TIDE Project Database

http://ecosystems.mbl.edu/tide

 

 

Acceptance and utilization of TIDE project data requires that:

·        The Project Principal Investigator, Linda Deegan, be sent a notice stating reasons for acquiring any data and a description of the publication intentions.

·        The Project PI and the Principal Investigator of the data set be sent a copy of the report or manuscript prior to submission and be adequately cited in any resultant publications.

·        A copy of any resultant publications should be sent to:

Linda Deegan
Ecosystems Center
Marine Biological Laboratory
Woods Hole, MA 02543

 

 

TIDE PROJECT DATABASE

DATA FILE:                                        LD-All-Benthic chl a-2003.xls

                                                            LD-All-Benthic chl a-2003.txt

DOCUMENTATION FILE:                LD-All-Benthic chl a-2003.doc

YEAR: 2003

PI: Linda Deegan

OTHERS: Christian Picard, Mike Johnson

BRIEF DESCRIPTION OF DATA FILE: Benthic chlorophyll samples collected from TIDE experimental and reference creeks.

KEYWORDS: sweeney, clubhead, west, nelson, benthic, chlorophyll a, TIDE

RESEARCH LOCATION:

Creek

Branch

Transect

Habitat

SW, CL, NE, WE

Right  Left, Confluence

1, 2, 3

MF, FA, TSA, SP, SSA

 

EXPERIMENTAL DESIGN AND METHODS: Benthic chlorophyll samples colleted using a core taken to 1 cm in depth.  Two replicate samples taken for each creek/branch/transect/habitat.  Samples frozen and analyzed later in laboratory.

NOTES AND COMMENTS:

VARIABLE DESCRIPTION:SW and WE creek were the nutrient addition manipulation, right branches of all creeks were fish removal manipulation

Name

Description

Unit

Precision

Year

Year sites were sampled

yyyy

 

Month

Month sites were sampled

 

 

Creek

Creek samples were collected from

 

 

Branch

Branch samples were collected from

 

 

Transect

Transect samples were collected from

 

 

Habitat

Habitat samples were collected from

 

 

Chl a

Amount of chl a present

mg/m2

 

Phaeophytin

Amount of phaeophytin present

mg/m2

 

 

 

 

 

 

 

 

 

Comments

 

 

 

 

CALCULATIONS: Chlorophyll and phaeophytins measured using a spectrophotometer.  Extractions from sediment made with 100% acetone, 12 hour soak time.

                                Chl A (ug/cc) =( (26.7 (665o – 665a) (v)) / (V)(L)

                                Phaeophytins (ug/cc) = (26.7((1.7 (665a)) – (665o)(v)) / (V)(L)

                                665o = turbidity corrected extinction at 665 nm before acidification

                                665a = turbidity corrected extinction at 665 nm after acidification

                                v = volume in ml of acetone during extraction, incl. mgCO3

                                V = volume inn cc of sediment extracted

                                26.7 = constant related to the specific absorbance of chl a

                                1.7 = ratio of the absorbance of pure chl a to the absorbance of an equivalent amount of

                 phaeophytin ( here chlorophyll is degraded by HCl) 

FOR MORE INFORMATION, CONTACT: Christian Picard

OTHER DATA FILES TO REFERENCE:

REFERENCE CITATIONS:

ARCHIVE INFORMATION:

Date of entry: 2/8/06                

Data entered by: Mike Johnson

Comments: