MBL Stable Isotope Laboratory

The Ecosystems Center, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543 U.S.A.
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Lab Manager:
Marshall Otter, Ph.D.
Office: 508-289-7462
Lab: 508-289-7762
Fax: 508-457-1548
E-mail: motter@mbl.edu

About the Lab

Dr. Brian Fry established the MBL Stable Isotope Laboratory at The Ecosystems Center in 1985.   The first Isotope Ratio Mass Spectrometer (IRMS), a dual-inlet Finnigan MAT 251, was purchased with a NSF grant and matching funds from the A. W. Mellon Foundation.  With the help of Bob Michener, the lab quickly started producing high quality δ15N, δ13C and δ34S data on ecological sample materials.


Fry and Michener in 1985 (before color photography) with new Finnigan MAT 251

In 1989, Hap Garritt and Kris Tholke assisted Brian in setting up one of the first automated IRMS systems with the newly acquired Finnigan Mat Delta S dual-inlet mass spectrometer and its innovative "trapping box" (a combustion & cryogenic gas purification unit) purchased with a grant from NSF.  This system, for the first time, allowed the simultaneous analysis of δ15N and δ13C on individual sample aliquots (Fry et al., Analytical Chemistry 64, 1992.)

Tholke assumed management of the lab in 1994 and, in 1997, she set up the Europa 20-20 continuous flow-IRMS system that was purchased with a NSF grant studying nitrate uptake and retention in streams.  This continuous-flow system, which greatly increased sample throughput, was initially dedicated to δ15N tracer study samples.

In 2000, Marshall Otter was hired to manage the laboratory.  With the trapping box on its last legs, the Europa 20-20 became the workhorse system for doing δ15N and δ13C analyses.  The occasional gas samples submitted to the lab were processed on the Delta S. In 2001, the stable isotope lab was moved to the new C.V. Starr building on the MBL campus which also houses the offices and general use labs of The Ecosystems Center.

In 2005, the Delta S was sold to make room for two new GV Instruments Isoprime continuous flow-IRMS systems and five automated sample preparation units that were purchased with funds from a NSF grant awarded to Paul Steudler.  The new systems have greatly expanded the lab's capabilities, allowing the analysis of δ15N, δ13C, δ18O and δ2H on a larger variety of ecological samples, including water, DIC/DOC, trace gases, and specific compounds in organic materials.