii and Hygrohypnum spp. retrieved from the Kuparuk River. In
1996, weekly, from 22-Jun to 10-Aug, collections of bryophytes from 3 riffles each
treatment reach (i.e. reference, recovery, and fertilized).|
||Arctic LTER Information Manager
The Ecosystems Center
Marine Biological Lab
7 MBL St
Woods Hole, MA 02543
Phone (508) 289 7496
Online URL: http://ecosystems.mbl.edu/ARC/
|DATA FILE INFORMATION:
|Data File Name
|Number of Data Records
|Other Files to Reference
|Quality Control Information
|Log of Changes:
||Log of changes:
For Archival Use:
DATE RECEIVED: June 1997
DATA FILE ENTERED BY: Karie Slavik
DATA FILE VALIDATION:
Reference: riffles from 0.56 to 0.0 km
Recovery: riffles from 0.59 to 1.3 km
Fertilized: riffles from 0.6 to 7.5 km
|Location Bounding Box
|West Bounding Coordinate
|East Bounding Coordinate
|North Bounding Coordinate
|South Bounding Coordinate
|OR if single point location
In 1996, we moved the Kuparuk River's point of
phosphorus addition 0.75 km downstream to stream benchmark +1.4 km. This created 3
treatment reaches: a reference reach (upstream of +0.59 km), a P-recovery reach (+0.59 to
+1.35 km), and a long-term P-fertilized reach (1.35 to 10 km). Further downstream
(typically > 10 km) nutrient conditions return to background concentrations (Peterson et
al. 1993, Bowden et al. 1994). From each of these treatment reaches we have chosen 3
riffles from which to sample.
For 8 consecutive weeks during the 1996 field season, total chlorophyll content of
S. agassizii and Hygrohypnum spp. was determined. Each week collections of S.
agassizii were retrieved from 3 riffles in each treatment reach and collections of
Hygrohypnum spp. were retrieved from each of the designated 3 riffles in the recovery
and fertilized reaches. Hygrohypnum spp. is rarely found in the reference zone and was
therefore not included. Two, small, thumb-sized clumps of each species were randomly
collected from each of the study riffles, rinsed to remove loose epiphytes, detritus, and
invertebrates, and placed in a whirl-pak bag and transported to the laboratory. Because S.
agassizii can be found on rocks that are more frequently exposed to the atmosphere than
Hygrohypnum spp. an effort was made to collect clumps from permanently submerged
At the laboratory 1 frond from each of the collected clumps was randomly
selected for chlorophyll extraction. Each frond was patted dry and weighed on a Cahn C-
33 microbalance to record the fresh mass and then placed in 10 ml of 100 % DMSO for
40-48 hours for chlorophyll extraction (Alpert 1984, Hains 1985, Spencer and Ksander
1987). Chlorophyll was determined spectrophotometrically following methods and
equations of Golterman (1969). Absorbency was read at 665 nm and (to correct for
turbidity) at 750 nm. Samples were not acidified and thus, measurements represent total
Ten additional fronds from each species were selected to develop a wet weight to
dry weight relationship. The fronds ranged in size from small to large and each frond
was weighed separately. After weighing, all fronds were dried at 65.0 oC to a consistent
weight and re-weighed individually. Regression analysis was used to quantify the wet to
dry relationship for each species. This relationship was established weekly for both
Total chlorophyll concentrations were converted to dry mass specific estimates
using the established wet to dry weight relationship and the equation is described below
Calculations: Cd * Vd
::::- = CHLt Eq. 1
where: CHLt = moss total chlorophyll (mg CHLt / gdw)
Cd = chlorophyll concentration in DMSO (mg CHLt / L)
Vd = volume of DMSO (0.01 L)
m = dry mass from wet dry relationship (g)
Reference Citations: Arscott, D. B. 1997. Primary Production in an Arctic Stream Ecosystem. Thesis,
University of New Hampshire.
Manuscript submitted. 1997. Arscott, Dave B., W. B. Bowden, and J. C. Finlay.
Epilithic algal and bryophyte metabolism in an arctic tundra stream, Alaska.
Manuscript submitted. 1997. Arscott, Dave B., W. B. Bowden, J. C. Finlay, and J. Lee.
Ecophysiology of two arctic stream bryophytes.
Alpert, P. 1984. Analysis of chlorophyll content in mosses through extraction in DMSO.
The Bryologist 87(4):363-365.
Bowden, W. B., J. C. Finlay, and P. E. Maloney. 1994. Long-term effects of PO4
fertilization on the distribution of bryophytes in an arctic river. Freshwater Biology
Bowden, W.B., B.J. Peterson, J.C. Finlay, and J. Tucker. 1992. Epilithic chlorophyll a,
photosynthesis, and respiration in control and fertilized reaches of a tundra stream.
Hydrobiologia 240: 121-131.
Finlay, J. C. and W. B. Bowden. 1994. Controls on production of bryophytes in an arctic
tundra stream. Freshwater Biology 32:455-466.
Golterman, 1969. Studies on the cycle of elements in fresh water. Acta Botanica
Hains, J. J. 1985. Practical considerations for routine chlorophyll measurements:
Precautions and comparison of extraction methods. Journal of Freshwater Ecology
Spencer, D. F., G. G. Ksander. 1987. Comparison of three methods for extracting
chlorophyll from aquatic macrophytes. Journal of Freshwater Ecology 4(2):201-208.
(11) VARIABLE DESCRIPTIONS:
Variable Variable description Units
SITE site of measurement y
D/D distance from K or O dripper 0.05 km
DATE date ddmmyy n
LOCATION location (REF, RECO, FERT)
SUBSITE typically POOL or RIFFLE
TYPE community description
CHLT total chlorophyll content mgCHLt/gdw