DNA
extraction from sediments Equipment: Solutions: Procedure: From
Lin, C. and D.A. Stahl. 1995. Taxon specific
probes for the cellulytic genus Fibrobacter
reveal abundant and novel equine-associated
populations. Appl. Environ. Microbiol.
61:1348-1351
Michele Bahr, April 2000
10 x Extraction buffer pH 8.0 (500 mM NaAcetate,
10 mM EDTA)
1 x buffer pH 8.0 (50 uM NaAcetate, 1
mM EDTA)
20% SDS (N-Lauroylsarcosine, e.g., Sigma #L9150
or L-7414)
Phenol, equilibrated, pH 8.0 (Sigma
#P4557)
Phernol: chloroform:isoamyl alcohol mixture at
25:24:1, equilibrated, pH 8.0 (Sigma #P2069)
Chloroform:isoamyl alcohol mixture at 25:24:1,
pH 8.0 (e.g., Sigma # CO549)
7.5 M NH4Acetate
100% isopropanol at room temperature
80% ethanol
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