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Conditions of Use

The re-use of scientific data has the potential to greatly increase communication, collaboration and synthesis within and among disciplines, and thus is fostered, supported and encouraged. Permission to use this dataset is granted to the Data User free of charge subject to the following terms:

1) Acceptable use. Use of the dataset will be restricted to academic, research, educational, government, recreational, or other not-for-profit professional purposes. The Data User is permitted to produce and distribute derived works from this dataset provided that they are released under the same license terms as those accompanying this Data Set. Any other uses for the Data Set or its derived products will require explicit permission from the dataset owner.
2 ) Redistribution. The data are provided for use by the Data User. The metadata and this license must accompany all copies made and be available to all users of this Data Set. The Data User will not redistribute the original Data Set beyond this collaboration sphere.
3 ) Citation. It is considered a matter of professional ethics to acknowledge the work of other scientists. Thus, the Data User will properly cite the Data Set in any publications or in the metadata of any derived data products that were produced using the Data Set. Citation should take the following general form: Creator, Year of Data Publication, Title of Dataset, Publisher, Dataset identifier. For example:

Shaver, G. 1989. Above ground biomass in acidic tussock tundra experimental site, 1989, Arctic LTER, Toolik, Alaska. Arctic LTER, Marine Biological Lab, Woods Hole, Ma 02543. 1989gsttbm http://ecosystems.mbl.edu/arc/terrest/biomass/index.shtml 

4 ) Acknowledgement. The Data User should acknowledge any institutional support or specific funding awards referenced in the metadata accompanying this dataset in any publications where the Data Set contributed significantly to its content. Acknowledgements should identify the supporting party, the party that received the support, and any identifying information such as grant numbers. For example:

Data sets were provided by the Arctic LTER. This material is based upon work supported by the National Science Foundation under Grants #DEB-981022, 9211775, 8702328; #OPP-9911278, 9911681, 9732281, 9615411, 9615563, 9615942, 9615949, 9400722, 9415411, 9318529; #BSR 9019055, 8806635, 8507493.

5 ) Notification. The Data User will notify the Data Set Contact when any derivative work or publication based on or derived from the Data Set is distributed. The Data User will provide the data contact with two reprints of any publications resulting from use of the Data Set and will provide copies, or on-line access to, any derived digital products. Notification will include an explanation of how the Data Set was used to produce the derived work.
6 ) Collaboration. The Data Set has been released in the spirit of open scientific collaboration. Data Users are thus strongly encouraged to consider consultation, collaboration and/or co-authorship with the Data Set Creator.

By accepting this Data Set, the Data User agrees to abide by the terms of this agreement. The Data Owner shall have the right to terminate this agreement immediately by written notice upon the Data User's breach of, or non-compliance with, any of its terms. The Data User may be held responsible for any misuse that is caused or encouraged by the Data User's failure to abide by the terms of this agreement.

Disclaimer

While substantial efforts are made to ensure the accuracy of data and documentation contained in this Data Set, complete accuracy of data and metadata cannot be guaranteed. All data and metadata are made available "as is". The Data User holds all parties involved in the production or distribution of the Data Set harmless for damages resulting from its use or interpretation.

Dataset URLs:METADATA: HTML, Rich Text, XML(EML compliant)
DATA: Comma Delimited, Excel file with Metadata and data, Dataset via LTER Data Poral
Dataset ID:20032004gsimresin.03
Dataset Title:NO3 and NH4 collected by resin bags in 15N addition plots during 2003-2004
Investigator 1: 
First Name:Gus
Last Name:Shaver
Organization:Ecosystems Center
Address line 2:Marine Biological Lab
Address line 3:
City:Woods Hole
State:MA
Zip Code:02543
Country:USA
Investigator 2: 
First Name:Yuriko
Last Name:Yano
Organization:Ecosystems Center
Address line 2:Marine Biological Lab
City:Woods Hole
State:MA
Zip Code:02543
Country:USA
Investigator 3: 
First Name:Anne
Last Name:Giblin
Organization:
Address line 2:
City:
State:
Zip Code:
Country:
Investigator 4: 
First Name:Ed
Last Name:Rastteter
Investigator 5: 
First Name:Jim
Last Name:Laundre
Associate Investigators:
Keywords:resin bags, nitrate, ammonium, inorganic nutrients
Abstract:Concentrations of NO3 and NH4 and d15N of NO3 and NH4 collected on resin bags from 15N addition plots along hillslope in Imnavait watershed.
For questions about the Metadata and data contact the Investigators.
For information about this web site contact:
Arctic LTER Information Manager
The Ecosystems Center
Marine Biological Lab
7 MBL St
Woods Hole, MA 02543
Phone (508) 289 7496
Email: arc_im@mbl.edu
Online URL: http://ecosystems.mbl.edu/ARC/
DATA FILE INFORMATION:
Data File URL http://metacat.lternet.edu/das/dataAccessServlet?docid=knb-lter-arc.10183&urlTail=terrest/bulk/data/20032004gsimresin.csv
Data File Name 20032004gsimresin.dat
Beginning Date 7/29/2003
End Date 8/2/2004
Number of Data Records 244
Other Files to Reference
Availability Status 1
Quality Control Information
Maintenance Description
Log of Changes: Updated Metadata sheet
Version 2: Updated units to current standards. Missing values changed to #N/A. Updated metadata form to newer version (without site sheet). Fixed discrepency with header variable names. CH 24 Jan 2013 Updated to newer metadata with site sheet. CH March 2013.
Version 3: Checked keywords against the LTER network preferred list and replaced non-preferred terms. Jim L 27Jan14
 
RESEARCH LOCATION:                                            
Location Name Imnaviat watershed- eastern slope Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One Select Site or enter New One  
Geographic Description Eastern slope of Imnavait watershed. ~1km south of main weir, ~4 km south of Dalton Hwy. Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description Enter Description  
Location Bounding Box                                            
West Bounding Coordinate                                            
East Bounding Coordinate                                            
North Bounding Coordinate                                            
South Bounding Coordinate                                            
OR if single point location                                            
Latitude 68.6081 In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees  
Longitude -149.3078 In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees In Decimal Degrees  
Elevation In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters In Meters  
Link to Google Map View on Google Map                                          
                                             
 
TAXONOMIC COVERAGE:
Organisms studied
 
Methods:Field:
Five resin bags (~8mL resin/bag), except when it is specified, were deployed at each location, and the bags were left in the field for ~3 weeks each time. Bags were deployed at ~10cm deep soil, except for snowmelt bags which were placed at the bottom of snow pack in early May then pushed into thawed moss layer as the soil melted. All bags were kept at ~5-degrees C until extraction.

Lab:
Five bags were pooled, then 2N KCl (500 mL for NO3 and 400mL for NH4) was used to completely replace NO3 or NH4 accumulated on the resins. NO3 and NH4 concentrations were determined (see below) then d15N values were determined using LINX2 diffusion method (see below).

NO3: Cd reduction followed by Griess-Ilosvay method for NO2
NH4: Berthelot method

d15N for NO3:
1. Measure out the sample volume to be boiled down and add to a clean, acid-washed beaker or flask.
2. Add about 5g of ashed NaCl (for ionic balance, want final concentration after boil down to be about 50 g/L) and about 3 g ashed MgO (to raise pH) to sample (add proportionately more NaCl if boiling down smaller volumes of sample to achieve a 50 g/L concentration after boil-down, add same amount of MgO regardless of sample size to boil down).
3. Add stir bar and place on hot plate with stirring capability. Heat until volume is reduced to roughly 100 ml (doesn’t have to be exact, 75-125 mLs should be okay). Aim for a simmering boil, but if just below this then that is fine as well. Stir during the boil. It takes about 3-4 hours of gentle boiling to reduce 1 L to about 100 mL.
4. After cooling somewhat, pour the boiled down sample into an acid-washed 250 mL HDPE bottle (suggest rigid rectangular bottles). These can be stored in refrigerator until you have an entire series (stream) ready for next step (reduction of nitrate to NH3 and diffusion of NH3 into headspace).
5. Add about 0.5 g of ashed MgO and then 0.5 g of Devardas alloy to boiled-down sample in 250 mL bottle.
6. Immediately after adding Devardas alloy, place filter pack (see subsection on steps for constructing filter packs below) carefully on surface of water, and cap very tightly.
7. Place bottles in oven at 60oC for 48 hours (be careful not to pack too closely in a tray since the bottles will swell slightly).
8. Remove from oven and place bottles on shaker and shake gently for 7 days at room temperature.
9. Open bottles and remove filter pack. Blot water droplets from filter pack and place in labeled scintillation vial and into dessicator. Also place an open vial of 2.5 M KHSO4 (to absorb any ammonium in air) in dessicator.
10. Let filters dry in dessicator for 4 days or so. Remove and cap the scintilation vials containing filter packs very tightly (use parafilm if necessary) and store until ready to encapsulate in tins.
11. Encapsulating filters: Remove filter pack from scintillation vial on clean surface (use alcohol to clean). Using cleaned forceps, open filter pack and remove small glass fiber filter. Place filter in tin capsule and fold opening of tin down once and compress. Crimp sides of tin to form small packet (all dimensions < 2 mm). Place tin packet into a well in the well tray recording the well location and sample ID (station, time). Place well cap strip over wells as soon as possible to minimize any further exposure of encapsulated filter to air. It is recommended that you use a separate well plate for the samples that will be only minimally enriched if at all (PRE, POST, and deionized water samples) and the enriched samples (collected during the 15N addition).

Reference: Sigman, D. M., M. A. Altabet, R. Michener, D. C. McCorkle, B. Fry, and R. M. Holmes. 1997. Natural abundance-level measurement of nitrogen isotopic composition of oceanic nitrate: an adaptation of the ammonia diffusion method. Marine Chemistry 57:227-242. (Note that the methods outlined here is developed for LINX II project and based on the Sigman et al. paper, but are not exactly the same. In particular, the LINX II protocol does not go through extra incubation steps to reduce blank effects caused by breakdown of DON. Instead, DON blank effects are minimized by running diffusions at a lower incubation temperature than is used in the Sigman et al. paper.)



d15N for NH4:
1. Measure 100mL of sample to an acid-washed 250 mL HDPE bottle.
2. Add about 0.5 g of ashed MgO and filter packet, cap tightly, then incubate at 40oC for 7 days. Follow NO3 method (#9).

Data Table

Variable Name Variable Description Data Type Units DateTime Format Code Information Missing Value Code
Sample No. serial numbers for the extracts number number      
Date Date of resin bag retrieval datetime   DD-MMM-YYYY    
Plot resin bag deployment sites along the hillslope text        
Location CR=crest; LS NWT= lower slope non-watertrack; LS WT=lower slope water; FS WT=foot slope watertrack; FS NWT=foot slope non-watertrack; R=riparian wet sedge text        
Distance distance below (shonw as positive values) and above (shown as negative values) the plots text        
Season season when resin bags were deployed text        
Chemform chemical forms of N (either NO3 or NH4) text        
Conc N concentration of resin extracts in uM (either NO3 or NH4). DON contamination is not an issue for these values. number micromole      
Bag No. number of bags deployed. 5 bags were deployed if not mentioned. number number     #N/A=Missing or Not Measured
d15N del-15N values (o/oo) number partPerThousand     #N/A=Missing or Not Measured
Note DON contamination occurred. The contamination accounted for as much as 900% (for NH4) and 4000% (for NO3) of targeted DIN. Only values without "DON contamination" should be used as d15N for DIN. All DIN concentrations are valid. text        

Please contact arc_im@mbl.edu with questions, comments, or for technical assistance regarding this web site.